The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme's stability and conformational transitions linked to Na+ binding

FEBS Journal

Volumn 273, Issue 1, 2006, Pages 159-169

  De Cristofaro, Raimondo ^a   Carotti, Andrea ^b   Akhavan, Sepideh ^c,d   Palla, Roberta ^c   Peyvandi, Flora ^c   Altomare, Cosimo ^b   Mannucci, Pier Mannuccio ^c  

^a CATHOLIC UNIVERSITY   (Italy)

^b UNIVERSITY OF BARI   (Italy)

^c UNIVERSITY OF MILAN   (Italy)

^d INSERM   (France)

Author keywords

Allostery; Molecular dynamics; pKa values; Stability; Thrombin

Indexed keywords

LYSINE; SODIUM; THROMBIN; UREA;

ALLOSTERISM; ARTICLE; BINDING AFFINITY; CONFORMATIONAL TRANSITION; DELETION MUTANT; DISULFIDE BOND; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME CONFORMATION; ENZYME DENATURATION; ENZYME STABILITY; ENZYME STRUCTURE; FLUORESCENCE ANALYSIS; IONIZATION; MOLECULAR DYNAMICS; MOLECULAR WEIGHT; PH MEASUREMENT; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTON TRANSPORT; SODIUM TRANSPORT; STRUCTURE ANALYSIS; CHEMISTRY; COMPARATIVE STUDY; DRUG EFFECT; GENETICS; KINETICS; METABOLISM; MUTATION; PH; PROTEIN BINDING; PROTEIN CONFORMATION; TIME;

CATALYTIC DOMAIN; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; KINETICS; LYSINE; MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; SODIUM; THROMBIN; TIME FACTORS;

EID: 33644876813     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2005.05052.x     Document Type: Article

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