Probing in vivo promoter activities in Mycoplasma pneumoniae: A system for generation of single-copy reporter constructs

Applied and Environmental Microbiology

Volumn 72, Issue 2, 2006, Pages 1696-1699

  Halbedel, Sven ^a   Stülke, Jörg ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; CHROMOSOMES; GENES;

LACZ GENE; LACZ-BASED REPORTER PLASMID; MYCOPLASMA PNEUMONIAE; VIVO PROMOTER ACTIVITIES;

MICROBIOLOGY;

BETA GALACTOSIDASE;

MOLECULAR ANALYSIS;

ARTICLE; CONTROLLED STUDY; GENE FUSION; IN VIVO STUDY; LACZ GENE; MYCOPLASMA PNEUMONIAE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PROMOTER REGION; REPORTER GENE; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

BASE SEQUENCE; DNA, BACTERIAL; GENE FUSION; GENES, BACTERIAL; GENES, REPORTER; L-LACTATE DEHYDROGENASE; LAC OPERON; MYCOPLASMA PNEUMONIAE; PLASMIDS; PROMOTER REGIONS (GENETICS);

MYCOPLASMA PNEUMONIAE;

EID: 33144471122     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.72.2.1696-1699.2006     Document Type: Article

References (30)

1. Benders, G. A., B. C. Powell, and C. A. Hutchison III. 2005. Transcriptional analysis of the conserved ftsZ gene cluster in Mycoplasma genitalium and Mycoplasma pneumoniae. J. Bacteriol. 187:4542-4551.
2. Catrein, I., R. Dumke, J. Weiner III, E. Jacobs, and R. Hermann. 2004. Cross-complementation between the products of the genes P1 and ORF6 of Mycoplasma pneumoniae subtypes 1 and 2. Microbiology 150:3989-4000.
3. Chaudhry, R., N. Nisar, B. Hora, S. Reddy, and P. Malhotra. 2004. Expression and immunological characterization of carboxy terminal region of P1 adhesin protein of Mycoplasma pneumoniae. J. Clin. Microbiol. 43:321-325.
4. Dybvig, K., C. T. French, and L. L. Voelker. 2000. Construction and use of derivatives of transposon Tn4001 that function in Mycoplasma pulmonis and Mycoplasma arthritidis. J. Bacteriol. 182:4343-4347.
5. Gil, R., F. J. Silva, J. Peretó, and A. Moya. 2004. Determination of the core of a minimal bacterial gene set. Microbiol. Mol. Biol. Rev. 68:518-537.
6. Halbedel, S., C. Hames, and J. Stülke. 2004. In vivo activity of enzymatic and regulatory components of the phosphoenolpyruvate:sugar phosphotransferase system in Mycoplasma pneumoniae. J. Bacteriol. 186:7936-7943.
7. Hames, C., S. Halbedel, O. Schilling, and J. Stülke. 2005. MMR: a method for the simultaneous introduction of multiple mutations into the glpK gene of Mycoplasma pneumoniae. Appl. Environ. Microbiol. 71:4097-4100.
8. Hedreyda, C. T., K. K. Lee, and D. C. Krause. 1993. Transformation of Mycoplasma pneumoniae with Tn4001 by electroporation. Plasmid 30:170-175.
9. Himmelreich, R., H. Hilbert, H. Plagens, E. Pirkl, B.-C. Li, and R. Herrmann. 1996. Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae. Nucleic Acids Res. 24:4420-4449.
10. Hutchison, C. A., III, S. C. Peterson, S. R. Gill, R. T. Cline, O. White, C. M. Fraser, H. O. Smith, and J. C. Venter. 1999. Global transposon mutagenesis and a minimal Mycoplasma genome. Science 286:2165-2169.
11. Hyman, H. C., R. Gafny, G. Glaser, and S. Razin. 1988. Promoter of the Mycoplasma pneumoniae rRNA operon. J. Bacteriol. 170:3262-3268.
12. Inamine, J. M., S. Loechel, and P. C. Hu. 1988. Analysis of the nucleotide sequence of the P1 operon of Mycoplasma pneumoniae. Gene 73:175-183.
13. Inamine, J. M., K. C. Ho, S. Loechel, and P. C. Hu. 1990. Evidence that UGA is read as tryptophan codon rather than as a stop codon by Mycoplasma pneumoniae, Mycoplasma genitalium, and Mycoplasma gallisepticum. J. Bacteriol. 172:504-506.
14. Jacobs, E. 1997. Mycoplasma infections of the human respiratory tract. Wien. Klin. Wochenschr. 109:574-577.
15. Jaffe, J. D., H. C. Berg, and G. M. Church. 2004. Proteogenomic mapping as a complementary method to perform genome annotation. Proteomics 4:59-77.
16. Janis, C., C. Lartigue, J. Frey, H. Wróblewski, F. Thiaucourt, A. Blanchard, and P. Sirand-Pugnet. 2005. Versatile use of oriC plasmids for functional genomics of Mycoplasma capricolum subsp. capricolum. Appl. Environ. Microbiol. 71:2888-2893.
17. Knudtson, K. L., and F. C. Minion. 1994. Use of lac gene fusions in the analysis of Acholeplasma upstream gene regulatory sequences. J. Bacteriol. 176:2763-2766.
18. Lind, K. 1983. Manifestations and complications of Mycoplasma pneumoniae disease: a review. Yale J. Biol. Med. 56:461-468.
19. Martin-Verstraete, I., M. Débarbouillé, A. Klier, and G. Rapoport. 1992. Mutagenesis of the Bacillus subtilis "-12, -24" promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J. Mol. Biol. 226:85-99.
20. Miller, J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
21. Regula, J. T., B. Ueberle, G. Boguth, A. Görg, M. Schnölzer, R. Herrmann, and R. Frank. 2000. Towards a two-dimensional proteome map of Mycoplasma pneumoniae. Electrophoresis 21:3765-3780.
22. Rouch, D. A., M. E. Byrne, Y. C. Kong, and R. A. Skurray. 1987. The aac-aphD gentamicin and kanamycin resistance determinant of Tn4001 from Staphylococcus aureus: expression and nucleotide sequence analysis. J. Gen. Microbiol. 133:3039-3052.
23. Ueberle, B., R. Frank, and R. Herrmann. 2002. The proteome of the bacterium Mycoplasma pneumoniae: comparing predicted open reading frames to identified gene products. Proteomics 2:754-764.
24. Waites, K. B., and D. F. Talkington. 2004. Mycoplasma pneumoniae and its role as human pathogen. Clin. Microbiol. Rev. 17:697-728.
25. Waldo, R. H., III, P. L. Popham, C. E. Romero-Arroyo, E. A. Mothershed, K. K. Lee, and D. C. Krause. 1999. Transcriptional analysis of the hmw gene cluster of Mycoplasma pneumoniae. J. Bacteriol. 181:4978-4985.
26. Waldo, R. H., III, J. L. Jordan, and D. C. Krause. 2005. Identification and complementation of a mutation associated with loss of Mycoplasma pneumoniae virulence-specific proteins B and C. J. Bacteriol. 187:747-751.
27. Weiner, J., III, R. Herrmann, and G. F. Browning. 2000. Transcription in Mycoplasma pneumoniae. Nucleic Acids Res. 28:4488-4496.
28. Weiner, J., III, C. U. Zimmerman, H. W. H. Göhlmann, and R. Herrmann. 2003. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res. 31:6306-6320.
29. Willby, M. J., and D. C. Krause. 2002. Characterization of a Mycoplasma pneumoniae hmw3 mutant: implications for attachment organelle assembly. J. Bacteriol. 184:3061-3068.
30. Zimmerman, C.-U., and R. Herrmann. 2005. Synthesis of a small, cysteine-rich, 29 amino acids long peptide in Mycoplasma pneumoniae. FEMS Microbiol. Lett. 253:315-321 [Online.] doi:10.1016/j.femsle.2005.09.054.