A nonradioisotope, enzymatic assay for 2-deoxyglucose uptake in L6 skeletal muscle cells cultured in a 96-well microplate

Analytical Biochemistry

Volumn 351, Issue 1, 2006, Pages 139-145

  Yamamoto, Norio ^a   Sato, Takuya ^a   Kawasaki, Kengo ^a   Murosaki, Shinji ^a   Yamamoto, Yoshihiro ^a  

^a TAKEDA PHARMACEUTICAL COMPANY LIMITED   (Japan)

Author keywords

2 Deoxyglucose; Enzymatic assay; Fluorescence; Glucose uptake; L6 cells; Resazurin; Resorufin; Skeletal muscle

Indexed keywords

CELLS; CYTOLOGY; DRUG PRODUCTS; FLUORESCENCE; GLUCOSE; INSULIN;

2-DEOXYGLUCOSE; ENZYMATIC ASSAY; GLUCOSE UPTAKE; RESAZURIN; RESORUFIN; SKELETAL MUSCLE;

MUSCLE;

2 MORPHOLINO 8 PHENYLCHROMONE; ADENOSINE TRIPHOSPHATE; CYTOCHALASIN B; DEOXYGLUCOSE; DIHYDROLIPOAMIDE DEHYDROGENASE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLUCOSE TRANSPORTER; HEXOKINASE; HEXOSE; INSULIN; INSULIN SENSITIZING AGENT; NICOTINAMIDE ADENINE DINUCLEOTIDE; PIOGLITAZONE; RESAZURIN; RESORUFIN;

ANIMAL CELL; ARTICLE; CARBOHYDRATE ANALYSIS; CELL CULTURE; CONTROLLED STUDY; DOSE RESPONSE; DRUG EFFECT; ENZYME ASSAY; FLUORESCENCE; GLUCOSE TRANSPORT; INCUBATION TIME; MICROTITER PLATE ASSAY; MYOBLAST; MYOTUBE; NONHUMAN; PRIORITY JOURNAL; RAT; SKELETAL MUSCLE;

ANIMALIA;

EID: 33644943399     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.12.011     Document Type: Article

References (23)

1. S. Sasson, R. Oron, and E. Cerasi Enzymatic assay of 2-deoxyglucose 6-phosphate for assessing hexose uptake rates in cultured cells Anal. Biochem. 215 1993 309 311
2. P.S. Walker, T. Ramlal, J.A. Donovan, T.P. Doering, A. Sandra, A. Klip, and J.E. Pessin Insulin and glucose-dependent regulation of the glucose transport system in the rat L6 skeletal muscle cell line J. Biol. Chem. 264 1989 6587 6595
3. L. Sokoloff, M. Reivich, C. Kennedy, M.H. Des Rosiers, C.S. Patlak, K.D. Pettigrew, O. Sakurada, and M. Shinohara The [14C]deoxyglucose method for the measurement of local cerebral glucose utilization: theory, procedure, and normal values in the conscious and anesthetized albino rat J. Neurochem. 28 1977 897 916
4. B.B. Kahn Type 2 diabetes: when insulin secretion fails to compensate for insulin resistance Cell 92 1998 593 596
5. J.E. Pessin, and G.I. Bell Mammalian facilitative glucose transporter family: structure and molecular regulation Annu. Rev. Physiol. 54 1992 911 930
6. A. Klip, and A. Marette Acute and chronic signals controlling glucose transport in skeletal muscle J. Cell. Biochem. 48 1992 51 60
7. Y. Mitsumoto, E. Burdett, A. Grant, and A. Klip Differential expression of the GLUT1 and GLUT4 glucose transporters during differentiation of L6 muscle cells Biochem. Biophys. Res. Commun. 175 1991 652 659
8. Y. Mitsumoto, and A. Klip Development regulation of the subcellular distribution and glycosylation of GLUT1 and GLUT4 glucose transporters during myogenesis of L6 muscle cells J. Biol. Chem. 267 1992 4957 4962
9. J.K. Manchester, M.M. Chi, J.G. Carter, M.E. Pusateri, D.B. McDougal, and O.H. Lowry Measurement of 2-deoxyglucose and 2-deoxyglucose 6-phosphate in tissues Anal. Biochem. 185 1990 118 124
10. M.F. Shanahan Modulation of hexose transport in cultured skeletal muscle Mol. Cell. Endocrinol. 38 1984 171 178
11. P. Kaliman, F. Vinals, X. Testar, M. Palacin, and A. Zorzano Phosphatidylinositol 3-kinase inhibitors block differentiation of skeletal muscle cells J. Biol. Chem. 271 1996 19146 19151
12. H. Ikeda, S. Taketomi, Y. Sugiyama, Y. Shimura, T. Sohda, K. Meguro, and T. Fujita Effects of pioglitazone on glucose and lipid metabolism in normal and insulin resistant animals Arzneimittelforschung 40 1990 156 162
13. Y. Sugiyama, S. Taketomi, Y. Shimura, H. Ikeda, and T. Fujita Effects of pioglitazone on glucose and lipid metabolism in Wistar fatty rats Arzneimittelforschung 40 1990 263 267
14. P. Perret, C. Ghezzi, L. Ogier, M. Abbadi, C. Morin, J.P. Mathieu, and D. Fagret Biological studies of radiolabeled glucose analogues iodinated in positions 3, 4 or 6 Nucl. Med. Biol. 31 2004 241 250
15. A. Johannsson, D.H. Ellis, D.L. Bates, A.M. Plumb, and C.J. Stanley Enzyme amplification for immunoassays. Detection limit of one hundredth of an attomole J. Immunol. Methods 87 1986 7 11
16. S. Nakamura, K. Arimura, K. Ogawa, and T. Yagi Use of 1-methoxy-5-methylphenazinium methyl sulfate (1-methoxyPMS) in the assay of some enzymes of diagnostic importance Clin. Chim. Acta 101 1980 321 326
17. D.B. Cook, and C.H. Self Determination of one thousandth of an attomole (1 zeptomole) of alkaline phosphatase: application in an immunoassay of proinsulin Clin. Chem. 39 1993 965 971
18. C. Olive, M.E. Geroch, and H.R. Levy Glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides. Kinetic studies J. Biol. Chem. 246 1971 2047 2057
19. S.A. Summers, V.P. Yin, E.L. Whiteman, L.A. Garza, H. Cho, R.L. Tuttle, and M.J. Birnbaum Signaling pathways mediating insulin-stimulated glucose transport Ann. NY Acad. Sci. 892 1999 169 186
20. S. Yonemitsu, H. Nishimura, M. Shintani, R. Inoue, Y. Yamamoto, H. Masuzaki, Y. Ogawa, K. Hosoda, G. Inoue, T. Hayashi, and K. Nakao Troglitazone induces GLUT4 translocation in L6 myotubes Diabetes 50 2001 1093 1101
21. B. Lohrke, S.K. Shahi, B. Kruger, P. Schmidt, U. Renne, and G. Dietl Thiazolidinedione-induced activation of the transcription factor peroxisome proliferator-activated receptor gamma in cells adjacent to the murine skeletal muscle: implications for fibroblast functions Pflugers Arch. 439 2000 288 296
22. A.G. Smith, and G.E. Muscat Skeletal muscle and nuclear hormone receptors: implications for cardiovascular and metabolic disease Int. J. Biochem. Cell Biol. 37 2005 2047 2063
23. K.N. Frayn Obesity and metabolic disease: is adipose tissue the culprit? Proc. Nutr. Soc. 64 2005 7 13