Effective isolation of MPB64 from a large volume of culture filtrate of Mycobacterium bovis BCG Tokyo

Japanese Journal of Medical Science and Biology

Volumn 49, Issue 1, 1996, Pages 15-27

  Haga, Shinji ^a   Kawajiri, Katsuhide ^b   Niinuma, Sayoko ^a,b   Honda, Ikuro ^b   Yamamoto, Setsuko ^c   Toida, Ichiro ^b   Nakamura, Reiko M ^b   Nagai, Sadamu ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^b Kiyose   (Japan)

^c RESEARCH INSTITUTE OF TUBERCULOSIS   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

2 DIETHYLAMINOETHANOL; SECRETORY PROTEIN; SEPHAROSE; UREA;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIUM CULTURE; COMPARATIVE STUDY; CULTURE MEDIUM; GUINEA PIG; IMMUNOBLOTTING; MOLECULAR WEIGHT; MYCOBACTERIUM BOVIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ISOLATION; SKIN MANIFESTATION;

BACTERIA (MICROORGANISMS); CAVIA; CAVIA PORCELLUS; MYCOBACTERIUM; MYCOBACTERIUM BOVIS BCG; SUS SCROFA;

EID: 0029931226     PISSN: 00215112     EISSN: None     Source Type: Journal    
DOI: 10.7883/yoken1952.49.15     Document Type: Article

References (14)

1. Harboe, M., Nagai, S., Patarroyo, M. E., Torres, M. L., Ramirez, C. and Cruz, N. (1986): Properties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis BCG. Infect. Immun., 52, 293-302.
2. Nagai, S., Wiker, H. G., Harboe, M. and Kinomoto, M. (1991): Isolation and partial characterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis. Infect. Immun., 59, 372-382.
3. Yamaguchi, R., Matsuo, K., Yamazaki, A., Abe, C., Nagai, S., Terasaka, K. and Yamada, T. (1989): Cloning and characterization of the gene for immunogenic protein MPB64 of Mycobacterium bovis BCG. Infect. Immun., 57, 283-288.
4. Oettinger, T. and Andersen, Å. B. (1994): Cloning and B-cell-epitope mapping of MPT64 from Mycobacterium tuberculosis H37Rv. Infect. Immun., 62, 2058-2064.
5. Li, H., Ulstrup, J. C., Jonassen, T. Ø., Melby, K., Nagai, S. and Harboe, M. (1993): Evidence for absence of the MPB64 gene in some substrains of Mycobacterium bovis BCG. Infect. Immun., 61, 1730-1734.
6. Hasløv, K., Andersen, Å., Nagai, S., Gottschau, A., Sørensen, T. and Andersen, P. (1995): Guinea pig cellular immune responses to proteins secreted by Mycobacterium tuberculosis. Infect. Immun., 63, 804-810.
7. Haga, S., Yamaguchi, R., Nagai, S., Matsuo, K., Yamazaki, A. and Nakamura, R. M. (1995): Delayed-type hypersensitivity to a recombinant mycobacterial antigen, MPB64, in guinea pigs sensitized to Mycobacterium tuberculosis or Mycobacterium bovis BCG. J. Leuk. Biol., 57, 221-225.
8. Nagai, S., Nagasuga, T., Matsumoto, J. and Kohda, K. (1974): Isolation of tuberculin skin test reactive proteins from heated culture filtrate of Mycobacterium tuberculosis H37Rv. Am. Rev, Resp. Dis., 109, 17-28.
9. O'Farrell, P. H. (1975): High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem., 250, 4007-4021.
10. Wiker, H. G., Harboe, M. and Nagai, S. (1991): A localization index for distinction between extracellular and intracellular antigens of Mycobacterium tuberculosis. J. Gen. Microbiol., 137, 875-884.
11. Bradford, M. M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254.
12. Nagai, S., Matsumoto, J. and Nagasuga, T. (1981): Specific skin-reactive protein from culture filtrate of Mycobacterium bovis BCG. Infect. Immun., 31, 1152-1160.
13. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951): Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-275.
14. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J. and Klenk, D. C. (1985): Measurement of protein using bicinchoninic acid. Anal. Biochem., 150, 76-85.