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Volumn 121, Issue 27, 1999, Pages 6505-6506

Two-dimensional 15N-1H photo-CIDNP as a surface probe of native and partially structured proteins [5]

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN;

EID: 0033553993     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9907663     Document Type: Letter
Times cited : (41)

References (30)
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    • note
    • 15N) at pH 6.3, 7°C. The light pulses were 500 ms and 4 W power.
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    • note
    • 15N) ≅ 10.
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    • note
    • 15N polarizations, especially for denatured lysozyme where the signals are inherently weak. Optimal FMN and protein concentrations, for both native and denatured states, were, respectively, 0.2 mM and 1.0-1.5 mM. Experiments using lower pH (∼2) and urea concentrations (∼8 M) and lower temperatures (∼20°C) to denature lysozyme gave much weaker polarizations.


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