Rapid detection of a chromosomally mediated penicillin resistance- associated ponA mutation in Neisseria gonorrhoeae using a real-time PCR assay

FEMS Microbiology Letters

Volumn 255, Issue 1, 2006, Pages 66-74

  Vernel Pauillac, Frédérique ^a   Falcot, Virginie ^a   Whiley, David ^b   Merien, Fabrice ^a  

^a Institut Pasteur de Nouvelle Caledonie Noumea   (New Caledonia)

^b Royal Children's Hospital and Health Service District   (Australia)

Author keywords

Mutation; Neisseria; ponA; Real time assay

Indexed keywords

PENICILLIN G;

ACCURACY; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CONTROLLED STUDY; CORRELATION ANALYSIS; FEMALE; FLUOROMETRY; GENE MUTATION; HUMAN; MAJOR CLINICAL STUDY; MALE; MUTATIONAL ANALYSIS; NEISSERIA GONORRHOEAE; NONHUMAN; NUCLEOTIDE SEQUENCE; PONA GENE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SEQUENCE ANALYSIS;

BACTERIA (MICROORGANISMS); NEISSERIA; NEISSERIA GONORRHOEAE;

EID: 33645078631     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2005.00053.x     Document Type: Article

References (33)

1. Ameyama S Onodera S Takahata M Minami S Maki N Endo K Goto H Suzuki H Oishi Y 2002 Mosaic-like structure of penicillin-binding protein 2 gene (penA) in clinical isolates of Neisseria gonorrhoeae with reduced susceptibility to Cefixime Antimicrob Agents Chemother 46 3744 3749
2. Bala M Ray K Gupta SM 2005 Comparison of disc diffusion results with minimum inhibitory concentration (MIC) values for antimicrobial susceptibility testing of Neisseria gonorrhoeae Indian J Med Res 122 48 51
3. Barratt K Mackay JF 2002 Improving real-time PCR genotyping assays by asymmetric amplification J Clin Microbiol 10 1571 1572
4. Bowler LD Zhang QY Riou JY Spratt BG 1994 Inter-species recombination between the penA genes of Neisseria meningitidis and commensal Neisseria species during the emergence of penicillin resistance in Neisseriameningitidis natural events and laboratory simulation J Bacteriol 176 333 337
5. Brannigan JA Tirodimos IA Zhang QY Dowson CG Spratt BG 1990 Insertion of an extra amino acid is the main cause of the low affinity of penicillin-binding protein 2 in penicillin-resistant strains of Neisseria gonorrhoeae Mol Microbiol 4 913 919
6. Centers for Disease Control and Prevention 1997 Gonorrhea among men who have sex with men-selected sexually transmitted diseases clinics, 1993-1996 MMWR 46 889 892
7. Comité de l'Antibiogramme de la Société Française de Microbiologie 1996 Technical recommendations for in vitro susceptibility testing Clin Microbiol Infect 2 Suppl S11 S25 Updates on
8. Dougherty TJ 1983 Peptidoglycan biosynthesis in Neisseria gonorrhoeae strains sensitive and intrinsically resistant to β-lactam antibiotics J Bacteriol 153 429 435
9. Dougherty TJ 1986 Genetic analysis and penicillin-binding protein alterations in Neisseria gonorrhoeae with chromosomally mediated resistance Antimicrob Agents Chemother 30 649 652
10. Van Dyck E Smet H Piot P 1994 Comparison of E test with agar for antimicrobial susceptibility testing of Neisseria gonorrhoeae J Clin Microbiol 32 1586 1588
11. Easmon CSF 1990 The changing pattern of antibiotic resistance of Neisseria gonorrhoeae Genitourin Med 66 55 56
12. Fredlund H Falk L Jurstrand M Unemo M 2004 Molecular genetic methods for diagnosis and characterisation of Chlamydia trachomatis and Neisseria gonorrhoeae impact on epidemiological surveillance and interventions APMIS 112 771 784
13. Galvin SR Cohen MS 2004 The role of sexually transmitted diseases in HIV transmission Nat Rev Microbiol 2 33 42
14. Hagman KE Pan W Spratt BG Balthazar JT Judd RC Shafer WM 1995 Resistance of Neisseria gonorrhoeae to antimicrobial hydrophobic agents is modulated by the mtrRCDE efflux system Microbiology 141 611 622
15. Herida M Sednaoui P Goulet V 2004 Gonorrhoea surveillance system in France 1986-2000 Sex Transm Dis 4 209 214
16. Ison CA Pepin J Roope NS Secka O Easmon CSF 1992 The dominance of a multiresistant strain of Neisseria gonorrhoeae among prostitutes and STD patients in the Gambia Genitourin Med 68 356 360
17. Ito M Yasuda M Yokoi S Ito S Takahashi Y Ishihara S Maeda S Deguchi T 2004 Remarkable increase in Central Japan in 2001-2002 of Neisseria gonorrhoeae isolates with decreased susceptibility to penicillin, tetracycline, oral cephalosporins, and fluoroquinolones Antimicrob Agents Chemother 48 3185 3187
18. Kahlmeter G Brown DFJ Goldstein FW MacGowan AP Mouton JW Osterlund A Rodloff A Steinbakk M Urbaskova P Vatopoulos A 2003 European harmonization of MIC breakpoints for antimicrobial susceptibility testing of bacteria J Antimicrob Chemother 52 145 148
19. Li Z Yokoi S Kawamura Y Maeda SI Ezaki T Deguchi T 2002 Rapid detection of quinolone resistance-associated gyrA mutations in Neisseria gonorrhoeae with a LightCycler J Infect Chemother 8 145 150
20. Lujan R Zhang QY Sáez Nieto JA Jones DM Spratt BG 1991 Penicillin-resistant isolates of Neisseria lactamica produce altered forms of penicillin-binding protein 2 that arose by interspecies horizontal gene transfer Antimicrob Agents Chemother 35 300 3004
21. Lyon E 2001 Mutation detection using fluorescent hybridization probes and melting curve analysis Expert Rev Mol Diagn 1 92 101
22. Mavroidi A Tzouvelekis LS Kyriakis KP Avgerinou H Daniilidou M Tzelepi E 2001 Multidrug-resistant strains of Neisseria gonorrhoeae in Greece Antimicrob Agents Chemother 45 2651 2654
23. Olesky M Hobbs M Nicholas RA 2002 Identification and analysis of amino acid mutations in porin IB that mediate intermediate-level resistance to penicillin and tetracycline in Neisseria gonorrhoeae Antimicrob Agents Chemother 46 2811 2820
24. Overbergh L Giuletti A Valckx B Decallonne B Bouillon R Mathieu C 2003 The use of real-time reverse transcriptase PCR for the quantification of cytokine gene expression J Biomol Tech 14 33 43
25. Ropp PA Nicholas RA 1997 Cloning and characterization of the ponA gene encoding penicillin-binding protein 1 from Neisseria gonorrhoeae and Neisseria meningitidis J Bacteriol 179 2783 2787
26. Ropp PA Hu M Olesky M Nicholas RA 2002 Mutations in ponA, the gene encoding penicillin-binding protein 1, and a novel locus, penC, are required for high-level chromosomally mediated penicillin resistance in Neisseria gonorrhoeae Antimicrob Agents Chemother 46 769 777
27. Saribas Z Yurdakul P Alp A Gunalp A 2005 Use of fluorescence resonance energy transfer for rapid detection of isoniazid resistance in Mycobacterium tuberculosis clinical isolates Int J Tuberc Lung Dis 2 181 187
28. Spratt BG Zhang QY Jones DM Hutchison A Brannigan JA Dowson CG 1989 Recruitment of a penicillin-binding protein gene from Neisseria flavescens during the emergence of penicillin resistance in Neisseria meningitidis Proc Natl Acad Sci USA 86 8988 8992
29. Stefanelli P Caratolli A Neri A Fazio C Mastrantonio P 2003 Prediction of decreased susceptibility to penicillin of Neisseria meningitidis strains by real-time PCR J Clin Microbiol 41 4666 4670
30. Tipper DJ Strominger JL 1965 Mechanism of action of penicillins a proposal based on their structural similarity to acyl- d -alanyl- d -alanine Proc Natl Acad Sci USA 54 1133 1141
31. Veal WL Nicholas RA Shafer WM 2002 Overexpression of the MtrC- MtrD- MtrE efflux pump due to an mtrR mutation is required for chromosomally mediated penicillin resistance in Neisseria gonorrhoeae J Bacteriol 184 5619 5624
32. Wang SA Lee MVC O'Connor N et al 2003 Multidrug-resistant Neisseria gonorrhoeae with decreased susceptibility to Cefixime-Hawaii, 2001 Clin Infect Dis 37 849 852
33. Whiley DM Buda PJ Bayliss J Cover L Bates J Sloots TP 2004 A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene Eur J Clin Microbiol Infect Dis 23 705 710