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Volumn 32, Issue 4, 2004, Pages 258-262

Immunoassay for visualization of protein-protein interactions on Ni-nitrilotriacetate support: Example of a laboratory exercise with recombinant heterotrimeric Gαi2β1γ2 tagged by hexahistidine from sf9 cells

Author keywords

Education; G proteins; Immunobloting; Sf9 cells

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; HISTIDINE; NICKEL; NITRILOTRIACETIC ACID;

EID: 3242794875     PISSN: 14708175     EISSN: None     Source Type: Journal    
DOI: 10.1002/bmb.2004.494032040380     Document Type: Article
Times cited : (6)

References (11)
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    • Regulation of Gi and Go by mastoparan, related amphiphilic peptides, and hydrophobic amines. Mechanism and structural determinants of activity
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  • 8
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    • A. Bavec (2004) Novel features of amphiphilic peptide mas7 in signaling via heterotrimeric G-proteins, J. Pep. Sci., in press.
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    • Different role of intracellular loops of glucagon-like peptide-1 receptor in G-protein coupling
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  • 10
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    • Purification of recombinant G proteins from Sf9 cells by hexahistidine tagging of associated subunits. Characterization of α12 and inhibition of adenylyl cyclase by αz
    • T. Kozasa, A. G. Gilman (1995) Purification of recombinant G proteins from Sf9 cells by hexahistidine tagging of associated subunits. Characterization of α12 and inhibition of adenylyl cyclase by αz, J. Biol. Chem. 270, 1734-1741.
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.