Preparation and application of novel EOPO-IDA-metal polymer as recyclable metal affinity ligand in aqueous two-phase systems

Industrial and Engineering Chemistry Research

Volumn 45, Issue 5, 2006, Pages 1774-1779

  Lu, Jin ^a   Lin, Dong Qiang ^a   Yao, Shan Jing ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CHELATION; COPOLYMERS; PH EFFECTS; POLYMERS; PROTEINS; SYNTHESIS (CHEMICAL);

ETHYLENE OXIDE; METAL AFFINITY LIGANDS; METAL POLYMERS; TWO-PHASE SYSTEMS;

PHASE SEPARATION;

CHELATION; COPOLYMERS; PH EFFECTS; PHASE SEPARATION; POLYMERS; PROTEINS; SYNTHESIS (CHEMICAL);

EID: 33645015007     PISSN: 08885885     EISSN: None     Source Type: Journal    
DOI: 10.1021/ie051044p     Document Type: Article

References (19)

1. Birkenmeier, G.; Vijayalakshmi, M. A.; Stigbrand T.; Kopperschlager, G. Immobilized metal ion affinity partition-a method combing metal-protein interaction and partitioning of protein in aqueous in aqueous two-phase systems. J. Chromatogr. 1991, 539, 267.
2. Sivars, U.; Abramson, J.; Iwata, S.; Tjerneld, F. Affinity partitioning of a poly(histidine)-tagged integral membrane protein, cytochrome bo3 ubiquinol oxidase, in a detergent-polymer aqueous two-phase system containing metal-chelating polymer. J. Chromatogr. B 2000, 743, 307.
3. Estela da Silva, M.; Teixeira Franco, T. Purification of soybean jeroxidase (Glycine max) by metal affinity partitioning in aqueous two-phase systems. J. Chromatogr. B 2000, 743, 287.
4. Sharma, S.; Agarwal, G. P. Interactions of Proteins with Immobilized Metal Ions: A Comparative Analysis Using Various Isotherm Models. Anal. Biochem. 2001, 288, 126.
5. Persson, J.; Kaul, A.; Tjerneld, F. Polymer recycling in aqueous two-phase extractions using thermoseparating ethylene oxide-propylene oxide copolymers. J. Chromatogr. B 2000, 743, 115.
6. Johansson, H.-O.; Karlstrom, G.; Tjerneld, F. Temperature-induced phase partitioning of peptides in water solutions of ethylene oxide and propylene oxide random copolymers. Biochim. Biophys. Acta 1997, 1335, 315.
7. Persson, J.; Nystrom, L.; Ageland, H.; Tjerneld, F. Purification of recombinant apolipoprotein A-1Milano expressed in Escherichia coli using aqueous two-phase extraction followed by temperature-induced phase separation. J. Chromatogr. B 1998, 711, 97.
8. Persson, J.; Nystrom, L.; Ageland H.; Tjerneld, F. Purification of recombinant proteins using themoseparating aqueous two-phase system and polymer recycling. J. Chem. Technol. Biotechnol. 1999, 74, 238.
9. Li, M.; Zhu, Z.-Q.; Rodrigues, A. E. Process integration of separation of amino acids by a temperature-induced aqueous two-phase system. Ind. Eng. Eng. Res. 2002, 41, 251.
10. Kepka, C.; Collet, E.; Persson, J.; Stahl, A.; Lagerstedt, T.; Tjerneld, F.; Veide, A. Pilot-scale extraction of an intracellular recombinant cutinase from E. coli cell homogenate using a thermoseparating aqueous two-phase system. J. Biotechnol. 2003, 103, 165.
11. Lin, D.-Q.; Yao, S.-J.; Mei, L.-H.; Zhu, Z.-Q. Preparation of iminodiacetic acid-polyethylene glycol for immobilized metal ion affinity partitioning. Chin. J. Chem. Eng. 2000, 8, 310.
12. Zhang, Z.-X.;.Zhang, R.-H. Quantitative Analysis of Organic Group; Chemical Industry Press: Beijing, 1990.
13. Asturp, T.; Müllertz, S. The fibrin plate method Cor the estimating fibrinolytic activity. Arch. Biochem. Biophys. 1952, 40, 346.
14. Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248.
15. Gaberc-Porekar, V.; Menart, V. Perspectives of immobilized-metal affinity chromatography. J. Biochem. Biophys. Methods 2001, 49, 335.
16. Wen, Y.-X.; Lin, D.-Q.; Lu, J.; Yao, S.-J. Synthesis Process of an Affinity Ligand for Aqueous Two-phase Partitioning-lminodiacetic Acid-Polyethylene Glycol. Chem. React. Eng. Technol. 2003, 19(2), 129-134.
17. Sumi, H.; Hamada, H.; Tsushima, H.; Mihara, H.; Muraki H. A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto: atypical and popular soybean food in the Japanese diet. Experientia 1987, 43, 1110.
18. Hu, S.; Yao, S.-J.; Mei, L.-H. Optimization of Submerged Fermentation of Nattokinase reduction by Bacillus subtilis with Response Surface Methodology. Food Ferment. Ind. 2003, 29, 13.
19. Lu, J.; Zhao, J.; Lin, D.-Q.; Yao, S.-J. Separation of nattokinase by immobilized metal ion affinity partitioning in aqueous two-phase systems. J. Chem. Eng. Chin. Univ. 2004, 18, 465.