Coupling of MBP fusion protein cleavage with sparse matrix crystallization screens to overcome problematic protein solubility

BioTechniques

Volumn 39, Issue 4, 2005, Pages 476-478

  Gruswitz, Franz ^a   Frishman, Mary ^a   Goldstein, Barry M ^a   Wedekind, Joseph E ^a  

^a UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; INOSINATE DEHYDROGENASE; MALTOSE BINDING PROTEIN; PROTEINASE; CARRIER PROTEIN; IMPDH2 PROTEIN, HUMAN; MALTOSE-BINDING PROTEIN;

ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; CRYSTALLIZATION; GENETIC LINKAGE; HUMAN; NUCLEAR MAGNETIC RESONANCE; PROTEIN EXPRESSION; SOLUBILITY; X RAY CRYSTALLOGRAPHY; X RAY DIFFRACTION; BIOTECHNOLOGY; CHEMISTRY; COMPUTER PROGRAM; INSTRUMENTATION; METHODOLOGY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN TERTIARY STRUCTURE;

BIOTECHNOLOGY; CARRIER PROTEINS; CRYSTALLOGRAPHY, X-RAY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; HYDROGEN-ION CONCENTRATION; IMP DEHYDROGENASE; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SOFTWARE; SOLUBILITY;

EID: 32944458587     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112033     Document Type: Article

References (13)

1. Kapust, R.B. and D.S. Waugh. 1999. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 8:1668-1674.
2. Smyth, D.R., M.K. Mrozkiewicz, W.J. McGrath, P. Listwan, and B. Kobe. 2003. Crystal structures of fusion proteins with large-affinity tags. Protein Sci. 12:1313-1322.
3. Bagby, S., K.I. Tong, and M. Ikura. 2001. Optimization of protein solubility and stability for protein nuclear magnetic resonance. Methods Enzymol. 339:20-41.
4. Bagby, S., K.I. Tong, D. Liu, J.R. Alattia, and M. Ikura. 1997. The button test: a small scale method using microdialysis cells for assessing protein solubility at concentrations suitable for NMR. J. Biomol. NMR 10:279-282.
5. Lepre, C.A. and J.M. Moore. 1998. Microdrop screening: a rapid method to optimize solvent conditions for NMR spectroscopy of proteins. J. Biomol. NMR 12:493-499.
6. Collins, B.K., S.J. Tomanicek, N. Lyamicheva, M.W. Kaiser, and T.C. Mueser. 2004. A preliminary solubility screen used to improve crystallization trials: crystallization and preliminary X-ray structure determination of Aeropyrum pernix flap endonuclease-1. Acta Crystallogr. D Biol. Crystallogr. 60:1674-1678.
7. Alexandrov, A., K. Dutta, and S.M. Pascal. 2001. MBP fusion protein with a viral protease cleavage site: one-step cleavage/purification of insoluble proteins. BioTechniques 30:1194-1198.
8. Cordingley, M.G., P.L. Callahan, V.V. Sardana, V.M. Garsky, and R.J. Colonno. 1990. Substrate requirements of human rhinovirus 3C protease for peptide cleavage in vitro. J. Biol. Chem. 265:9062-9065.
9. Quiocho, F.A., J.C. Spurlino, and L.E. Rodseth. 1997. Extensive features of tight oligosaccharide binding revealed in high-resolution structures of the maltodextrin transport/chemosensory receptor. Structure 5:997-1015.
10. Golovanov, A.P., G.M. Hautbergue, S.A. Wilson, and L.Y. Lian. 2004. A simple method for improving protein solubility and long-term stability. J. Am. Chem. Soc. 126:8933-8939.
11. Davis-Searles, P.R., A.J. Saunders, D.A. Erie, D.J. Winzor, and G.J. Pielak. 2001. Interpreting the effects of small uncharged solutes on protein-folding equilibria. Annu. Rev. Biophys. Biomol. Struct. 30:271-306.
12. Jancarik, J., R. Pufan, C. Hong, S.H. Kim, and R. Kim. 2004. Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins. Acta Crystallogr. D Biol. Crystallogr. 60:1670-1673.
13. Luft, J.R., R.J. Collins, N.A. Fehrman, A.M. Lauricella, C.K. Veatch, and G.T. DeTitta. 2003. A deliberate approach to screening for initial crystallization conditions of biological macromolecules. J. Struct. Biol. 142:170-179.