Discovering new invertebrate neuropeptides using mass spectrumetry

Mass Spectrometry Reviews

Volumn 25, Issue 1, 2006, Pages 77-98

  Hummon, Amanda B ^a   Amare, Andinet ^a   Sweedler, Jonathan V ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Imaging mass spectrometry; Invertebrate neuropeptides; Post translational modifications; Sampling strategies

Indexed keywords

IMAGING MASS SPECTROMETRY; INVERTEBRATE NEUROPEPTIDES; SAMPLING STRATEGIES;

GENES; IONIZATION; LASERS; MASS SPECTROMETRY;

PROTEINS;

NEUROPEPTIDE;

ANIMAL; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; INVERTEBRATE; MASS SPECTROMETRY; PHYSIOLOGY; REVIEW;

ANIMALS; INVERTEBRATES; NEUROPEPTIDES; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 33644843132     PISSN: 02777037     EISSN: None     Source Type: Journal    
DOI: 10.1002/mas.20055     Document Type: Article

References (109)

1. Alevizos A, Karagogeos D, Weiss KR, Buck L, Koester J. 1991. R15 alpha 1 and R15 alpha 2 peptides from Aplysia: Comparison of bioactivity, distribution, and function of two peptides generated by alternative splicing. J Neurobiol 22:405-417.
2. Audsley NW, Weaver RJ. 2003. Identification of neuropeptides from brains of larval Manduca sexta and Lacanobia oleracea using MALDI-TOF mass spectrometry and post-source decay. Peptides 24:1465-1474.
3. Baggerman G, Cerstiaens A, De Loof A, Schoofs L. 2002. Peptidomics of the larval Drosophila melanogaster central nervous system. J Biol Chem 277:40368-40374.
4. Baggerman G, Clynen E, Huybrechts J, Verleyen P, Clerens S, De Loof A, Schoofs L. 2003. Peptide profiling of a single Locusla migratoria corpus cardiacum by nano-LC tandem mass spectrometry. Peptides 24:1475-1485.
5. Birgul N, Weise C, Kreienkamp HJ, Richter D. 1999. Reverse physiology in Drosophila: Identification of a novel allatostatin-like neuropeptide and its cognate receptor structurally related to the mammalian somatostatin/ galanin/opioid receptor family. EMBO J 18:5892-5900.
6. Caprioli RM, Farmer TB, Gile J. 1997. Molecular imaging of biological samples: Localization of peptides and proteins using MALDI-TOF MS. Anal Chem 69:4751-4760.
7. Chaurand P, Schwartz SA, Caprioli RM. 2004b. Profiling and imaging proteins in tissue sections by MS. Anal Chem 76:86A-93A.
8. Chaurand P, Caprioli RM. 2002. Direct profiling and imaging of peptides and proteins from mammalian cells and tissue sections by mass spectrometry. Electrophoresis 23:3125-3135.
9. Chaurand P, Fouchecourt S, DaGue BB, Xu BJ, Reyzer ML, Orgebin-Crist MC, Caprioli RM. 2003. Profiling and imaging proteins in the mouse epididymis by imaging mass spectrometry. Proteomics 3:2221-2239.
10. Chaurand P, Schwartz SA, Billheimer D, Xu BJ, Crecelius A, Caprioli RM. 2004a. Integrating histology and imaging mass spectrometry. Anal Chem 76:1145-1155.
11. Che FY, Yan L, Li H, Mzhavia N, Devi LA, Fricker LD. 2001. Identification of peptides from brain and pituitary of Cpe(fat)/Cpe(fat) mice. Proc Natl Acad Sci USA 98:9971-9916.
12. Christie AE, Lundquist CT, Nassel DR, Nusbaum MP. 1997. Two novel tachykinin-related peptides from the nervous system of the crab Cancer borealis. J Exp Biol 200:2279-2294.
13. Clynen E, Baggerman G, Veelaert D, Cerstiaens A, Van der Horst D, Harthoorn L, Denia R, Waelkens E, De Loof A, Schoofs L. 2001. Peptidomics of the pars intercerebralis-corpus cardiacum complex of the migratory locust, Locusta migratoria. Eur J Biochem 268:1929-1939.
14. Clynen E, Baggerman G, Huybrechts J, Vanden Bosch L, De Loof A, Schoofs L. 2003a. Peptidomics of the locust Corpora allata: Identification of novel pyrokinins (-FXPRLamides). Peptides 24:1493-1500.
15. Clynen E, Huybrechts J, De Loof A, Schoofs L. 2003b. Mass spectrometric analysis of the perisympathetic organs in locusts: Identification of novel periviscerokinins. Biochem Biophys Res Commun 300:422-428.
16. Coast GM, Rayne RC, Hayes TK, Mallet AI, Thompson KS, Bacon JP. 1993. A comparison of the effects of two putative diuretic hormones from Locusta migratoria on isolated locust malpighian tubules. J Exp Biol 175:1-14.
17. Craig AG, Fischer WH, Park M, Rivier JE, Musselman BD, Powell JF, Reska-Skinner SM, Prakash MO, Mackie GO, Sherwood NM. 1997. Sequence of two gonadotropin releasing hormones from tunicate suggest an important role of conformation in receptor activation. FEBS Lett 413: 215-225.
18. de With ND, Li KW, Jimenez CR, Vonk N, Dreisewerd K, Hillenkamp F, Karas M, Geraerts WP. 1997. Intracellular degradation of C-peptides in molluscan neurons producing insulin-related hormones. Peptides 18: 765-770.
19. Dircksen H, Burdzik S, Sauter A, Keller R. 2000. Two orcokinins and the novel octapeptide orcomyotropin in the hindgut of the crayfish Orconectes limosus: Identified myostimulatory neuropeptides originating together in neurones of the terminal abdominal ganglion. J Exp Biol 203:2807-2818.
20. Dircksen H, Bocking D, Heyn U, Mandel C, Chung JS, Baggerman G, Verhaert P, Daufeldt S, Plosch T, Jaros PP. Waelkens E, Keller R, Webster SG. 2001. Crustacean hyperglycaemic hormone (CHH)-like peptides and CHH-precursor-related peptides from pericardial organ neurosecretory cells in the shore crab, Carcinus maenas, are putatively spliced and modified products of multiple genes. Biochem J 356:159-170.
21. Duve H, Johnsen AH, Scott AG, Thorpe A. 2001. Allatostatins of the tiger prawn, Penaeus monodon (Crustacea: Penaeidea). Peptides 23:1039-1051.
22. Floyd PD, Li L, Moroz TP, S weedier JV. 1999. Characterization of peptides from Aplysia using microbore liquid chromatography with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry guided purification. J Chromatogr A 830:105-113.
23. Floyd PD, Li L, Rubakhin SS, Sweedler JV, Horn CC, Kupfermann I, Alexeeva V, Ellis TA, Dembrow NC, Weiss KR, Vilim FS. 1999. Aplysia calif arnica insulin prohormone processing, distribution, and relation to metabolism. J Neurosci 19:7732-7741.
24. Fujisawa Y, Furukawa Y, Ohta S, Ellis TA, Dembrow NC, Li L, Floyd PD, Sweedler JV, Minakata H, Nakamaru K, Morishita F, Matsushima O, Weiss KR, Vilim FS. 1999. The Aplysia MIP-related peptides (AMRPs): Identification, cloning, processing, distribution, and action. J Neurosci 19:9618-9634.
25. Furuya K, Hackett M, Cirelli MA, Schegg KM, Wang H, Shabanowitz J, Hunt DF, Schooley DA. 1999. A cardioactive peptide from the southern armyworm, Spodoptera eridania. Peptides 20:53-61.
26. Garden R, Moroz L, Moroz T, Shippy S, Sweedler J. 1996. Excess salt removal with matrix rinsing: Direct peptide profiling of neurons from marine invertebrates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Mass Spectrom 10:1126-1130.
27. Garden RW, Shippy SA, Li L, Moroz TP, Sweedler JV. 1998. Proteolytic processing of the Aplysia egg-laying hormone prohormone. Proc Natl Acad Sci USA 95:3972-3977.
28. Garden RW, Moroz TP, Gleeson JM, Floyd PD, Li L, Rubakhin SS, Sweedler JV. 1999. Formation of N-pyroglutamyl peptides from N-Glu and N-Gln precursors in Aplysia neurons. J Neurochem 72:676-681.
29. Greve P, Sorokine O, Berges T, Lacombe C, Van Dorsselaer A, Martin G. 1999. Isolation and amino acid sequence of a peptide with vitellogenesis inhibiting activity from the terrestrial isopod Armadillidium vulgare (Crustacea). Gen Comp Endocrinol 115:406-414.
30. Gusev AI, Vasseur OJ, Proctor A, Sharkey AG, Hercules DM. 1995. Imaging of thin-layer chromatograms using matrix-assisted laser desorption/ ionization mass spectrometry. Anal Chem 67:4565-4570.
31. Hua Y, Ishibashi J, Saito H, Tawfik A, Sakakibara M, Tanaka Y, Derua R, Waelkens E, Baggerman G, De Loof A, Schoofs L, Tanaka S. 2000. Identification of [Arg7] corazonin in the silkworm, Bombyx mori and the cricket, Gryllus bimaculatus, as a factor inducing dark color in an albino strain of the locust, Locusta migratoria. J Insect Physiol 46: 853-860.
32. Huybrechts J, Clynen E, Baggerman G, De Loof A, Schoofs L. 2002. Isolation and identification of the AKH III precursor-related peptide from Locusta migratoria. Biochem Biophys Res Commun 296:1112-1117.
33. Huybrechts J, Nusbaum MP, Bosch LV, Baggerman G, De Loof A, Schoofs L. 2003. Neuropeptidomic analysis of the brain and thoracic ganglion from the Jonah crab. Cancer borealis. Biochem Biophys Res Commun 308: 535-544.
34. Jimenez CR, Li KW, Dreisewerd K, Spijker S, Kingston R, Bateman RH, Burlingame AL, Smit AB, van Minnen J, Gerearts WP. 1998. Direct mass spectrometric peptide profiling and sequencing of single neurons reveals differential peptide patterns in a small neuronal network. Biochemistry 37:2070-2076.
35. Johnsen AH, Duve H, Davey M, Hall M, Thorpe A. 2000. Sulfakinin neuropeptides in a crustacean. Isolation, identification and tissue localization in the tiger prawn Penaeus monodon. Eur J Biochem 267:1153-1160.
36. Juhasz P, Campbell JM, Vestal ML. 2002. MALDI-TOF/TOF technology for peptide sequencing and protein identification. In: Silberring J, Ekman R, editors. Mass Spectroraetry and Hyphenated Techniques in Neuropeptide Research. John Wiley & Sons, Inc. p 375-413.
37. Kaldany RJ, Campanelli JT, Makk G, J. EC SRH. 1986. Proteolytic processing of a peptide precursor in Aplysia neuron R14. J Biol Chem 261:5751-5757.
38. Karas M, Hillenkamp F. 1988. Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. Anal Chem 60:2299-2301.
39. Kellersberger KA, Tan PV, Laiko VV, Doroshenko VM, Fabris D. 2004. Atmospheric pressure MALDI-Fourier transform mass spectrometry. Anal Chem 76:3930-3934.
40. Kruse R, Sweedler JV. 2003. Spatial profiling invertebrate ganglia using MALDI MS. J Am Soc Mass Spectrom 14:752-759.
41. Kutz KK, Schmidt JJ, Li L. 2004. In situ tissue analysis of neuropeptides by MALDI FTMS in-cell accumulation. Anal Chem 76:5630-5640.
42. Laugesen S, Roepstorff P. 2003. Combination of two matrices results in improved performance of MALDI MS for peptide mass mapping and protein analysis. J Am Soc Mass Spectrom 14:992-1002.
43. Li L, Garden RW, Sweedler JV. 2000a. Single-cell MALDI: A new tool for direct peptide profiling. Trends Biotechnol 18:151-160.
44. Li L, Moroz TP, Garden RW, Floyd PD, Weiss KR, Sweedler JV. 1998. Mass spectrometric survey of interganglionically transported peptides in Aplysia. Peptides 19:1425-1433.
45. Li L, Romanova EV, Rubakhin SS, Alexeeva V, Weiss KR, Vilim FS, Sweedler JV. 2000b. Peptide profiling of cells with multiple gene products: Combining immunochemistry and MALDI mass spectrometry with on-plate microextraction. Anal Chem 72:3867-3874.
46. Li L, Pulver SR, Kelley WP, Thirumalai V, Sweedler JV, Marder E. 2002. Orcokinin peptides in developing and adult crustacean stomatogastric nervous systems and pericardial organs. J Comp Neurol 444:227-244.
47. Li L, Kelley WP, Billimoria CP, Christie AE, Pulver SR, Sweedler JV, Marder E. 2003. Mass spectrometric investigation of the neuropeptide complement and release in the pericardial organs of the crab. Cancer borealis. J Neurochem 87:642-656.
48. Lorenz MW, Kellner R, Hoffmann KH, Gade G. 2000. Identification of multiple peptides homologous to cockroach and cricket allatostatins in the stick insect Carausius morosus. Insect Biochem Mol Biol 30: 711-718.
49. Ma PW, Garden RW, Niermann JT, O'Connor M, Sweedler JV, Roelofs WL. 2000. Characterizing the Hez-PBAN gene products in neuronal clusters with immunocytochemistry and MALDI MS. J Insect Physiol 46:221-230.
50. Marks NJ, Maule AG, Geary TG, Thompson DP, Li C, Halton DW, Shaw C. 1998. KSAYMRFamide (PF3/AF8) is present in the free-living nematode, Caenorhabditis elegans. Biochem Biophys Res Commun 248:422-425.
51. Marks NJ, Sangster NC, Maule AG, Halton DW, Thompson DP, Geary TG, Shaw C. 1999. Structural characterisation and pharmacology of KHEYLRFamide (AF2) and KSAYMRFamide (PF3/AF8) from Haemonchus contortus. Mol Biochem Parasitai 100:185-194.
52. Marks NJ, Shaw C, Halton DW, Thompson DP, Geary TG, Li C, Maule AG. 2001. Isolation and preliminary biological assessment of AADGA-PLIRFamide and SVPGVLRFamide from Caenorhabditis elegans. Biochem Biophys Res Commun 286:1170-1176.
53. Meola SM, Clottens FL, Holman GM, Nachman RJ, Nichols R, Schoofs L, Wright MS, Olson JK, Hayes TK, Pendleton MW. 1998. Isolation and immunocytochemical characterization of three tachykinin-related peptides from the mosquito, Culex salinarius. Neurochem Res 23: 189-202.
54. Miller MW, Beushausen S, Cropper E, Eisinger K, Stamm S, Vilim FS, Vitek A, Zajc A, Kupfermann I, Brosius J. 1993. The buccalin-related neuropeptides: Isolation and characterization of an Aplysia cDNA clone encoding a family of peptide cotransmitters. J Neurosci 13:3346-3357.
55. Moosler A, Rinehart KL, Grimmelikhuijzen CJ. 1996. Isolation of four novel neuropeptides, the hydra-RFamides I-IV, from Hydra magnipapillata. Biochem Biophys Res Commun 229:596-602.
56. Moosler A, Rinehart KL, Grimmelikhuijzen CJ. 1997. Isolation of three novel neuropeptides, the Cyanea-RFamides I-III, from Scyphomedusae. Biochem Biophys Res Commun 236:743-749.
57. Nachman RJ, Holman CM, Cook BJ, Haddon WF, Ling N. 1986a. Leucosulfakinin-II, a blocked sulfated insect neuropeptide with homology to cholecystokinin and gastrin. Biochem Biophys Res Commun 140:357-364.
58. Nachman RJ, Holman GM, Haddon WF, Ling N. 1986b. Leucosulfakinin, a sulfated insect neuropeptide with homology to gastrin and cholecystokinin. Science 234:71-73.
59. Nassel DR, Persson MG, Muren JE. 2000. Baratin, a nonamidated neurostimulating neuropeptide, isolated from cockroach brain: Distribution and actions in the cockroach and locust nervous systems. J Comp Neurol 422:267-286.
60. Page JS, Rubakhin SS, Sweedler JV. 2002. Single-neuron analysis using CE combined with MALDI MS and radionuclide detection. Anal Chem 74:497-503.
61. Perry SJ, Dobbins AC, Schofield MG, Piper MR, Benjamin PR. 1999. Small cardioactive peptide gene: Structure, expression and mass spectrometric analysis reveals a complex pattern of co-transmitters in a snail feeding neuron. Eur J Neurosci 11:655-662.
62. Predel R. 2001. Peptidergic neurohemal system of an insect: Mass spectrometric morphology. J Comp Neurol 436:363-375.
63. Predel R, Eckert M, Holman GM. 1999. The unique neuropeptide pattern in abdominal perisympathetic organs of insects. Ann NY Acad Sci 897: 282-290.
64. Predel R, Eckert M. 2000. Tagma-specific distribution of FXPRLamides in the nervous system of the American cockroach. J Comp Neurol 419: 352-363.
65. Predel R, Gade G. 2002. Identification of the abundant neuropeptide from abdominal perisympathetic organs of locusts. Peptides 23:621-627.
66. Predel R, Kellner R, Kaufmann R, Penzlin H, Gade G. 1997. Isolation and structural elucidation of two pyrokinins from the retrocerebral complex of the American cockroach. Reg Peptides 71:199-205.
67. Predel R, Brandt W, Kellner R, Rapus J, Nachman RJ, Gade G. 1999a. Posttranslational modifications of the insect sulfakinins: Sulfation, pyroglutamate-formation and O-methylation of glutamic acid. Eur J Biochem 263:552-560.
68. Predel R, Kellner R, Nachman RJ, Holman GM, Rapus J, Gade G. 1999b. Differential distribution of pyrokinin-isoforms in cerebral and abdominal neurohemal organs of the American cockroach. Insect Biochem Mol Biol 29:139-144.
69. Predel R, Kellner R, Rapus J, Gade G. 1999c. Allatostatins from the retrocerebral complex and antennal pulsatile organ of the American cockroach: Structural elucidation by matrix-assisted laser desorption/ ionization-time-of- flight mass spectrometry. Reg Peptides 82:81-89.
70. Predel R, Kellner R, Baggerman G, Steinmetzer T, Schoofs L. 2000. Identification of novel periviscerokinins from single neurohaemal release sites in insects MS/MS fragmentation complemented by Edman degradation. Eur J Biochem 267:3869-3873.
71. Predel R, WegenerC, Russell WK, Tichy SE, Russell DH, Nachman RJ. 2004. Peptidomics of CNS-associated neurohemal systems of adult Drosophila melanogaster. A mass spectrometric survey of peptides from individual flies. J Comp Neurol 474:379-392.
72. Predel R, Neupert S, Wicher D, Gundel M, Roth S, Derst C. 2004a. Unique accumulation of neuropeptides in an insect: FMRFamide-related peptides in the cockroach, Periplaneta americana. Eur J Neurosci 20:1499-1513.
73. Predel R, Wegener C, Russell WK, Tichy SE, Russell DH, Nachman RJ. 2004b. Peptidomics of CNS-associated neurohemal systems of adult Drosophila melanogaster. A mass spectrometric survey of peptides from individual flies. J Comp Neurol 474:379-392.
74. Rubakhin SS, Greenough WT, Sweedler JV. 2003. Spatial profiling with MALDI MS: Distribution of neuropeptides within single neurons. Anal Chem 75:5374-5380.
75. Rubakhin SS, Li L, Moroz TP, Sweedler JV. 1999. Characterization of the Aplysia californica cerebral ganglion F cluster. J Neurophysiol 81: 1251-1260.
76. Rubakhin SS, Garden RW, Fuller RR, Sweedler JV. 2000. Measuring the peptides in individual organelles with mass spectrometry. Nature Biotech 18:172-175.
77. Salzet M, Vandenbulcke F, Verger-Bocquet M. 1996. Structural characterization of osmoregulator peptides from the brain of the leech Theromyzon tessulatum: IPEPYVWD and IPEPYVWD-amide. Brain Res Mol Brain Res43:301-310.
78. Salzet M, Stefano G. 1997. Prodynorphin in invertebrates. Brain Res Mol Brain Res 52:46-52.
79. Salzet M, Wattez C, Bulet P, Malecha J. 1994. Isolation and structural characterization of a novel peptide related to gamma-melanocyte stimulating hormone from the brain of the leech Themmyzon tessulatum. FEBS Lett 348:102-106.
80. Salzet M, Bulet P, Verger-Bocquet M, Malecha J. 1995a. Isolation and structural characterization of enkephalins in the brain of the rhyn-chobdellid leech Theromyzon tessulatum. FEBS Lett 357:187-191.
81. Salzet M, Bulet P, Wattez C, Verger-Bocquet M, Malecha J. 1995b. Structural characterization of a diuretic peptide from the central nervous system of the leech Erpobdella octoculata. Angiotensin II amide. J Biol Chem 270:1575-1582.
82. Schoofs L, Baggerman G, Veelaert D, Breuer M, Tanaka S, De Loof A. 2000. The pigmentotropic hormone [His(7)]-corazonin, absent in a Locusta migratoria albino strain, occurs in an albino strain of Schistocerca gregaria. Mol Cell Endocrinol 168:101-109.
83. Scott JR, Schuerch S, Moore S, Wilkins CL. 1997. Evaluation of MALDI-FTMS for analysis of peptide mixtures generated by ladder sequencing. Int J Mass Spectrom Ion Processes 160:291-302.
84. Skold K, Svensson M, Kaplan A, Bjorkesten L, Astrom J, Andren PE. 2002. A neuroproteomic approach to targeting neuropeptides in the brain. Proteomics 2:447-454.
85. Smit AB, van Kesteren RE, Spijker S, Van Minnen J, van Golen FA, Jimenez CR, Li KW. 2003. Peptidergic modulation of male sexual behavior in Lymnaea stagnalis: Structural and functional characterization of -FVamide neuropeptides. J Neurochem 87:1245-1254.
86. Spengler B, Hubert M. 2002. Scanning microprobe matrix-assisted laser desorption ionization (SMALDI) mass spectrometry: Instrumentation for sub-micrometer resolved LDI and MALDI surface analysis. J Am Soc Mass Spectrom 13:735-748.
87. Spittaels K, Vankeerberghen A, Torrekens S, Devreese B, Grauwels L, Van Leuven F, Hunt D, Shabanowitz J, Schoofs L, Van Beeumen J, De Loof A. 1995. Isolation of Alal-proctolin, the first natural analogue of proctolin, from the brain of the Colorado potato beetle. Mol Cell Endocrinol 110:119-124.
88. Stoeckli M, Chaurand P, Hallahan DE, Caprioli RM. 2001. Imaging mass spectrometry: A new technology for the analysis of protein expression in mammalian tissues. Nat Med 7:493-496.
89. Svensson M, Skold K, Svenningsson P, Andren PE. 2003. Peptidomics-based discovery of novel neuropeptides. J Proteome Res 2:213-219.
90. Sweedler JV, Li L, Rubakhin SS, Alexeeva V, Dembrow NC, Dowling O, Jing J. Weiss KR, Vilim FS. 2002. Identification and characterization of the feeding circuit-activating peptides, a novel neuropeptide family of Aplysia. J Neurosci 22:7797-7808.
91. Taub-Montemayor TE, Linse KD, Rankin MA. 1997. Isolation and characterization of Melanoplus sanguinipes adipokinetic hormone: A new member of the AKH/RPCH family. Biochem Biophys Res Commun 239:763-768.
92. Taussig R, Scheller RH. 1986. The Aplysia FMRFamide gene encodes sequences related to mammalian brain peptides. DNA 5:453-461.
93. Torfs P, Nieto J, Cerstiaens A, Boon D, Baggerman G, Poulos C, Waelkens E, Derua R, Calderon J, De Loof A, Schoofs L. 2001. Pyrokinin neuropeptides in a crustacean. Isolation and identification in the white shrimp Penaeus vannamei. Eur J Biochem 268:149-154.
94. Torfs P, Baggerman G, Meeusen T, Nieto J, Nachman RJ, Calderon J, De Loof A, Schoofs L. 2002. Isolation, identification, and synthesis of a disulfated sulfakinin from the central nervous system of an arthropod the white shrimp Litopenaeus vannamei. Biochem Biophys Res Commun 299:312-320.
95. van Veelen PA, Jimenez CR, Li KW. Wildering WC, Gerearts WP, Tjaden UR, van der Greef J. 1993. Direct peptide profiling of single neurons by matrix-assisted laser desorption-ionization mass spectrometry. Organic Mass Spectrom 28:1542-1546.
96. Veelaert D, Baggerman G, Derua R, Waelkens E, Meeusen T, Vande Water G, De Loof A, Schoofs L. 1999. Identification of a new tachykinin from the midgut of the desert locust, Schistocerca gregaria, by ESI-Qq-oa-TOF mass spectrometry. Biochem Biophys Res Commun 266:237-242.
97. Verleyen P, Huybrechts J, Sas F, Clynen E, Baggerman G, De Loof A, Schoofs L. 2004. Neuropeptidomics of the grey flesh fly, Neobellieria buttata. Biochem Biophys Res Commun 316:763-770.
98. Verleyen P, Baggerman G, Wiehart U, Schoeters E, Van Lommel A, De Loof A, Schoofs L. 2004a. Expression of a novel neuropeptide, NVGTLARDFQLPIPNamide, in the larval and adult brain of Drosophila melanogasler. J Neurochem 88:311-319.
99. Verleyen P, Huybrechts J, Baggerman G, Van Lommel A, De Loof A, Schoofs L. 2004b. SIFamide is a highly conserved neuropeptide: A comparative study in different insect species. Biochem Biophys Res Commun 320:334-341.
100. Wainwright G. Webster SG, Wilkinson MC, Chung JS, Rees HH. 1996. Structure and significance of mandibular organ-inhibiting hormone in the crab, Cancerpagurus. Involvement in multihormonal regulation of growth and reproduction. J Biol Chem 271:12749-12754.
101. Wegener C, Predel R, Eckert M. 1999. Quantification of periviscerokinin-1 in the nervous system of the American cockroach, Periplaneta americana. An insect neuropeptide with unusual distribution. Arch Insect Biochem Physiol 40:203-211.
102. Winther AM, Siviter RJ, Isaac RE, Predel R, Nassel DR. 2003. Neuronal expression of tachykinin-related peptides and gene transcript during postembryonic development of Drosophila. J Comp Neurol 464: 180-196.
103. Woodhead AP, Thompson ME, Chan KK, Stay B. 2003. Allatostatin in ovaries, oviducts, and young embryos in the cockroach Diploptera punctata. J Insect Physiol 49:1103-1114.
104. Worster BM, Yeoman MS, Benjamin PR. 1998. Matrix-assisted laser desorption/ionization time of flight mass spectrometric analysis of the pattern of peptide expression in single neurons resulting from alternative splicing of the FMRFamide gene. Eur J Biochem 10:3498-3507.
105. Yasuda A, Yasuda-Kamatani Y, Nozaki M. Nakajima T. 2004. Identification of GYRKPPFNGSIFamide (crustacean-SIFamide) in the crayfish Procambarus clarkii by topological mass spectrometry analysis. Gen Comp Endocrinol 135:391-400.
106. Yasuda-Kamatani Y, Yasuda A. 2000. Identification of orcokinin gene-related peptides in the brain of the crayfish Procambarus clarkii by the combination of MALDI-TOF and on-line capillary HPLC/Q-Tof mass spectrometries and molecular cloning. Gen Comp Endocrinol 118: 161-172.
107. Yasuda-Kamatani Y, Yasuda A. 2004. APSGFLGMRamide is a unique tachykinin-related peptide in crustaceans. Eur J Biochem 271:1546-1556.
108. Yew JY, Dikler S, Stretton AO. 2003. De novo sequencing of novel neuropeptides directly from Ascaris suum tissue using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight. Rapid Commun Mass Spectrom 17:2693-2698.
109. Zatylny C, Gagnon J, Boucaud-Camou E, Henry J. 2000. The SepOvotropin: A new ovarian peptide regulating oocyte transport in Sepia officinalis. Biochem Biophys Res Commun 276:1013-1018.