Evaluation of acid phosphatase as a confirmation test for Clostridium perfringens isolated from water

Letters in Applied Microbiology

Volumn 42, Issue 4, 2006, Pages 418-424

  Sartory, D P ^a,b   Waldock, R ^a   Davies, C E ^a   Field, A M ^a  

^a SEVERN TRENT WATER LTD   (United Kingdom)

^b SWM Consulting   (United Kingdom)

Author keywords

Acid phosphatase; Clostridium perfringens; Confirmation tests; Tryptose sulfite cycloserine agar

Indexed keywords

ALGAE; FLUORESCENCE; PHOSPHATASES; POLYSACCHARIDES; STRAIN;

% REDUCTIONS; ACID PHOSPHATASE; CLOSTRIDIUM PERFRINGENS; CONFIRMATION TEST; CYCLOSERINE; NITRATE REDUCTION; PHOSPHATASE PRODUCTION; REFERENCE STRAINS; SULPHITES; TRYPTOSE SULPHITE CYCLOSERINE AGAR;

CLOSTRIDIUM;

4 METHYLUMBELLIFERYLPHOSPHATE; ACID PHOSPHATASE; AGAR; COUMARIN DERIVATIVE; GELATIN; LACTOSE; NITRATE; SULFITE; TRYPTOSE SULFITE CYCLOSERINE AGAR; UNCLASSIFIED DRUG;

BACTERIA; BACTERIOLOGY; CHEMICAL ANALYSIS; ENZYMES; FERMENTATION; NITRATES; REDUCTION;

BACTERIUM; DETECTION METHOD; PHOSPHATASE;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BACTERIUM ISOLATION; BIOMASS FERMENTATION; CELL MOTILITY; CLOSTRIDIUM; CLOSTRIDIUM BARATII; CLOSTRIDIUM PERFRINGENS; CLOSTRIDIUM RECTUM; CULTURE MEDIUM; ENZYME SYNTHESIS; FERMENTATION; FLUORESCENCE; INCUBATION TEMPERATURE; INTERMETHOD COMPARISON; LIQUEFACTION; NITRATE METABOLISM; NONHUMAN; NUCLEOTIDE SEQUENCE; RELIABILITY; WATER ANALYSIS; WATER SAMPLING;

BACTERIA (MICROORGANISMS); CLOSTRIDIA; CLOSTRIDIUM; CLOSTRIDIUM BARATII; CLOSTRIDIUM PERFRINGENS; CLOSTRIDIUM RECTUM;

EID: 33645073037     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2006.01867.x     Document Type: Article

References (21)

1. Adcock P.W. Saint C.P. 2001 Rapid confirmation of Clostridium perfringens by using chromogenic and fluorogenic substrates Appl Environ Microbiol 67 4382 4384
2. Anon. 2004 The Microbiology of Drinking Water 2004 - Part 6 - Methods for the Isolation and Enumeration of Sulphite-Reducing Clostridia and Clostridium perfringens by membrane filtration. Methods for the Examination of Water and Associated Materials Nottingham Standing Committee of Analysts, The Environment Agency Available at: (accessed 9 February 2006)
3. Araujo M. Sueiro R.A. Gómez M.J. Garrido M.J. 2001 Evaluation of fluorogenic TSC agar for recovering Clostridium perfringens in groundwater samples Water Sci Technol 43 201 204
4. Araujo M. Sueiro R.A. Gómez M.J. Garrido M.J. 2004 Enumeration of Clostridium perfringens spores in groundwater samples: comparison of six culture media J Microbiol Methods 57 175 180
5. Barrow G.I. Feltham R.K.A. ed. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria 3rd edn Cambridge Cambridge University Press
6. Baumgart J. Baum O. Lippert S. 1990 Schneller und direkter nachweis von Clostridium perfringens Fleischwirtsch 70 1010 1014
7. Bisson J.W. Cabelli V.J. 1979 Membrane filter enumeration method for Clostridium perfringens Appl Environ Microbiol 37 55 66
8. Burger J.S. Nupen E.M. Grabow W.O.K. 1984 Evaluation of four growth media for the membrane filtration counting of Clostridium perfringens in water Water SA 10 185 188
9. Cato E.P. George W.L. Finegold S.M. 1986 Genus Clostridium Prazmowski 1880 In Bergey's Manual of Systematic Bacteriology Vol. 2 eds Sneath P.H.A. Mair N.S. Sharpe M.E. Holt J.G. pp 1141 1200 Baltimore, MD Williams and Wilkins
10. Eisgruber H. Reuter G. 1991 SCA - ein selektivnährmedium zum nachweis mesophiler sulfitreduzierender clostridien in lebensmitteln, speziell für fleisch und fleischerzeugnisse Arch Lebensmittelhyg 42 101 132
11. Eisgruber H. Reuter G. 1995 A selective medium for the detection and enumeration of mesophilic sulphite-reducing clostridia in food monitoring programs Food Res Intern 28 219 226
12. Eisgruber H. Schalch B. Sperner B. Stolle A. 2000 Comparison of four routine methods for the confirmation of Clostridium perfringens in food Int J Food Microbiol 57 135 140
13. Eisgruber H. Geppert P. Sperner B. Stolle A. 2003 Evaluation of different methods for the detection of Clostridium perfringens phosphatases Int J Food Microbiol 82 81 86
14. ISO 2002 Water Quality - Detection and Enumeration of Clostridium perfringens- Part 2: Method by Membrane Filtration (Revision of ISO 6461-2:1986). Committee Draft ISO/CD 6461-2
15. Mead G.C. De Leon L.P. Adams B.W. 1981 A study of rapid and simplified confirmatory tests for Clostridium perfringens J Appl Bacteriol 51 355 361
16. Sartory D.P. 1986 Membrane filtration enumeration of faecal clostridia and Clostridium perfringens in water Water Res 10 1255 1260
17. Sartory D.P. Watkins J. 1999 Conventional culture for water quality assessment: is there a future J Appl Microbiol Symp Suppl 85 225S 233S
18. Sartory D.P. Pritchard A.M. Holmes P. 1993 Enumeration of sulphite-reducing clostridia from potable water supplies Water Sci Technol 27 279 282
19. Sartory D.P. Field M. Curbishley S.M. Pritchard A.M. 1998 Evaluation of two media for the membrane filtration enumeration of Clostridium perfringens from water Lett Appl Microbiol 27 323 327
20. Schallehn G. Brandis H. 1973 Phosphatase-reagent for quick identification of Clostridium perfringens Zentralbl Bakteriol [Orig A] 225 343 345
21. Ueno K. Fujii H. Marui T. Takahashi J. Sugitani T. Ushijima T. Suzuki S. 1970 Acid phosphatase in Clostridium perfringens, a new rapid and simple identification method Jpn J Microbiol 14 171 173