Estimation of human sperm gene-specific deoxyribonucleic acid damage by real-time polymerase chain reaction analysis

Fertility and Sterility

Volumn 85, Issue 3, 2006, Pages 797-799

  San Gabriel, Maria ^a   Zhang, Xiaoyang ^a   Zini, Armand ^a,b  

^a ROYAL VICTORIA HOSPITAL   (Canada)

^b ST MARY S HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ANALYTIC METHOD; ARTICLE; CLINICAL ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; DNA DAMAGE; HUMAN; HUMAN CELL; MALE; MALE INFERTILITY; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEMEN ANALYSIS; SPERMATOZOON;

ALU ELEMENTS; CASE-CONTROL STUDIES; COMPUTER SYSTEMS; DNA DAMAGE; FEASIBILITY STUDIES; GLOBINS; HUMANS; INFERTILITY, MALE; INSULIN-LIKE GROWTH FACTOR II; MALE; POLYMERASE CHAIN REACTION; PROSPECTIVE STUDIES; SPERMATOZOA; TELOMERE;

EID: 32944477546     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fertnstert.2005.11.027     Document Type: Article

References (6)

1. C.M. Hughes, S.E. Lewis, V.J. McKelvey-Martin, W. Thompson A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay Mol Hum Reprod 2 1996 613 619
2. H. Shen, C. Ong Detection of oxidative DNA damage in human sperm and its association with sperm function and male infertility Free Radic Biol Med 28 2000 529 536
3. A. Zini, R. Bielecki, D. Phang, M.T. Zenzes Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men Fertil Steril 75 2001 674 677
4. World Health Organization Laboratory manual for the examination of human semen and sperm-cervical mucus interaction 4th ed. 1999 Cambridge University Press Cambridge
5. S.M. Wykes, S.A. Krawetz The structural organization of sperm chromatin J Biol Chem 278 2003 29471 29477
6. J.A. Sikorsky, D.A. Primerino, T.W. Fenger, J. Denvir Effect of DNA damage on PCR amplification with the relative threshold cycle method Biochem Biophys Res Comm 323 2004 823 830