Improvement of reverse transcription PCR by RNase H

Bioscience, Biotechnology and Biochemistry

Volumn 67, Issue 11, 2003, Pages 2474-2476

  Kitabayashi, Masao ^a   Esaka, Muneharu ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Family B DNA polymerase; High fidelity; KOD Plus DNA polymerase; Reverse Transcription PCR (RT PCR); RNase H

Indexed keywords

PRIMER DNA; RIBONUCLEASE H; RNA DIRECTED DNA POLYMERASE;

ARTICLE; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BASE SEQUENCE; DNA PRIMERS; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIBONUCLEASE H, CALF THYMUS; RNA-DIRECTED DNA POLYMERASE; TRANSCRIPTION, GENETIC;

EID: 3242890130     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.67.2474     Document Type: Article

References (5)

1. Chenchik, A., Diachenko, L., Moqadam, F., Tarabykin, V., Lukyanov, S., and Siebert, P. D., Full-length cDNA cloning and determination of mRNA 5′ and 3′ ends by amplification of adaptor-ligated cDNA. BioTechniques, 21, 526-534 (1996).
2. Takagi, M., Nishioka, M., Kakihara, H., Kitabayashi, M., Inoue, H., Kawakami, B., Oka, M., and Imanaka, T., Characterization of DNA polymerase from Pyrococcus sp, strain KOD1 and its application to PCR. Appl. Environ. Microbiol., 63, 4504-4510 (1997).
3. Kitabayashi, M., Nishiya, Y., Esaka, M., Itakura, M., and Imanaka, T., Gene cloning and polymerase chain reaction with proliferating cell nuclear antigen from Thermococcus kodakaraensis KOD1. Biosci. Biotechnol. Biochem., 66, 2194-2200 (2002).
4. Horiuchi, T., Physiological functions of RNase H in Escherichia coli. Tanpakushitsu Kakusan Koso (in Japanese), 31, 132-143 (1986).
5. Kitabayashi, M., Nishiya, Y., and Esaka, M., A simple and efficient method for high fidelity PCR cloning using antibody-neutralizing technology. Biosci. Biotechnol. Biochem., 67, 2034-2037 (2003).