Heterologous expression, purification, and characterization of human triacylglycerol hydrolase

Protein Expression and Purification

Volumn 24, Issue 1, 2002, Pages 33-42

  Alam, Mustafa ^a   Ho, Samuel ^a   Vance, Dennis E ^a   Lehner, Richard ^b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

TRIACYLGLYCEROL LIPASE;

AMINO ACID SEQUENCE; ANIMAL; ARMYWORM; ARTICLE; BACULOVIRUS; CELL LINE; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LIVER MICROSOME; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; ANIMALS; BACULOVIRIDAE; CELL LINE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; LIPASE; MICROSOMES, LIVER; MOLECULAR SEQUENCE DATA; SPODOPTERA;

ESCHERICHIA COLI; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0035996706     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1553     Document Type: Article

References (35)

1. The assembly and secretion of apolipoprotein B-containing lipoproteins
2. Contribution of cytoplasmic storage triacylglycerol to VLDL-triacylglycerol in isolated rat hepatocytes
3. The lipolysis/esterification cycle of hepatic triacylglycerol, its role in the secretion of very low-density lipoprotein and its response to hormone and sulphonylureas
4. Storage, mobilization and secretion of cytosolic triacylglycerol in hepatocyte cultures: The role of insulin
5. 6]ethanol
6. Use of cytosolic triacylglycerol hydrolysis products and of exogenous fatty acid for the synthesis of triacylglycerol secreted by cultured rat hepatocytes
7. Mobilization of triacylglycerol stores
8. Acid lipase deficiency
9. Purification and characterization of a porcine liver microsomal triacylglycerol hydrolase
10. Subcellular localization, developmental expression and characterization of a liver triacylglycerol hydrolase
11. Cloning and expression of a cDNA encoding a hepatic microsomal lipase that mobilizes stored triacylglycerol
12. Glycosylation-dependent activity of baculovirus-expressed human liver carboxylesterases: cDNA cloning and characterization of two highly similar enzyme forms
13. Molecular cloning and characterization of a human carboxylesterase gene
14. cDNA cloning and characterization of human monocyte/macrophage serine esterase-1
15. Cloning and analysis of a cDNA encoding a human liver carboxylesterase
16. Cloning and sequencing of a human liver carboxylesterase isoenzyme
17. A serine esterase released by human alveolar macrophages is closely related to liver microsomal carboxylesterases
18. Role of carboxylesterases in xenobiotic metabolism
19. Subcellular localization of non-specific carboxylesterases, acylcarnitine hydrolase, monoacylglycerol lipase and palmitoyl-CoA hydrolase in rat liver
20. The mammalian carboxylesterases: From molecules to functions
21. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
22. Baculovirus systems for the expression of human gene products
23. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
24. Molecular cloning and characterization of a novel putative carboxylesterase, present in human intestine and liver
25. The oligosaccharides of influenza virus hemagglutinin expressed in insect cells by a baculovirus vector
26. Domain structure analysis of recombinant rat hormone-sensitive lipase
27. One-step purification and characterization of human pancreatic lipase expressed in insect cells
28. Characterization of lysosomal acid lipase by site-directed mutagenesis and heterologous expression
29. Rat GP-3 is a pancreatic lipase with kinetic properties that differ from colipase-dependent pancreatic lipase
30. Purification of pancreatic cholesterol esterase expressed in recombinant baculovirus-infected Sf-9 cells
31. Biological roles of oligosaccharides: All of the theories are correct