Caged gene-inducer spatially and temporally controls gene expression and plant development in transgenic Arabidopsis plant

Bioorganic and Medicinal Chemistry Letters

Volumn 16, Issue 9, 2006, Pages 2470-2474

  Hayashi, Ken Ichiro ^a   Hashimoto, Kazuya ^a   Kusaka, Naoyuki ^a   Yamazoe, Atsushi ^a   Fukaki, Hidehiro ^b   Tasaka, Masao ^b   Nozaki, Hiroshi ^a  

^a OKAYAMA UNIVERSITY OF SCIENCE   (Japan)

^b NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Caged hormone; Gene expression; Light

Indexed keywords

DEXAMETHASONE; ESTRADIOL; STEROID HORMONE;

ARABIDOPSIS; ARTICLE; CONTROLLED STUDY; DRUG DESIGN; GENE EXPRESSION; GENE INDUCTION; NONHUMAN; PHOTOACTIVATION; PLANT DEVELOPMENT; ROOT DEVELOPMENT; TRANSACTIVATION; TRANSGENIC PLANT; ULTRAVIOLET IRRADIATION;

ARABIDOPSIS; DEXAMETHASONE; ESTRADIOL; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE EXPRESSION REGULATION, PLANT; IMIDAZOLES; MOLECULAR STRUCTURE; NITRO COMPOUNDS; PLANTS, GENETICALLY MODIFIED; STRUCTURE-ACTIVITY RELATIONSHIP; ULTRAVIOLET RAYS;

ARABIDOPSIS;

EID: 33644983980     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2006.01.103     Document Type: Article

References (24)

1. J. Zuo, Q.W. Niu, and N.H. Chua Plant J. 24 2000 265
2. A.A. Mills Genes Dev. 15 2001 1461
3. A.H. Brand, and N. Perrimon Development 118 1993 401
4. J. Haseloff Methods Cell Biol. 58 1999 139
5. R. Tsugeki, and N.V. Fedoroff Proc. Natl. Acad. Sci. U.S.A. 96 1999 12941
6. W.T. Monroe, M.M. McQuain, M.S. Chang, J.S. Alexander, and F.R. Haselton J. Biol. Chem. 274 1999 20895
7. H. Ando, T. Furuta, R.Y. Tsien, and H. Okamoto Nat. Genet. 28 2001 317
8. S.B. Cambridge, R.L. Davis, and J.S. Minden Science 277 1997 825
9. F.G. Cruz, J.T. Koh, and K.H. Link J. Am. Chem. Soc. 112 2000 8777
10. Y. Shi, and J.T. Koh ChemBioChem 5 2004 788
11. W. Lin, C. Albanese, R.G. Pestell, and D.S. Lawrence Chem. Biol. 9 2002 1347
12. K.H. Link, F.G. Cruz, H.F. Ye, K.E. O'reilly, S. Dowdell, and J.T. Koh Bioorg. Med. Chem. 12 2004 5949
13. A. Stutz, C. Hobartner, and S. Pitsch Helv. Chim. Acta 83 2000 2477
14. 9Na, 608.2272).
15. -1), respectively.
16. Light-regulation of gene expression and development in transgenic plants. Arabidopsis transgenic seedlings with pER8::GFP or pIAA14::GFP-mIAA14-GR were grown for 5 days on vertically oriented germination medium agar plates (GM, 0.5× Murashige and Skoog salts [Gibco-BRL, Gaithersburg, MD], 1% sucrose, 1× B5 vitamins, and 0.2 g/L 2-(4-morpholino)-ethane sulfonic acid (Mes), pH 5.8). The seedlings were immersed in a liquid GM containing 20 μM caged ERs (1 or 2) or 50 μM of caged DEX (3) for 30 min. After washing with a fresh GM, the seedlings were placed on GM agar solidified over slide glass. The UV light was irradiated to root on a spot for 3 s by a fluorescent microscope (Olympus BX50) with a UV-band pass filter (360-370 nm). The irradiated seedlings were transferred to a GM agar plates and then incubated vertically for an additional 12 h in darkness. For control treatment, 5-day-old transgenic or wild-type seedlings were transferred to GM agar plate containing 0, 1 μM ER or 10 μM DEX and grown vertically for 12 h. The GFP expression was monitored by a fluorescent microscope (Olympus SZX12 or Olympus BX50: Ex 460-490 nm, Em > 510 nm) and recorded with a digital camera.
17. K.D. Dittmar, D.R. Demady, L.F. Stancato, P. Krishna, and W.B. Pratt J. Biol. Chem. 272 1997 21213
18. M. Tada, M.A. O'Reilly, and J.C. Smith Development 124 1997 2225
19. G.V. Ermakova, E.M. Alexandrova, O.V. Kazanskaya, O.L. Vasiliev, M.W. Smith, and A.G. Zaraisky Development 126 1999 4513
20. T. Aoyama, C.H. Dong, Y. Wu, M. Carabelli, G. Sessa, I. Ruberti, G. Morelli, and N.H. Chua Plant Cell 7 1995 1773
21. B. Brockmann, M.W. Smith, A.G. Zaraisky, K. Harrison, K. Okada, and Y. Kamiya Plant Cell Physiol. 42 2001 942
22. H. Fukaki, Y. Nakao, Y. Okushima, A. Theologis, and M. Tasaka Plant J. 44 2005 382
23. H. Fukaki, S. Tameda, H. Masuda, and M. Tasaka Plant J. 29 2002 153
24. Q.Q. Zhu, W. Schnabel, and H. Schupp J. Photochem. 39 1987 317