Determination of processed animal proteins in feed: The performance characteristics of classical microscopy and immunoassay methods

Food Additives and Contaminants

Volumn 23, Issue 3, 2006, Pages 252-264

  von Holst, Christoph ^a   Boix, A ^a   Baeten, V ^b   Vancutsem, J ^c   Berben, G ^b  

^a JOINT RESEARCH CENTRE   (Italy)

^b WALLOON AGRICULTURAL RESEARCH CENTRE   (Belgium)

^c Federal Agency for the Security of the Food Chain (AFSCA FAVV)   (Belgium)

Author keywords

Animal feed; Bovine spongiform encephalopathy; Immunoassays; Meat and bone meal (MBM); Microscopy; Processed animal proteins (PAPs); Species identification

Indexed keywords

ANIMAL FOOD; ANIMAL PRODUCT; ARTICLE; BOVIDS; CONTROLLED STUDY; EUROPE; FOOD CONTAMINATION; FOOD PROCESSING; FOOD SAFETY; IMMUNOASSAY; LAW; MEAT; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; SPECIES DIFFERENCE; SPECIES IDENTIFICATION;

ANIMALS; CALCIUM PHOSPHATES; CATTLE; DIETARY PROTEINS; FEATHERS; FISH PRODUCTS; IMMUNOASSAY; MEAT; MICROSCOPY; MINERALS; SPECIES SPECIFICITY; SWINE;

ANIMALIA; BOVIDAE; BOVINAE;

EID: 33644827118     PISSN: 0265203X     EISSN: 14645122     Source Type: Journal    
DOI: 10.1080/02652030500471804     Document Type: Article

References (19)

1. Baeten, V and von Holst, C and Garrido, A and Vancutsem, J and Michotte Renier, A and Dardenne, P. (2005) Detection of banned meat and bone meal in feedstuffs by near-infrared microscopy analysis of the dense sediment fraction Analytical and Bioanalytical Chemistry, 382, pp. 149 - 157.
2. Commission Directive EC/88/1998.Commission Directive establishing guidelines for microscopic identification and estimation of constituents of animal origin for the official control of feedingstuffs Official Journal of the European Communities 27.11.98, L318, pp. 45 - 50.
3. Commission Directive EC/2003/126.Commission Directive of 23 December 2003 on the analytical method for the determination of constituents of animal origin for the official control of feedingstuffs Official Journal of the European Communities 24.12.2003, L339, pp. 78 - 84.
4. EC Regulation 999/2001 of the European Parliament and of the Council laying down rules for the prevention, control and eradication of certain transmissible spoongiform encephalopathies Official Journal of the European Communities. 31.5.01, L147, pp. 1 - 40.
5. EC Regulation 1774/2002 of the European Parliament and of the Council laying down health rules concerning animal by-products not intended for human consumption Official Journal of the European Communities, L273, pp. 1 - 95. 10.10.2002
6. EC Regulation 1234/2003 amending Annexes I. IV and XI to EC Regulation 999/2001 Official Journal of the European Communities, L173. 11.07.2003
7. European Commission.(2005). European Commission, Direction General for Health and Consumer Protection, Summary minutes of the meeting of the Standing Committee on the food chain and animal health, animal nutrition section. BrusselsApril27-282005. In [pp.].
8. Available from:
9. Gizzi, G and van Raamsdonk, LWD and Baeten, V and Murray, I and Berben, G and Brambilla, G and von Holst, C. (2003) An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongiform encephalopathy Scientific and Technical Review of the Office International des Epizooties, 22(1), pp. 311 - 331.
10. Gizzi, G and von Holst, C and Baeten, V and Berben, G and van Raamsdonk, L. (2004) Determination of processed animal proteins, including meat and bone meal, in animal feed Journal of AOAC International, 87, pp. 1334 - 1341.
11. Holst von, C and van Raamsdonk, LWD and Baeten, V and Strathmann, S and Boix, A.(2005) The validation of the microscopic method selected in the Stratfeed project for the detection of processed animal proteins. In Strategies and methods to detect and quantify tissues in feedingstuffs. (pp. 156). Luxembourg: European Commission, Publication Office. ISBN 92-894-7356-8
12. Langton, SD and Chevennement, R and Nagelkerke, N and Lombard, B. (2002) Analysing collaborative trials for qualitative microbiological methods: Accordance and concordance International Journal of Food Microbiology, 79, pp. 175 - 181.
13. Michard, J and Ziebal, R. (1999) Note concernant le protocole de détection des farines de viande, d'os et de poisson dans les aliments pour animaux Annales des Falsifications et de L'expertise Chimique et Toxicologique., 947, pp. 209 - 223.
14. Pallaroni, L and Björklund, E and von Holst, C and Unglaub, W. (2001) Determination of rendering plant sterilisation conditions using a commercially available ELISA test kit developed for detection of cooked beef Journal of AOAC International, 84, pp. 1884 - 1890.
15. Statement of the Scientific Steering Committee (SSC) on its Report and Scientific Opinion on mammalian derived meat and bone meal forming a cross-contaminant of animal feedstuffs September24-251998. In [pp.]. adopted at the SSC meeting of 26-27 October 2000
16. Available from:
17. STRATFEED EC project 2001. Strategies and methods to detect and quantify mammalian tissues in feedingstuffs. In [pp.]. G6RD-2000-CT00414 (2001-2004)
18. Available from:
19. van Raamsdonk, LWD and van der Voet, H. A ring trial for the detection of animal tissues in feeds in the presence of fishmeal. Wageningen2003. In Report 2003.012 [pp.]. pp 17 with 6 addenda