Incorporation of nonnatural amino acids into streptavidin through in vitro frame-shift suppression

Journal of the American Chemical Society

Volumn 118, Issue 40, 1996, Pages 9778-9779

  Hohsaka, Takahiro ^a   Ashizuka, Yuki ^a   Murakami, Hiroshi ^a   Sisido, Masahiko ^a  

^a OKAYAMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

STREPTAVIDIN;

ARTICLE; PROTEIN SYNTHESIS; RECOMBINANT DNA TECHNOLOGY;

EID: 0029968958     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9614225     Document Type: Article

References (12)

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8. NCCU(-CA) is pGCG GAU UUA GCU CAG UUG GGA GAG CGC CAG ACU NCCU AAU CUG GAG GUC CUG UGU UCG AUC CAC AGA AUU CGC AC.
9. (a) Heckler, T. G.; Chang, L. -H.; Zama, Y.; Naka, T.; Chorghade, M. S.; Hecht, S. M. Biochemistry 1984, 23, 1468-1473.
10. (b) Heckler, T. G.; Chang, L.-H.; Zama, Y.; Naka, T.; Chorghade, M. S.; Hecht, S. M. Tetrahedron 1984, 40, 87-94.
11. The reaction mixture contained 55mM Hepes-KOH (pH 7.5), 210 mM potassium glutamate, 6.9 mM ammonium acetate, 9 mM magnesium acetate, 1.7 mM dithiothreitol, 1.2 mM ATP, 0.28 mM GTP, 26 mM phosphoenolpyruvate, 1 mM spermidine, 1.9% polyethyleneglycol-8000, 35 μg/mL folinic acid, 0.1 mM each of amino acids except arginine, 0.01 mM arginine, 4 μg of mRNA, and 2 μL of E. czoli S-30 extract in 10 μL. The tRNA charged with a nonnatural amino acid was added to 25 μM. The mixture was incubated at 37 °C for 60 min.
12. Bain, J. D.; Switzer, C.; Chamberlin, A. R.; Benner, S. A. Nature 1992, 356, 537-539.