A fluorescence-based assay for the reductase activity of protein disulfide isomerase

Analytical Biochemistry

Volumn 350, Issue 1, 2006, Pages 105-112

  Tomazzolli, Rossella ^a   Serra, Mauro Dalla ^a   Bellisola, Giuseppe ^b   Colombatti, Marco ^b   Guella, Graziano ^c  

^a CNR   (Italy)

^b UNIVERSITY OF VERONA   (Italy)

^c UNIVERSITY OF TRENTO   (Italy)

Author keywords

FITC derivatives; Fluorescence self quenching; NMR characterization; Protein disulfide isomerase; Thioredoxin reductase

Indexed keywords

COVALENT BONDS; DIMETHYL SULFOXIDE; FLUORESCENCE; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE;

FLUORESCEIN ISOTHIOCYANATE; FLUORESCENCE ENHANCEMENT; FLUORESCENCE SELF-QUENCHING; FUNCTIONAL GROUP TRANSFORMATIONS; PROTEIN DISULFIDE ISOMERASES; QUENCHED SUBSTRATES; SPECTROFLUORIMETRIC; THIOREDOXIN REDUCTASE;

PROTEINS;

ASPARTIC ACID; CYSTEINE; FLUORESCEIN ISOTHIOCYANATE; GLYCINE; OXIDOREDUCTASE; PROTEIN DISULFIDE ISOMERASE; THIOREDOXIN; THIOREDOXIN REDUCTASE;

ARTICLE; DISULFIDE BOND; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE ANALYSIS; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; REDUCTION;

EID: 32644454393     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.11.037     Document Type: Article

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