Fine tuning of rabbit equilibrative nucleoside transporter activity by an alternatively spliced variant

Journal of Drug Targeting

Volumn 13, Issue 8-9, 2005, Pages 521-533

  Wu, Sharon K ^a,d   Ann, David K ^a   Kim, Kwang Jin ^b   Lee, Vincent H L ^a,c  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^c CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^d Cardinal Health   (United States)

Author keywords

Alternatively spliced variant; Basolateral nucleoside transport; C terminal variant; MDCK; rbENT2

Indexed keywords

COMPLEMENTARY DNA; EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 2; EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 2A; NITROBENZYLTHIOINOSINE; NUCLEOSIDE TRANSPORTER; RNA; TRITIUM; UNCLASSIFIED DRUG; URIDINE;

ANIMAL TISSUE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL STRAIN; GENETIC TRANSFECTION; INTESTINE; KIDNEY CORTEX; KINETICS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOSIDE TRANSPORT; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN PROCESSING; RABBIT; RNA ISOLATION; TRACHEA; WILD TYPE;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CLONING, MOLECULAR; DOGS; DRUG DELIVERY SYSTEMS; EPITHELIAL CELLS; EQUILIBRATIVE-NUCLEOSIDE TRANSPORTER 2; GENE EXPRESSION REGULATION; HUMANS; MALE; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; RABBITS; RNA, MESSENGER; SEQUENCE HOMOLOGY, AMINO ACID; THIOINOSINE; TISSUE DISTRIBUTION; TRACHEA; TRITIUM; URIDINE; VARIATION (GENETICS);

EID: 33644809830     PISSN: 1061186X     EISSN: 10292330     Source Type: Journal    
DOI: 10.1080/10611860500403099     Document Type: Article

References (30)

1. Apasov, SG and Koshiba, M and Chused, TM and Sitkovsky, MV. (1997) Effects of extracellular ATP and adenosine on different thymocyte subsets: Possible role of ATP-gated channels and G protein-coupled purinergic receptor J Immunol, 158, pp. 5095-5105.
2. Baldwin, SA and Yao, SY and Hyde, RJ and Ng, AM and Foppolo, S and Barnes, K and Ritzel, MW and Cass, CE and Young, JD. (2005) Functional characterization of novel human and mouse equilibrative nucleoside transporters (hENT3 and mENT3) located in intracellular membranes J Biol Chem, 280, pp. 15880-15887.
3. Boleti, HI and Coe, R and Baldwin, SA and Young, JD and Cass, CE. (1997) Molecular identification of the equilibrative NBMPR-sensitive (es) nucleoside transporter and demonstration of an equilibrative NBMPR-insensitive (ei) transport activity in human erythroleukemia (K562) cells Neuropharmacology, 36, pp. 1167-1179.
4. Cass, CE and Young, JD and Baldwin, SA and Cabrita, MA and Graham, KA and Griffiths, M and Jennings, LL and Mackey, JR and Ng, AM and Ritzel, MW. (1999) Nucleoside transporters of mammalian cells Pharm Biotechnol, 12, pp. 313-352.
5. Chiang, CW and Carter, N and Sullivan, WJ, Jr and Donald, RG and Roos, DS and Naguib, FN and el Kouni, MH and Ullman, B and Wilson, CM. (1999) The adenosine transporter of Toxoplasma gondii. Identification by insertional mutagenesis, cloning, and recombinant expression J Biol Chem, 274, pp. 35255-35261.
6. Crawford, CR and Patel, DH and Naeve, C and Belt, JA. (1998) Cloning of the human equilibrative, nitro-benzyl-mercapto-purine riboside (NBMPR)-insensitive nucleoside transporter ei by functional expression in a transport-deficient cell line J Biol Chem, 273, pp. 5288-5293.
7. Gati, WP and Paterson, AR and Belch, AR and Chlumecky, V and Larratt, LM and Mant, MJ and Turner, AR. (1998) es nucleoside transporter content of acute leukemia cells: Role in cell sensitivity to cytarabine (araC) Leuk Lymphoma, 32, pp. 45-54.
8. Griffith, DA and Jarvis, SM. (1996) Nucleoside and nucleobase transport systems of mammalian cells Biochim Biophys Acta, 1286, pp. 153-181.
9. Griffiths, M and Beaumont, N and Yao, SY and Sundaram, M and Boumah, CE and Davies, A and Kwong, FY and Coe, I and Cass, CE and Young, JD and Baldwin, SA. (1997a) Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs Nat Med, 3, pp. 89-93.
10. Griffiths, M and Yao, SY and Abidi, F and Phillips, SE and Cass, CE and Young, JD and Baldwin, SA. (1997b) Molecular cloning and characterization of a nitrobenzylthioinosine-insensitive (ei) equilibrative nucleoside transporter from human placenta Biochem J, 328, pp. 739-743.
11. Handa, M and Choi, D and Caldeiro, RM and Messing, RO and Gordon, AS and Diamond, I. (2001) I. Cloning of a novel isoform of the mouse NBMPR-sensitive equilibrative nucleoside transporter (ENT1) lacking a putative phosphorylation site Gene, 262, pp. 301-307.
12. Heidmann, DE and Szot, P and Kohen, R and Hamblin, MW. (1998) Function and distribution of three rat 5-hydroxytryptamine7 (5-HT7) receptor isoforms produced by alternative splicing Neuropharmacol, 37, pp. 1621-1632.
13. Hofmann, K and Stoffel, W. (1993) TMbase - a database of membrane spanning protein segments Biol Chem Hoppe Seyler, 374, pp. 166.
14. Jarvis, SM and Young, JD. (1986) Nucleoside transport in rat erythrocytes: Two components with differences in sensitivity to inhibition by nitrobenzylthioinosine and p-chloromercuriphenyl sulfonate J Membr Biol, 93, pp. 1-10.
15. Kim, KM and Kingsmore, SF and Han, H and Yang-Feng, TL and Godinot, N and Seldin, MF and Caron, MG and Giros, B. (1994) Cloning of the human glycine transporter type 1: Molecular and pharmacological characterization of novel isoform variants and chromosomal localization of the gene in the human and mouse genomes Mol Pharmacol, 45, pp. 608-617.
16. Kiss, A and Farah, K and Kim, J and Garriock, RJ and Drysdale, TA and Hammond, JR. (2000) Molecular cloning and functional characterization of inhibitor-sensitive (mENT1) and inhibitor-resistant (mENT2) equilibrative nucleoside transporters from mouse brain Biochem J, 352, pp. 363-372.
17. Koshiba, M and Kojima, H and Huang, S and Apasov, S and Sitkovsky, MV. (1997) Memory of extracellular adenosine A2A purinergic receptor-mediated signaling in murine T cells J Biol Chem, 272, pp. 25881-25889.
18. Mathias, NR and Kim, K-J and Lee, VHL. (1996) Targeted drug delivery to the respiratory tract: Solute permeability of air-interface cultured rabbit tracheal epithelial cell monolayers J Drug Targeting, 4, pp. 79-86.
19. Olsen, MA and Nawoschik, SP and Schurman, BR and Schmitt, HL and Burno, M and Smith, DL and Schechter, LE. (1999) Identification of a human 5-HT6 receptor variant produced by alternative splicing Brain Res Mol Brain Res, 64, pp. 255-263.
20. Osses, NJ and Pearson, D and Yudilevich, DL and Jarvis, SM. (1996) Hypoxanthine enters human vascular endothelial cells (ECV 304) via the nitrobenzylthioinosine-insensitive equilibrative nucleoside transporter Biochem J, 317, pp. 843-848.
21. Pagani, F and Buratti, E and Stuani, C and Romano, M and Zuccato, E and Niksic, M and Giglio, L and Faraguna, D and Baralle, FE. (2000) Splicing factors induce cystic fibrosis transmembrane regulator exon 9 skipping through a non-evolutionary conserved intronic element J Biol Chem, 275, pp. 21041-21047.
22. Ross, RJ and Esposito, N and Shen, XY and Von Laue, S and Chew, SL and Dobson, PR and Postel-Vinay, MC and Finidori, J. (1997) A short isoform of the human growth hormone receptor functions as a dominant negative inhibitor of the full-length receptor and generates large amounts of binding protein Mol Endocrinol, 11, pp. 265-273.
23. +/peptide co-transporter, PEPT1 Biochem Biophys Res Commun, 237, pp. 577-582.
24. Sundaram, M and Yao, SY and Ng, AM and Griffiths, M and Cass, CE and Baldwin, SA and Young, JD. (1998) Chimeric constructs between human and rat equilibrative nucleoside transporters (hENT1 and rENT1) reveal hENT1 structural domains interacting with coronary vasoactive drugs J Biol Chem, 273, pp. 21519-21525.
25. Sundaram, M and Yao, SY and Ng, AM and Cass, CE and Baldwin, SA and Young, JD. (2001) Equilibrative nucleoside transporters: Mapping regions of interaction for the substrate analogue nitrobenzylthioinosine (NBMPR) using rat chimeric proteins Biochemistry, 40, pp. 8146-8151.
26. Thompson, JD and Higgins, DG and Gibson, TJ. (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucleic Acids Res, 22, pp. 4673-4680.
27. Vasudevan, G and Carter, NS and Drew, ME and Beverley, SM and Sanchez, MA and Seyfang, A and Ullman, B and Landfear, SM. (1998) Cloning of Leishmania nucleoside transporter genes by rescue of a transport-deficient mutant Proc Natl Acad Sci USA, 95, pp. 9873-9878.
28. Wang, JM and Schaner, E and Thomassen, S and Su, SF and Piquette-Miller, M and Giacomini, KM. (1997) Functional and molecular characteristics of Na(+)-dependent nucleoside transporters Pharm Res, 14, pp. 1524-1532.
29. Wu, SK and Mathias, NR and Kim, KJ and Lee, VHL. (2005) Functional and pharmacological mechanisms of nucleoside transport across the basolateral membrane of rabbit tracheal epithelial cells Life Sci. Aug 17, [Epub ahead of print]
30. Yao SY, Ng AM, Muzyka WR, Griffiths M, Cass CE, Baldwin SA, Young JD. 1997. Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat tissues. J Biol Chem 272:28423-28430.