Immunogold-silver staining for light microscopy using colloidal or clustered gold (nanogold®)

Gold and Silver Staining: Techniques in Molecular Morphology

Volumn , Issue , 2002, Pages 47-69

  Hacker, Gerhard W ^a   Cheung, Annie L M ^b   Tubbs, Raymond R ^c   Grimelius, Lars ^d   Danscher, Gorm ^e   Hauser Kronberger, Cornelia ^f  

^a St Johanns Spital   (Austria)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

^c LERNER RESEARCH INSTITUTE   (United States)

^d UPPSALA UNIVERSITY   (Sweden)

^e AARHUS UNIVERSITY   (Denmark)

^f Salzburger Landeskliniken   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 3242876434     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (110)

1. Arai, R. and I. Nagatsu. 1995. Application of gold toning to immunogold-silver staining, p. 209-216. In M.A. Hayat (Ed.), Immunogold-Silver Staining. Principles, Methods, and Applications. CRC Press, Boca Raton.
2. Arens, H. and J. Eggert. 1929. Das Wachstum des kolloidalen Silbers in Gelatineschichten. Z. Elektrochem. 35:728-733.
3. Bishop, A.E., J.M. Polak, S.R. Bloom, and A.G.E. Pearse. 1978. A new universal technique for the immunocytochemical localisation of peptidergic innervation. J. Endocrinol. 77:25-26.
4. Bobrow, M.N., D. Thomas, D. Harris, K.J. Shaughnessy, and G.J. Litt. 1989. Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J. Immunol. Meth. 124:279-285.
5. Bobrow, M.N., K.J. Shaugnessy, and G.J. Litt. 1991. Catalyzed reporter deposition, a novel method of signal amplification. II. Application to membrane immunoassays. J. Immunol. Meth. 137:103-112.
6. Bondi, A., G. Chieregatti, V. Eusebi, E. Fulcheri, and G. Bussolati. 1982. The use of β-galactosidase as a tracer in immunohistochemistry. Histochemistry 76:153-158.
7. Bonnard, C., D.S. Papermaster, and J.P. Kraehenbuhl. 1984. The streptavidin-biotin bridge technique:application in light and electron microscope immunocytochemistry, p. 95-111. In J.M. Polak and I.M. Varndell (Eds.), Immunolabelling for Electron Microscopy. Elsevier, Amsterdam.
8. Burry, R.W., D.D. Vandre, and D.M. Hayes. 1992. Silver enhancement of gold antibody probes in preembedding electron microscopic immunocytochemistry. J. Histochem. Cytochem. 40:1849-1856.
9. Cailliez, J.C., A. Boudrissa, D.W. Mackenzie, and D. Poulain. 1990. Evaluation of a gold-silver staining method for detection and identification of Candida species by light microscopy. Eur. J. Clin. Microbiol. Infect. Dis. 9:886-891.
10. Coggi, G., P. Dell’Orto, and G. Viale. 1986. Avidinbiotin methods, p. 54-70. In J.M. Polak and S. Van Norden (Eds.), Immunocytochemistry-Modern Methods and Applications. Wright, Bristol.
11. Cordell, J.L., B. Falini, W.N. Erber, A.K. Ghosh, Z. Abdulaziz, S. MacDonald, K.A.F. Pulford, H. Stein, and D.Y. Mason. 1984. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J. Histochem. Cytochem. 32:219-229.
12. Cremers, A.F.M., N. Jansen in de Wal, J. Wiegant, R.W. Dirks, P. Weisbeek, M. van der Ploeg, and J.E. Landegent. 1987. Non-radioactive in situ hybridisation. A comparison of several immunocytochemical detection systems using reflection-contrast and electron microscopy. Histochemistry 86:609-615.
13. Danscher, G. 1981. Histochemical demonstration of heavy metals. A revised version of the sulphide silver method suitable for both light and electron microscopy. Histochemistry 71:1-16.
14. Danscher, G. 1981. Localization of gold biological tissue. A photochemical method for light and electron microscopy. Histochemistry 71:81-88.
15. Danscher, G. and J.O. Rytter Nörgaard. 1983. Light microscopic visualisation of colloidal gold on resinembedded. J. Histochem. Cytochem. 31:1394-1398.
16. Danscher, G. 1984. Autometallography. A new technique for light and electron microscopical visualization of metals in biological tissue (gold, silver, metal sulphides, and metal selenides). Histochemistry 81:331-335.
17. Danscher, G., G.W. Hacker, J.O. Norgaard, and L. Grimelius. 1993. Autometallographic silver amplification of colloid gold. J. Histotechnol. 16:201-207.
18. Danscher, G., G.W. Hacker, C. Hauser-Kronberger, and L. Grimelius. 1995. Trends in autometallographic silver amplification of colloidal gold particles, p. 11-18. In M.A. Hayat (Ed.), Immunogold-Silver Staining:Methods and Applications. CRC-Press, Boca Raton.
19. De Bault, L.E. and B.-L. Wang. 1994. Localization of mRNA by in situ transcription and immunogold-silver staining. Cell Vision 1:67-70.
20. De Mey, J., G.W. Hacker, M. De Waele, and D. Springall. 1986. Gold probes in light microscopy, p. 71-88. In J.M. Polak and S. Van Norden (Eds.), Immunocytochemistry-Modern Methods and Applications. Wright, Bristol.
21. De Waele, M., J. De Mey, W. Renmans, C. Labeur, K. Jochmans, and B. Van Camp. 1986b. Potential of immunogold-silver staining for the study of leukocyte subpopulations as defined by monoclonal antibodies. J. Histochem. Cytochem. 34:1257-1263.
22. Ellis, I.O, J. Bell, and J.D. Bancroft. 1988. An investigation of optimal particle size for immunohistological immunogold and immunogold-silver staining to be viewed by polarized incident light (EPI polarization) microscopy. J. Histochem. Cytochem. 36:121-124.
23. Faulk, W.P. and G.M. Taylor. 1971. An immunocolloid method for the electron microscope. Immunochemisty 8:1081-1083.
24. Fritz, P., J. Hoenes, J. Schenk, A. Mischlinsky, A. Grau, J.G. Saal, H.V. Tuczek, H. Multhaupt, and G. Pfleiderer. 1986. Color development of immunogoldlabelled antibodies for light microscopy. Histochemistry 85:209-452.
25. Fujimori, O. and M. Nakamura. 1985. Protein A gold-silver staining method for light microscopic immunohistochemistry. Arch. Histol. Jpn. 48:449-452.
26. Fujimori, O. 1992. A double protein A-gold-silver staining method for tissue antigens in light microscopy. Histochemical J. 24:61-66.
27. Fujimori, O., T. Ueda, and K. Yamada. 1994. Effects of iodine pretreatment of sections upon immunogold- siver staining in light microscopic immunohistochemistry. Okajimas Folia Anat. Jpn. 71:319-323.
28. Geoghegan, W.D., J.J. Scillian, and G.A. Ackerman. 1978. The detection of human B-lymphocytes by both light and electron microscopy utilizing colloidal gold-labelled anti-immunogobulin. Immunol. Commun. 7:1-12.
29. Geuze, H.J., J.W. Slot, K. Yanagibashi, J.-A. McCracken, L.A. Schwartz, and P.-F. Hall. 1987. Immunogold cytochemistry of cytochromes P-450 in porcine adrenal cortex. Histochemistry 87:551-557.
30. Giffin, B.F., K. Gao, R.E. Morris, and R.R. Cardell. 1993. Enhancement of antigenic site detection with gold labeled secondary and tertiary antibodies using the immunogold-silver staining method. Biotech. Histochem. 68:309-315.
31. Gillitzer, R., R. Berger, and H. Moll. 1990. A reliable method for simultaneous demonstration of two antigens using a novel combination of immunogold-silver staining and immunoenzymatic labeling. J. Histochem. Cytochem. 38:307-313.
32. Graf, A.-H., A.L.M. Cheung, C. Hauser-Kronberger, N. Dandachi, R.R. Tubbs, O. Dietze, and G.W. Hacker. 2000. Clinical relevance of HPV 16/18 testing methods in cervical squamous cell carcinoma. Appl. Immunohist. Mol. Morphol. (AIMM) 8:300-309.
33. Gu, J., J. De Mey, M. Moeremans, and J.M. Polak. 1981. Sequential use of the PAP and immunogoldstaining methods for the light microscopical double staining of tissue antigens. Its application to the study of regulatory peptides in the gut. Regul. Pept. 1:365-374.
34. Gu, J., M. Forte, N. Carson, G.W. Hacker, M. D’Andrea, and R. Rufner. 1995. Quantitative evaluation of immunogold-silver staining, p. 119-136. In M.A. Hayat (Ed.), Immunogold-Silver Staining:Methods and Applications. CRC Press, Boca Raton.
35. Guesdon, J.L., T. Ternynck, and S. Avrameas. 1979. The use of avidin-biotin interaction in immunoenzymatic techniques. J. Histochem. Cytochem. 27:1131-1139.
36. Haase, A.T., D. Walker, L. Strowring, P. Ventura, A. Geballe, H. Blum, M. Brahic, R. Goldberg, and K. O’Brien. 1985. Detection of two viral genomes in single cells by double-label hybridisation in situ and colour autoradiography. Science 227:189-192.
37. Hacker, G.W., D.R. Springall, S. Van Noorden, A.E. Bishop, L. Grimelius, and J.M. Polak. 1985. The immunogold-silver staining method. A powerful tool in histopathology. Virchows Arch. A 406:449-461.
38. Hacker, G.W., J.M. Polak, D.R. Springall, J. Ballesta, A. Cadieux, J. Gu, J.Q. Trojanowski, D.Dahl, and P. Marangos. 1985. Antibodies to neurofilament proteins and other brain proteins reveal the innervation of peripheral organs. Histochemistry 82:581-593.
39. Hacker, G.W., D.R. Springall, A. Cheung, S. Van Noorden, and J.M. Polak. 1986. Immunogold-silver staining and its potential use in multiple staining and histopathology [abstract]. J. Histochem. Cytochem. 34:118.
40. Hacker, G.W., L. Grimelius, G. Danscher, G. Bernatzky, W. Muss, H. Adam, and J. Thurner. 1988. Silver acetate autometallography: an alternative enhancement technique for immunogold-silver staining (IGSS) and silver amplification of gold, silver, mercury, and zinc tissues. J. Histotechnol. 11:213.
41. Hacker, G.W. 1989. Silver enhanced colloidal gold for light microscopy, p. 279-321. In M.A. Hayat (Ed.), Colloidal Gold: Principles, Methods, and Applications, Vol. 1., Academic Press, Orlando.
42. Hacker, G.W., A.-H. Graf, C. Hauser-Kronberger, G. Wirnsberger, A. Schiechl, G. Bernatzky, U. Wittauer, H. Su, H. Adam, J. Thurner et al. 1993. Application of silver acetate autometallography and gold-silver staining methods for in situ DNA hybridisation. Chin. Med. J. 106:83-92.
43. Hacker, G.W. and G. Danscher. 1994. Recent advances in immunogold-silver staining-autometallography. Cell Vision 1:102-109.
44. Hacker, G.W., G. Danscher, L. Grimelius, C. Hauser-Kronberger, W.H. Muss, J. Gu, and O. Dietze. 1995. Silver staining techniques, with special reference to the use of different silver salts in lightand electron microscopical immunogold-silver staining, p. 20-45. In M.A. Hayat (Ed.), Immunogold-Silver Staining: Methods and Applications. CRC Press, Boca Raton.
45. Hacker, G.W., I. Zehbe, J. Hainfeld, J. Sällström, C. Hauser-Kronberger, A.-H. Graf, H. Su, O. Dietze, and O. Bagasra. 1996. High-performance Nanogold™ in situ hybridisation and in situ PCR. Cell Vision 3:209-214.
46. Hacker, G.W., W.H. Muss, C. Hauser-Kronberger, G. Danscher, R. Rufner, J. Gu, H. Su, A. Andreasen, M. Stoltenberg, and O. Dietze. 1996. Electron microscopical autometallography: immunogold-silver staining (IGSS) and heavy-metal histochemistry. Methods (Companion Methods Enzymol.) 10:257-269.
47. Hacker, G.W. and G. Danscher. 1997. Immunogold- silver staining-autometallography: recent developments and protocols, p. 41-54. In J. Gu (Ed.), Analytical Morphology-Theory, Protocols, and Applications. Eaton Publishing, Natick, MA.
48. Hacker, G.W., C. Hauser-Kronberger, I. Zehbe, H. Su, A. Schiechl, O. Dietze, and R. Tubbs. 1997. In situ localization of DNA and RNA sequences: supersensitive in situ hybridisation using streptavidin-Nanogold-silver staining: minireview, protocols, and possible applications. Cell Vision 4:54-67.
49. Hacker, G.W. 1998. High-performance Nanogold-silver in situ hybridisation. Eur. J. Histochem. 42:111-120.
50. Hainfeld, J.F. 1987. A small gold-conjugated antibody label: improved resolution for electron microscopy. Science 236:450-453.
51. Hainfeld, J.F. and F.R. Furuja. 1992. A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling. J. Histochem. Cytochem. 30:177-184.
52. Hauser-Kronberger, C., G.W. Hacker, E. Arrer, and G. Danscher. 1995. Nonmicroscopical colloidal gold autometallography (AMGAu): use of immunogold-silver staining in blot staining and immuonassay, p. 289-297. In M.A. Hayat (Ed.), Immunogold-Silver Staining. Principles, Methods, and Applications. CRC Press, Boca Raton.
53. Holgate, C.S., P. Jackson, P.N. Cowen, and C.C. Bird. 1983. Immunogold-silver staining: new method of immunostaining with enhanced sensitivity. J. Histochem. Cytochem. 31:938-994.
54. Holgate, C.S., P. Jackson, I. Lauder, P. Cowen, and C. Bird. 1983. Surface membrane staining of immunoglobulins in paraffin sections of non-Hodgkin’s lymphomas using immunogold-silver staining technique. J. Clin. Pathol. 36:742-746.
55. Horisberger, M. 1981. Colloidal gold: a cytochemical marker for light and fluorescent microscopy and for transmission and scanning electron microscopy. Scanning Electron Microsc. II:9-31.
56. Horisberger, M. and M.F. Clerc. 1985. Labelling of colloidal gold with protein A. A quantitative study. Histochemistry 82:219-223.
57. Jackson, P. 1985. Staining with silver and gold. Med. Lab. World 10:16-26.
58. King, T.P., L. Brydon, G.W. Gooday, and L.H. Chappell. 1987. Silver enhancement of lectin-gold and enzym-gold cytochemical labelling of eggs of the nematode Onchocerca gibsoni. Histochem. J. 19:281-286.
59. Krenács, T., L. Krenács, B. Bozoky, and B. Ivanyi. 1990. Double and triple immunocytochemical labelling at the light microscope level in histopathology. Histochem. J. 22:530-536.
60. Krenács, T. and L. Krenács. 1997. Immunogold-silver staining (IGSS) for single and multiple antigen detection in archived tissues following antigen retrieval. Cell Vision 4:387-393.
61. Lackie, P.M., R.J. Hennessy, G.W. Hacker, and J.M. Polak. 1985. Investigation of immunogold-silver staining by electron microscopy. Histochemistry 83:545-550.
62. Leathem, A. 1986. Lectin histochemistry, p. 167-187. In J.M. Polak and S. Van Noorden (Eds.), Immunocytochemistry-Modern Methods and Applications. Wright, Bristol.
63. Liesi, P., J.-P. Julien, P. Vilja, F. Grosveld, and L. Rechardt. 1986. Specific detection of neuronal cell bodies: in situ hybridisation with a biotin-labeled neurofilament cDNA probe. J. Histochem. Cytochem. 34:923-926.
64. Liu, Y.F. 1987. An experimental investigation of the colored-IGSS method. Chung Hua Ping Li Hsueh Tsa Chih. 16:248-250.
65. Liu, Y.S., W.P. Du, and Z.X. Wu. 1996. Dotimmunogold- silver staining in the diagnosis of cysticercosis. Int. J. Parasitol. 26:127-129.
66. Löning, T., R.-P. Henke, P. Reichart, and J. Becker. 1987. In situ hybridisation to detect Epstein-Barr virus DNA in oral tissues of HIV-infected patients. Virchows Arch. A. 412:127.
67. Marchetti, A., M. Bistocchi, and A.R. Tognetti. 1987. Silver enhancement of protein A-gold probes on resin-embedded ultrathin sections. Histochemistry 86:371-373.
68. Menetrey, D. 1985. Retrograde tracing of neural pathways with a protein-gold complex. I. Light microscopic detection after silver intensification. Histochemistry 83:391-395.
69. Moeremans, M., G. Daneels, A. Van Dijk, G. Langanger, and J. De Mey. 1984. Sensitive visualisation of antigen antibody reaction in dot and blot immuneoverlay assays with immunogold and immunogold-silver staining. J. Immunol. Methods 74:353-360.
70. Nakane, P.K. and G.B. Pierce. 1966. Enzyme-labelled antibodies: preparation and application for the localization of antigens. J. Histochem. Cytochem. 14:929-931.
71. Nyhlin, N., M. el-Salhy, O. Sandstrom, and O. Suhr. 1997. Evaluation of immunohistochemical staining of human duodenal endocrine cells after microwave antigen retrieval. Histochem. J. 29:177-181.
72. Petrusz, P., P. DiMeo, P. Ordronneau, C. Weaver, and D.A. Keefer. 1975. Improved immunoglobulinenzyme bridge method for light microscopic demonstration of hormone-containing cells of the rat adenohypophysis. Histochemistry 46:9-15.
73. Petrusz, P., P. Ordronneau, and J.C.W. Finley. 1980. Criteria of reliability for light microscopic immunocytochemical staining. Histochem. J. 12:333-348.
74. Powell, R.D., C.M.R. Halsey, D.L. Spector, S.L. Kaurin, J. McCann, and J.F. Hainfeld. 1997. A covalent fluorescent-gold immunoprobe: simultaneous detection of a pre-mRNA splicing factor by light and electron microscopy. J. Histochem. Cytochem. 45:947-956.
75. Robinson, J.M. and T. Takizawa. 1996. Novel labeling methods for EM analysis of ultrathin cryosections, p. 894-895. In G.W. Bailey, J.M. Corbett, R.V.W. Dimlich, J.R. Michael, and N.J. Zaluzec (Eds.), Proc. Micros. Microanal. San Francisco Press, San Francisco.
76. Robinson, J.M. and D.D. Vandré. 1997. Efficient immunocytochemical labeling of leukocyte microtubules with FluoroNanogold: an important tool for correlative microscopy. J. Histochem. Cytochem. 45:631-642.
77. Roth, J. 1982. Applications of immunocolloids in light microscopy. Preparation of protein A-silver and protein A-gold complexes and their application for the localisation of single and multiple antigens in paraffin sections. J. Histochem. Cytochem. 30:691-696.
78. Roth, J. 1983. The colloid gold marker system for light and electron microscopic cytochemistry. Immunocytochemistry 2:217-284.
79. Rufner, R., N.E. Carson, M. Forte, G. Danscher, J.Gu, and G.W. Hacker. 1995. Autometallography for immunogold-silver staining. in light and electron microscopy. Cell Vision 2:327-333.
80. Sako, H., Y. Nakane, K. Okino, K. Nishihara, M. Kodama, M. Kawata, and H. Yamada. 1986. Simultaneous detection of B-cells and T-cells by a double immunohistochemical technique using immunogold- silver staining and the avidin-biotin-peroxidase complex. Histochemistry 86:1-4.
81. Schaumburg-Lever, G. and M. Tronnier. 1989. Combination of the APAAP and the IGSS techniques for double-labeling with two different monoclonal antibodies. Am. J. Dermatopathol. 11:29-32.
82. Schmidt, J. and W. Peters. 1987. Localization of glycoconjugates at the tegument of the tapeworms Ymenolepis nana and H. microstoma with gold labelled lectins. Parasitol. Res. 73:80.
83. Scopsi, L. and L.I. Larsson. 1985. Increased sensitivity in immunocytochemistry. Effects of double application of antibodies and silver intensification on immunogold and peroxidase-antiperoxidase staining techniques. Histochemistry 82:321-329.
84. Scopsi, L., L.I. Larsson, L. Bastholm, and M. Hartvig Nielsen. 1986. Silver-enhanced-colloidal gold probes as markers for scanning electron microscopy. Histochemistry 86:35-41.
85. Shi, S.-R., M.E. Key, and K.L. Kalra. 1991. Antigen retrieval in formalin fixed, paraffin-embedded tissues:an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 39:741-748.
86. Shi, S.-R., J. Gu, K.L. Kalra, T. Chen, R.J. Cote, and C.R. Taylor. 1995. Antigen retrieval technique: a novel approach to immunohistochemistry on routinely processed tissue sections. Cell Vision 2:6-22.
87. Skutelsky, E., V. Goyal, and J. Alroy. 1987. The use of avidin-gold complex for light microscopic localization of lectin receptors. Histochemistry 86:291-295.
88. Springall, D.R., G.W. Hacker, L. Grimelius, and J.M. Polak. 1984. The potential of the immunogold- silver staining method for paraffin sections. Histochemistry 81:603-608.
89. Springall, D.R. 1986. Immunocytochemistry in diagnostic cytology, p.547-567. In J.M. Polak and S. Van Noorden (Eds.), Immunocytochemistry-Modern Methods and Applications. Wright, Bristol.
90. Stefanini, M., C. DeMartino, and L. Zamboni. 1967. Fixation of ejaculated spermatozoa for electron microscopy. Nature 216:173-174.
91. Sternberger, L.A., P.H. Hardy, J.J. Cuculis, and H.G. Meyer. 1970. The unlabeled antibody-enzyme method for immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use for identification of spirochetes. J. Histochem. Cytochem. 18:315-333.
92. Tajima, Y., K. Kato, N. Utsumi, and K. Hosoi. 1994. Computer-aided image analysis applied to immunogold- silver staining: evaluation of proliferating cell nuclear antigen (PCNA)-reactive sites in paraffin sections. Histochemistry 102:177-181.
93. Takizawa, T. and J.M. Robinson. 1994. Use of 1.4- nm immunogold particles for immunocytochemistry on ultra-thin cryosections. J. Histochem. Cytochem. 43:1615-1623.
94. Tbakhi, A., G. Totos, C. Hauser-Kronberger, J. Pettay, D. Baunnoch, G.W. Hacker, and R.R. Tubbs. 1998. Fixation conditions for and RNA in situ hybridization: a reassessment of molecular morphology dogma. Am. J. Pathol. 152:35-41.
95. Teasdale, J., P. Jackson, C.S. Holgate, and P.N. Cowen. 1987. Identification of oestrogen receptors in cells of paraffin-processed breast cancers by IGSS. Histochemistry 87:185-187.
96. Terazawa, K., G.D. Griffiths, A.G. Leith, and M.A. Green. 1989. Electrophoretic determination of ricin using immunogold-silver staining-comparison with simple “protein dot” method. Nippon Hoigaku Zasshi. 43:303-309.
97. Totos, G., A. Tbakhi, C. Hauser-Kronberger, and R.R. Tubbs. 1997. Catalyzed reporter deposition: a new era in molecular immunomorphology. Nanogold-silver staining and colorimetric detection and protocols. Cell Vision 4:433-442.
98. Tronnier, M., G. Schaumburg-Lever, and G. Rassner. 1989. A comparison of immunohistologic technics in dermatopathology. Hautarzt 40:259-265.
99. Ueda, T., S. Kawamura, O. Hattori, and O. Fujimori. 1994. Dual staining methods for carbohydrates using lectin-gold-silver techniques. Okajimas Folia Anat. Jpn. 70:301-311.
100. Van Den Pol, A.N. 1985. Silver-intensified gold and peroxidase as dual immunolabels for preand postsynaptic neuromitters. Science 228:332-335.
101. Van Gijlswijk, R.P., H.J. Zijlmans, J. Wiegant, M.N. Bobrow, T.J. Erickson, K.E. Adler, H.J. Tanke, and A.K. Raap. 1997. Fluorochromelabelled tyramides: use in immunocytochemistry and fluorescence in situ hybridisation. J. Histochem. Cytochem. 45:375-382.
102. Van Laere, O., L. De Wael, and J. De Mey. 1985. Immuno gold staining (IGS) and immuno gold- silver staining (IGSS) for the identification of the plant pathogenetic bacterium Erwinia amylovora (Burill) Winslow et al. Histochemistry 83:397-399.
103. Van Noorden, S. 1986. Tissue preparation and immunostaining techniques for light microscopy, p. 26-53. In J.M. Polak and S. Van Noorden (Eds.), Immuncytochemistry-Modern Methods and Applications. Wright, Bristol.
104. Varndell, I.M., J.M. Polak, K.L. Sikri, C.D. Minth, S.R. Bloom, and J.E. Dixon. 1984. Visualisation of messenger RNA directing peptide synthesis by in situ hybridisation using a novel single-stranded cDNA probe. Histochemistry 81:597-601.
105. Westermark, K., M. Lundqvist, G.W. Hacker, A. Karlsson, and B. Westermark. 1987. Growth factor receptors in thyroid follicle cells. Acta Endocrinol. Suppl. (Copenh) 281:252-255.
106. Zamboni, L. and C. De Martino. 1967. Buffered picric acid-formaldehyde: a new fixative for electron microscopy. J. Cell Biol. 35:148A.
107. Zehbe, I., G.W. Hacker, J. Sällström, E. Rylander, and E. Wilander. 1992. In situ polymerase chain reaction (in situ PCR) combined with immunoperoxidase staining and immunogold-silver staining (IGSS) techniques. Detection of single copies of HPV in SiHa cells. Anticancer Res. 12:2165-2168.
108. Zehbe, I., G.W. Hacker, W.H. Muss, J. Sällström, E. Rylander, A.-H. Graf, H. Prömer, and E. Wilander. 1993. An improved protocol of in situ polymerase chain reaction (PCR) for the detection of human papillomavirus (HPV). J. Cancer Res. Clin. Oncol. 119 (Suppl):22S.
109. Zehbe, I., G.W. Hacker, J.F. Sällström, E. Rylander, and E. Wilander. 1994. Self-sustained sequence replication-based amplification (3SR) for the in situ detection of mRNA in cultured cells. Cell Vision 1:20-24.
110. Zehbe, I., G.W. Hacker, H. Su, C. Hauser-Kronberger, J.F. Hainfeld, and R. Tubbs. 1997. Single human papillomavirus copy detection in formalinfixed samples: sensitive in situ hybridization with catalyzed reporter deposition, streptavidin-Nanogold, and silver acetate autometallography. Am. J. Pathol. 150:1553-1561.