Novel hairpin-shaped primer assay to study the association of the -44 single-nucleotide polymorphism of the DEFB1 gene with early-onset periodontal disease

Clinical and Diagnostic Laboratory Immunology

Volumn 11, Issue 4, 2004, Pages 766-769

  Boniotto, Michele ^a   Hazbón, Manzour Hernando ^b   Jordan, William James ^a   Lennon, Greig Patrick ^a   Eskdale, Joyce ^a   Alland, David ^b   Gallagher, Grant ^a,c  

^a UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY   (United States)

^b RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

^c RUTGERS SCHOOL OF DENTAL MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA DEFENSIN 1; CYTOSINE; DYE; FLUORESCENT DYE; GUANINE; PRIMER DNA; SYBR GREEN I; UNCLASSIFIED DRUG;

ACCURACY; AFRICAN AMERICAN; ANALYTIC METHOD; ARTICLE; CANDIDA; CAUCASIAN; CONTROLLED STUDY; DEFB1 GENE; DISEASE ASSOCIATION; DISEASE CARRIER; DNA DETERMINATION; DNA PROBE; EARLY ONSET PERIODONTAL DISEASE; ETHNIC GROUP; GENE; GENE AMPLIFICATION; HAIRPIN SHAPED PRIMER ASSAY; HUMAN; MAJOR CLINICAL STUDY; MOUTH MUCOSA; NUCLEOTIDE SEQUENCE; PERIODONTAL DISEASE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS DESIGN; PROCESS MONITORING; REAL TIME POLYMERASE CHAIN REACTION; SINGLE NUCLEOTIDE POLYMORPHISM;

ALLELES; BETA-DEFENSINS; DNA PRIMERS; ETHNIC GROUPS; GENOTYPE; HUMANS; PERIODONTAL DISEASES; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; SENSITIVITY AND SPECIFICITY;

EID: 3242723228     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/CDLI.11.4.766-769.2004     Document Type: Article

References (29)

1. Braida, L., M. Boniotto, A. Pontillo, P. A. Tovo, A. Amoroso, and S. Crovella. A single-nucleotide polymorphism (SNPs) in human beta-defensin 1 gene is associated with HIV-1 infection in Italian children. AIDS, in press.
2. Brissette, C. A., and S. A. Lukehart. 2002. Treponema denticola is resistant to human beta-defensins. Infect Immun. 70:3982-3984.
3. Cha, R. S., H. Zarbl, P. Keohavong, and W. G. Thilly. 1992. Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene. PCR Methods Appl. 2:14-20.
4. Doherty, T. M., L. A. Fitzpatrick, A. Shaheen, T. B. Rajavashisth, and R. C. Detrano. 2004. Genetic determinants of arterial calcification associated with atherosclerosis. Mayo Clin. Proc. 79:197-210.
5. Dunsche, A., Y. Acil, R. Siebert, J. Harder, J. M. Schroder, and S. Jepsen. 2001. Expression profile of human defensins and antimicrobial proteins in oral tissues. J. Oral Pathol. Med. 30:154-158.
6. Flavell, A. J., V. N. Bolshakov, A. Booth, R. Jing, J. Russell, T. H. Ellis, and P. Isaac. 2003. A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach. Nucleic Acids Res. 31:e115.
7. Germer, S., and R. Higuchi. 1999. Single-tube genotyping without oligonucleotide probes. Genome Res. 9:72-78.
8. Gray, I. C., D. A. Campbell, and N. K. Spurr. 2000. Single nucleotide polymorphisms as tools in human genetics. Hum. Mol. Genet. 9:2403-2408.
9. Hart, T. C. 1994. Genetic considerations of risk in human periodontal disease. Curr. Opin. Periodontol. 1994:3-11.
10. Hazbon, M. H., and D. Alland. 2004. Hairpin primers for simplified single-nucleotide polymorphism analysis of Mycobacterium tuberculosis and other organisms. J. Clin. Microbiol. 42:1236-1242.
11. Hladnik, U., L. Braida, M. Boniotto, D. Pirulli, F. Gerin, A. Amoroso, and S. Crovella. 2002. Single-tube genotyping of MBL-2 polymorphisms using melting temperature analysis. Clin. Exp. Med. 2:105-108.
12. Jurevic, R. J., M. Bai, R. B. Chadwick, T. C. White, and B. A. Dale. 2003. Single-nucleotide polymorphisms (SNPs) in human beta-defensin 1: high-throughput SNP assays and association with Candida carriage in type I diabetics and nondiabetic controls. J. Clin. Microbiol. 41:90-96.
13. Jurevic, R. J., P. Chrisman, L. Mancl, R. Livingston, and B. A. Dale. 2002. Single-nucleotide polymorphisms and haplotype analysis in beta-defensin genes in different ethnic populations. Genet. Test, 6:261-269.
14. Kaboev, O. K., L. A. Luchkina, A. N. Tret'iakov, and A. R. Bahrmand. 2000. PCR hot start using primers with the structure of molecular beacons (hairpin-like structure). Nucleic Acids Res. 28:E94.
15. Krisanaprakornkit, S., A. Weinberg, C. N. Perez, and B. A. Dale. 1998. Expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue. Infect. Immun. 66:4222-4228.
16. Lee, L. G., K. J. Livak, B. Mullah, R. J. Graham, R. S. Vinayak, and T. M. Woudenberg. 1999. Seven-color, homogeneous detection of six PCR products. BioTechniques 27:342-349.
17. Lipsky, R. H., C. M. Mazzanti, J. G. Rudolph, K. Xu, G. Vyas, D. Bozak, M. Q. Radel, and D. Goldman. 2001. DNA melting analysis for detection of single nucleotide polymorphisms. Clin. Chem. 47:635-644.
18. Mathews, M., H. P. Jia, J. M. Guthmiller, G. Losh, S. Graham, G. K. Johnson, B. F. Tack, and P. B. McCray, Jr. 1999. Production of beta-defensin antimicrobial peptides by the oral mucosa and salivary glands. Infect. Immun. 67:2740-2745.
19. Matsushita, I., K. Hasegawa, K. Nakata, K. Yasuda, K. Tokunaga, and N. Keicho. 2002. Genetic variants of human beta-defensin-1 and chronic obstructive pulmonary disease. Biochem. Biophys. Res. Commun. 291:17-22.
20. Meyer, K., A. Fredriksen, and P. M. Ueland. 2004. High-level multiplex genotyping of polymorphisms involved in folate or homocysteine metabolism by matrix-assisted laser desorption/ionization mass spectrometry. Clin. Chem. 50:391-402.
21. Michalowicz, B. S. 1994. Genetic and heritable risk factors in periodontal disease. J. Periodontol. 65:479-488.
22. Newton, C. R., A. Graham, L. E. Heptinstall, S. J. Powell, C. Summers, N. Kalsheker, J. C. Smith, and A. F. Markham. 1989. Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res. 17:2503-2516.
23. Ou-Yang, H., L. Hua, Q. H. Mo, and X. M. Xu. 2004. Rapid, accurate genotyping of the common-alpha(4.2) thalassaemia deletion based on the use of denaturing HPLC. J. Clin. Pathol. 57:159-163.
24. Putsep, K., G. Carlsson, H. G. Boman, and M. Andersson. 2002. Deficiency of antibacterial peptides in patients with morbus Kostmann: an observation study. Lancet 360:1144-1149.
25. Tyagi, S., D. P. Bratu, and F. R. Kramer. 1998. Multicolor molecular beacons for allele discrimination. Nat. Biotechnol. 16:49-53.
26. Tyagi, S., and F. R. Kramer. 1996. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14:303-308.
27. Wara-aswapati, N., T. H. Howell, H. L. Needleman, and N. Karimbux. 1999. Periodontitis in the child and adolescent. ASDC J. Dent. Child. 66:167-174, 154.
28. Waterfall, C. M., and B. D. Cobb. 2001. Single tube genotyping of sickle cell anaemia using PCR-based SNP analysis. Nucleic Acids Res. 29:e119.
29. Wu, D. Y., L. Ugozzoli, B. K. Pal, and R. B. Wallace. 1989. Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia. Proc. Natl. Acad. Sci. USA 86:2757-2760.