Affinity capture of a biotinylated retrovirus on macroporous monolithic adsorbents: Towards a rapid single-step purification process

Biotechnology and Bioengineering

Volumn 89, Issue 7, 2005, Pages 783-787

  Williams, Sharon L ^a   Eccleston, Mark E ^a   Slater, Nigel K H ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Affinity chromatography; Biotin; Moloney Murine leukaemia virus (MoMuLV); Monolith; Streptavidin

Indexed keywords

ADSORPTION; CELL CULTURE; POLYMERIZATION; POROSITY; PURIFICATION;

CRYOPOLYMERIZATION; GLYCIDYL METHACRYLATE; MOLONEY MURINE LEUKAEMIA VIRUS (MOMULV); MONOLITHS;

VIRUSES;

ACRYLAMIDE DERIVATIVE; ADSORBENT; BIOTIN; GLYCIDYL METHACRYLATE; METHACRYLIC ACID DERIVATIVE; N,N'' METHYLENE BIS(ACRYLAMIDE); STREPTAVIDIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AQUEOUS SOLUTION; ARTICLE; BIOTINYLATION; CELL CULTURE; COLONY FORMING UNIT; GEL; HELA CELL; HUMAN; HUMAN CELL; MOLONEY LEUKEMIA ONCOVIRUS; PARTICULATE MATTER; POLYMERIZATION; POROSITY; PURIFICATION; SUPERNATANT;

ACRYLAMIDES; ADSORPTION; ANIMALS; BIOTINYLATION; CHROMATOGRAPHY, AFFINITY; CULTURE MEDIA; FEASIBILITY STUDIES; HELA CELLS; HUMANS; METHACRYLATES; MICE; MOLONEY MURINE LEUKEMIA VIRUS; NANOSTRUCTURES; NIH 3T3 CELLS; POLYMERS; RETROVIRIDAE; STREPTAVIDIN; WATER;

EID: 16444364021     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20382     Document Type: Article

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