Photo-CIDNP reveals differences in compaction of non-native states of lysozyme

Journal of the American Chemical Society

Volumn 128, Issue 6, 2006, Pages 1802-1803

  Schlörb, Christian ^a   Mensch, Sarah ^a   Richter, Christian ^a   Schwalbe, Harald ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; FLAVINE MONONUCLEOTIDE; HEN EGG WHITE LYSOZYME; LYSOZYME; QUERCETIN; SOLVENT; UNCLASSIFIED DRUG;

ARTICLE; CHEMICALLY INDUCED DYNAMIC NUCLEAR POLARIZATION; HYDROPHOBICITY; METHYLATION; MOLECULAR DYNAMICS; NITROGEN NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH; POINT MUTATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTON NUCLEAR MAGNETIC RESONANCE; SITE DIRECTED MUTAGENESIS; SOLVENT EFFECT; TEMPERATURE DEPENDENCE; WILD TYPE;

ANIMALS; CHICKENS; EGG PROTEINS; MURAMIDASE; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHOTOCHEMISTRY; PROTEIN FOLDING; TRYPTOPHAN;

EID: 33244463312     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja056757d     Document Type: Article

References (14)

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5. Quantification of solvent accessibility can be accomplished by time-resolved CIDNP: however, a semiquantitative analysis can also be performed with the data presented here, as discussed in ref 5.
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11. 3 The gene coding for W62G-all-Ala-HEWL has been generated by site-directed single-point mutation of the All-Ala-HEWL gene using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA) and appropriate forward and backward primers.
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13. 8 with 50 ms laser pulses. The CIDNP/HSQC intensity ratios have been calculated for every peak, and the highest value has been normalized to 1.0. Enors for the peak intensities have been extracted from the signal-to-noise ratio; the error bars for the CIDNP/HSQC ratios have been calculated by error propagation from the intensity errors.
14. 2 relaxation rates have been obtained by fitting peak intensities measured as a function of relaxation delay (ranging from 17.1 to 307.2 ms) using a pseudo-3D variant of the standard Bruker pulse sequence at a temperature of 293 K to a single-exponential decay. A two-parameter single-exponential decay function has been used for the fitting with SigmaPlot (Systat Software Inc.). Errors for the relaxation rates have been extracted from the fits.