Heterozygous indels as useful tools in the reconstruction of DNA sequences and in the assessment of ploidy level and genomic constitution of hybrid organisms

DNA Sequence - Journal of DNA Sequencing and Mapping

Volumn 16, Issue 6, 2005, Pages 462-467

  Sousa Santos, Carla ^a   Robalo, Joana I ^a   Collares Pereira, Maria João ^b   Almada, Vitor C ^a  

^a INSTITUTO SUPERIOR DE PSICOLOGIA APLICADA   (Portugal)

^b UNIVERSITY OF LISBON   (Portugal)

Author keywords

Beta actin gene; Cyprinid fish; Double peaks; Haplotype reconstruction; Heterozygotes; Interspecific hybrids

Indexed keywords

BETA ACTIN; DNA FRAGMENT; RECOMBINANT DNA;

ARTICLE; ARTIFACT; DNA DETERMINATION; DNA SEQUENCE; FISH; GENOME; HETEROZYGOTE; HYBRID; METHODOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLOIDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; ANIMAL; CHIMERA; CYPRINIFORMES; FEMALE; GENETICS; HYBRIDIZATION; MALE; SPECIES DIFFERENCE;

CHONDROSTOMA OLIGOLEPIS; CYPRINIDAE; SQUALIUS ALBURNOIDES;

ANIMALS; BASE SEQUENCE; CHIMERA; CYPRINIDAE; DNA, RECOMBINANT; FEMALE; GENOME; HETEROZYGOTE; HYBRIDIZATION, GENETIC; MALE; PLOIDIES; SPECIES SPECIFICITY;

EID: 33644794538     PISSN: 10425179     EISSN: 10292365     Source Type: Journal    
DOI: 10.1080/10425170500356065     Document Type: Article

References (10)

1. Alves MJ, Coelho MM, Collares-Pereira MJ. 2001. Evolution in action through hybridization and polyploidy in an Iberian freshwater fish: A genetic review. Genetica 111:375-385.
2. Alves MJ, Collares-Pereira MJ, Dowling TE, Coelho MM. 2002. The genetics of maintenance of an all-male lineage in the Squalius alburnoides complex. J Fish Biol 60:649-662.
3. Bhangale TR, Rieder MJ, Livingston RJ, Nickerson DA. 2005. Comprehensive identification and characterization of diallelic insertion-deletion polymorphisms in 330 human candidate genes. Hum Mol Genet 14:59-69.
4. Collares-Pereira MJ. 1985. Cytotaxonomic studies in Iberian Cyprinids. II. Karyology of Anaecypris hispanica (Steindachner, 1866), Chondrostoma lemmingi (Steindachner, 1866), Rutilus arcasi (Steindachner, 1866) and R. macrolepidotus (Steindachner, 1866). Cytologia 50:879-890.
5. Cunha C, Coelho MM, Carmona JA, Doadrio I. 2004. Phylogeographical insights into the origins of the Squalius alburnoides complex via multiple hybridization events. Mol Ecol 13:2807-2817.
6. 1997. ABRF'97: Techniques at the genome/proteome interface-DNA sequence analysis, Available from http://www.biotech.iastate.edu/facilities/DSSF/ABRF/ default.html via the INTERNET. (Accessed 2005 August 31)
7. Lamatsch DK, Steinlein C, Schmid M, Schartl M. 2000. Noninvasive determination of genome size and ploidy level in fishes by flow cytometry: Detection of triploid Poecilia formosa. Cytometry 39:91-95.
8. Pala I, Coelho MM. 2005. Contrasting views over a hybrid complex: Between speciation and evolutionary "dead-end". Gene 347:283-294.
9. Robalo J, Sousa-Santos C, Levy A, Almada V. n.d. Molecular insights on the taxonomic position of the paternal ancestor of the Squalius alburnoides hybridogenetic complex. Mol Phylog Evol.
10. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: A laboratory manual. 2nd ed., New York: Cold Spring Harbor Laboratory Press.