Mutation of F417 but not of L418 or L420 in the lipid binding domain decreases the activity of triacylglycerol hydrolase

Journal of Lipid Research

Volumn 47, Issue 2, 2006, Pages 375-383

  Alam, Mustafa ^a   Gilham, Dean ^b   Vance, Dennis E ^a   Lehner, Richard ^a,b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Carboxylesterase; Lipase; Lipolysis; Mutagenesis

Indexed keywords

ALANINE; ESTER DERIVATIVE; LIPID; OCTAPEPTIDE; PHENYLALANYLLEUCYLASPARTYLLEUCYLISOLEUCYLALANYLASPARTYLVALINE; PROLINE; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG; 4 METHYLUMBELLIFERYL HEPTANOATE; 4 NITROPHENYL ACETATE; 4-METHYLUMBELLIFERYL HEPTANOATE; 4-NITROPHENYL ACETATE; 4-NITROPHENYL BUTYRATE; BUTYRIC ACID 4 NITROPHENYL ESTER; BUTYRIC ACID DERIVATIVE; CYSTEINE; DRUG DERIVATIVE; HYMECROMONE; IODOACETAMIDE; MERCAPTOETHANOL; NITROPHENOL; PHENYLALANINE; RECOMBINANT PROTEIN;

ACYLATION; ALPHA HELIX; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CATALYSIS; CATALYST; CHEMICAL MODIFICATION; CONTROLLED STUDY; COVALENT BOND; ENZYME ACTIVITY; LIPID METABOLISM; LIPOLYSIS; MOLECULAR INTERACTION; MOLECULAR MODEL; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PREDICTION; PRIORITY JOURNAL; PROTEIN BINDING; SOLUBILITY; ANIMAL; ARMYWORM; BINDING SITE; CELL LINE; CELL STRAIN COS1; CERCOPITHECUS; CHEMISTRY; ENZYME SPECIFICITY; GENE DELETION; GENE EXPRESSION; GENETIC TRANSFECTION; GENETICS; HUMAN; METABOLISM; POINT MUTATION; PROTEIN FOLDING; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

ACYLATION; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; BUTYRATES; CATALYSIS; CELL LINE; CERCOPITHECUS AETHIOPS; COS CELLS; CYSTEINE; GENE DELETION; GENE EXPRESSION; HUMANS; HYMECROMONE; IODOACETAMIDE; LIPASE; MERCAPTOETHANOL; MUTAGENESIS, SITE-DIRECTED; MUTATION; NITROPHENOLS; PHENYLALANINE; POINT MUTATION; PROTEIN FOLDING; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPODOPTERA; SUBSTRATE SPECIFICITY; TRANSFECTION;

EID: 33244496395     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: 10.1194/jlr.M500344-JLR200     Document Type: Article

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