Efficient synthesis of nonnatural mutants in Escherichia coli S30 in vitro protein synthesizing system

Journal of Bioscience and Bioengineering

Volumn 97, Issue 6, 2004, Pages 395-399

  Yamanaka, Katsuhiro ^a   Nakata, Hidetaka ^a   Hohsaka, Takahiro ^b   Sisido, Masahiko ^a  

^a OKAYAMA UNIVERSITY   (Japan)

^b JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Chemical aminoacylation; Cyclic tRNA; Four base codons; in vitro synthesis; Nonnatural mutants

Indexed keywords

AMINO ACIDS; GENES; MUTAGENESIS; PROTEINS; RNA;

AMINOACYLATION; MUTANTS;

ESCHERICHIA COLI;

MESSENGER RNA; MUTANT PROTEIN; STREPTAVIDIN; TRANSFER RNA;

AMINOACYLATION; ANTICODON; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; IN VITRO STUDY; MUTAGENESIS; NONHUMAN; OXIDATION; PROTEIN SYNTHESIS; WILD TYPE;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 3242808325     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(04)70225-X     Document Type: Review

References (15)

1. Spirin, A. S.: Cell-free translation systems. Springer Verlag, Berlin/Heidelberg/New York (2002).
2. Spirin, A. S., Baranov, V. I., Ryabova, L. A., Ovodov, S. Y., and Alakhov, Y. B.: A continuous cell-free translation system capable of producing polypeptides in high yield. Science, 242, 1162-1164 (1988).
3. Kigawa, T., Yabuki, T., Yoshida, Y., Tsutsui, M., Ko, Y., Shibata, T., and Yolioyama, S.: Cell-free production and stable-isotope labeling of milligram quantities of proteins. FEBS Lett., 442, 15-19 (1999).
4. Madin, K., Sawasaki, T., Ogasawara, T., and Endo, Y.: A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes. Proc. Natl. Acad. Sci. USA, 97, 559-564 (2000).
5. Hohsaka, T., Ashizuka, Y., Murakami, H., and Sisido, M.: Incorporation of nonnatural amino acids into streptavidin through in vitro frame-shift suppression. J. Am. Chem. Soc., 118, 9778-9779 (1996).
6. Wang, L., Brock, A., Herberich, B., and Schultz, P. G.: Expanding the genetic code of Escherichia coli. Science, 292, 498-500 (2001).
7. Wang, L., Brock, A., and Schultz, P. G.: Adding 1-3-(2-naphthyl)alanine to the genetic code of E. coli. J. Am. Chem. Soc., 124, 1836-1837 (2002).
8. Chin, J.W., Santoro, S.W., Martin, A.B., King, D.S., Wang, L., and Schultz, P. G.: Addition of p-azido-1-phenylalanine to the genetic code of Escherichia coli. J. Am. Chem. Soc., 124, 9026-9027 (2002).
9. Chin, J. W., Martin, A. B., King, D. S., Wang, L., and Schultz, P. G.: Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli. Proc. Natl. Acad. Sci. USA, 99, 11020-11024 (2002).
10. Heckler, T. G., Chang, L. H., Zama, Y., Naka, T., Chorghade, M. S., and Hecht, S. M.: T4 RNA ligase mediated preparation of novel "chemically misacylated" tRNAPheS. Biochemistry, 23, 1468-1473 (1984).
11. Marakami, H., Hohsaka, T., and Sisido, M.: Site-directed incorporation of p-nitrophenylalanine into streptavidin and site-to-site photoinduced electron transfer from a pyrenyl group to a nitrophenyl group on the protein framework. J. Am. Chem. Soc., 120, 7520-7529 (1998).
12. Hohsaka, T., Kajihara, D., Ashizuka, Y., Murakami, H., and Sisido, M.: Efficient incorporation of nonnatural amino acids with large aromatic groups into streptavidin in in vitro protein synthesizing systems. J. Am. Chem. Soc., 121, 34-40 (1999).
13. Hohsaka, T., Ashizuka, Y., Taira, H., Murakami, H., and Sisido, M.: Incorporation of nonnatural amino acids into proteins by using various four-base codons in an Escherichia coli in vitro translation system. Biochemistry, 40, 11060-11064 (2001).
14. Bruce, A. G. and Uhlenbeck, O. C.: Reactions at the termini of tRNA with T4 RNA ligase. Nucleic Acids Res., 5, 3665-3677 (1978).
15. Met with a TV-replacement loop by ligation of chemically synthesized RNA fragments. Nucleic Acids Res., 24, 662-667 (1996).