Maize uroporphyrinogen III methyltransferase: Overexpression of the functional gene fragments in Escherichia coli and one-step purification

Protein Expression and Purification

Volumn 46, Issue 1, 2006, Pages 40-46

  Fan, Jun ^a,b,c   Wang, Deqiang ^a,b   Liang, Zhi ^a,b   Guo, Min ^a,b   Teng, Maikun ^a,b   Niu, Liwen ^a,b  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^b UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^c ANHUI AGRICULTURAL UNIVERSITY   (China)

Author keywords

Maize; Purification; Red fluorescent compounds; Uroporphyrinogen III methyltransferase

Indexed keywords

ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; METHYLTRANSFERASE; UROPORPHYRIN III C METHYLTRANSFERASE; UROPORPHYRIN-III C-METHYLTRANSFERASE; VEGETABLE PROTEIN;

ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; ENZYMOLOGY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENE DELETION; GENETICS; ISOLATION AND PURIFICATION; MAIZE; METABOLISM; MOLECULAR CLONING; MOLECULAR WEIGHT; MUTAGENESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; RESTRICTION MAPPING;

CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; METHYLTRANSFERASES; MOLECULAR WEIGHT; MUTAGENESIS; PLANT PROTEINS; RECOMBINANT FUSION PROTEINS; RESTRICTION MAPPING; SEQUENCE DELETION; ZEA MAYS;

ESCHERICHIA COLI; ZEA MAYS;

EID: 32644476276     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.07.010     Document Type: Article

References (18)

1. 12) Nat. Prod. Rep. 19 2002 390 412
2. C. Robin, F. Blanche, L. Cauchois, B. Cameron, M. Couder, and J. Crouzet Primary structure, expression in Escherichia coli, and properties of S-adenosyl-l-methionine: uroporphyrinogen III methyltransferase from Bacillus megaterium J. Bacteriol. 173 1991 4893 4896
3. F. Blanche, L. Debussche, D. Thibaut, J. Crouzet, and B. Cameron Purification and characterization of S-adenosyl-l-methionine: uroporphyrinogen III methyltransferase from Pseudomonas denitrificans J. Bacteriol. 171 1989 4222 4231
4. 12 Gene 27 2001 63 70
5. M.J. Warren, E.L. Bolt, C.A. Roessner, A.I. Scott, J.B. Spencer, and S.C. Woodcock Gene dissection demonstrates that the Escherichia coli cysG gene encodes a multifunctional protein Biochem. J. 302 1994 837 844
6. J. Ve'vodova, R.M. Graham, E. Raux, H.L. Schubert, D.I. Roper, A.A. Brindley, A.I. Scott, C.A. Roessner, N.P.J. Stamford, M.E. Stroupe, E.D. Getzoff, M.J. Warren, and K.S. Wilson Structure/function studies on a S-adenosyl-l-methionine-dependent uroporphyrinogen III C methyltransferase (SUMT), a key regulatory enzyme of tetrapyrrole biosynthesis J. Mol. Biol. 344 2004 419 433
7. M.E. Stroupe, H.K. Leech, D. Daniels, M.J. Warren, and E.D. Getzoff CysG structure reveals tetrapyrrole-binding features and novel regulation of siroheme biosynthesis Nat. Struct. Biol. 10 2003 1064 1073
8. M.J. Warren, N.J. Stolowich, P.J. Santander, C.A. Roessner, B.A. Sowa, and A.I. Scott Enzymatic synthesis of dihydrosirohydrochlorin (precorrin-2) and of a novel pyrrocorphin by uroporphyrinogen III methylase FEBS Lett. 261 1990 76 80
9. I. Sattler, C.A. Roessner, N.J. Stolowich, S.H. Hardin, L.W. Harris-Haller, N.T. Yokubaitis, Y. Murooka, Y. Hashimoto, and A.I. Scott Cloning, sequencing, and expression of the uroporphyrinogen III methyltransferase cobA gene of Propionibacterium freudenreichii (shermanii) J. Bacteriol. 177 1995 1564 1569
10. C.A. Roessner, and A.I. Scott Fluorescence-based method for selection of recombinant plasmids Biotechniques 19 1995 760 764
11. S. Wildt, and U. Deuschle CobA, a red fluorescent transcriptional reporter for Escherichia coli, yeast, and mammalian cells Nat. Biotechnol. 17 1999 1175 1178
12. T. Leustek, M. Smith, M. Murillo, P.S. Singh, A.G. Smith, S.C. Woodcock, S.J. Awan, and M.J. Warren Siroheme biosynthesis in higher plants. Analysis of an S-adenosyl-l-methionine- dependent uroporphyrinogen III methyltransferase from Arabidopsis thaliana J. Biol. Chem. 272 1997 2744 2752
13. H. Sakakibara, K. Takei, and T. Sugiyama Isolation and characterization of a cDNA that encodes maize uroporphyrinogen III methyltransferase, an enzyme involved in the synthesis of siroheme, which is a prosthetic group of nitrite reductase Plant J. 10 1996 883 892
14. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
15. P.K. Smith, R.I. Krohn, G.T. Hermanson, A.K. Mallia, F.H. Gartner, M.D. Provenzano, E.K. Fujimoto, N.M. Goeke, B.J. Olson, and D.C. Klenk Measurement of protein using bicinchoninic acid Anal. Biochem. 150 1985 76 85
16. E. Raux, T. Mcveigh, S.E. Peters, T. Leustek, and M.J. Warren The role of Saccharomyces cerevisiae Met1p and Met8p in siroheme and cobalamin biosynthesis Biochem. J. 338 1999 701 708
17. P.L. Chou, M. Ohtsuka, T. Minowa, K. Yamasato, Y. Sakano, H. Matsuzawa, T. Ohta, and H. Sakai Reddish Escherichia coli cells caused by overproduction of Bacillus stearothermophilus uroporphyrinogen III methylase: cloning, sequencing and expression of the gene Biosci. Biotech. Biochem. 59 1995 1817 1824
18. F. Blanche, C. Robin, M. Couder, L. Cauchois, B. Cameron, and J. Crouzet Purification, characterization, and molecular cloning of S-adenosyl-l- methionine: uroporphyrinogen III methyltransferase from Methanobacterium ivanovii J. Bacteriol. 173 1991 4637 4645