Microdrop screening: A rapid method to optimize solvent conditions for NMR spectroscopy of proteins

Journal of Biomolecular NMR

Volumn 12, Issue 4, 1998, Pages 493-499

  Lepre, Christopher A ^a   Moore, Jonathan M ^a  

^a VERTEX PHARMACEUTICALS   (United States)

Author keywords

Hanging drop; NMR sample preparation; Protein solubility; Vapor diffusion

Indexed keywords

BUFFER; GLIA MATURATION FACTOR; NERVE GROWTH FACTOR; NERVE PROTEIN; PROTEIN; RECOMBINANT PROTEIN; SOLVENT;

ARTICLE; CHEMISTRY; COMPARATIVE STUDY; HUMAN; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE; PROTEIN CONFORMATION;

BUFFERS; GLIA MATURATION FACTOR; HUMANS; NERVE GROWTH FACTORS; NERVE TISSUE PROTEINS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN CONFORMATION; PROTEINS; RECOMBINANT PROTEINS; SOLVENTS;

EID: 0032201444     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008353000679     Document Type: Article

References (9)

1. Bagby, S., Tong, K., Liu, D., Alattia, J.-R. and Ikura, M. (1997) J. Biomol. NMR, 10, 279-282.
2. Ducruix, A. and Giegé, R. (1992) Crystallization of Nucleic Acids and Proteins: A Practical Approach, Oxford University Press, New York, NY.
3. Jancarik, J. and Kim, S. (1991) J. Applied Crystallogr., 24, 409-411.
4. Kaplan, R., Zaheer, A., Jaye, M. and Lim, R. (1991) J. Neurochem., 57, 483-490.
5. Matthews, S.J. and Leatherbarrow, R.J. (1993) J. Biomol. NMR, 3, 597-600.
6. McPherson, A. (1990) Eur. J. Biochem., 189, 1-23.
7. Santoro, M.M., Liu, Y., Khan, S., Hou, L-X., and Bolen, D.W. (1992) Biochemistry, 31, 5278-5283.
8. Wagner, G. (1993) J. Biomol. NMR, 3, 375-385.
9. Yancey, P.H., Clark, M.E., Hand, S.C., Bowlus, R.D. and Somero, G.N. (1982) Science, 217, 1214-1222.