Use of bispecific antibodies in molecular velcro assays whose specificity approaches the theoretical limit of immunodetection for Bordetella peptussis

Clinical and Diagnostic Laboratory Immunology

Volumn 11, Issue 4, 2004, Pages 752-757

  Tang, X L ^a   Peppler, M S ^a   Irvin, R T ^a   Suresh, M R ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BENZOHYDROXAMIC ACID; BISPECIFIC ANTIBODY; HORSERADISH PEROXIDASE; IMMUNOGLOBULIN; MONOCLONAL ANTIBODY;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; BORDETELLA PERTUSSIS; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE ACTIVATED CELL SORTER; HYBRIDOMA; IMMUNODETECTION; MOLECULAR VELCRO ASSAY; MOUSE; NASOPHARYNX; NONHUMAN; PERTUSSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUADROMA; RAT; THROAT CULTURE;

ANIMALS; ANTIBODIES, BACTERIAL; ANTIBODIES, MONOCLONAL; BORDETELLA PERTUSSIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HORSERADISH PEROXIDASE; HYBRIDOMAS; SENSITIVITY AND SPECIFICITY;

EID: 3242702382     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/CDLI.11.4.752-757.2004     Document Type: Article

References (20)

1. Cao, Y., and M. R. Suresh. 1998. Bispecific antibodies as novel bioconjugates. Bioconj. Chem. 9:635-644.
2. Centers for Disease Control and Prevention. 2002. Pertussis 1997-2000. Morb. Mortal. Wkly. Rep. Surveill. Sum. 51:73-76.
3. 1H NMR investigation of the heme cavity and substrate binding site in cyanide-inhibited horseradish peroxidase. Biochemistry 38:1077-1086.
4. Ewanowich, C. A., L. W. Chui, M. G. Paranchych, M. S. Peppler, R. G. Marusyk, and W. L. Alhritton. 1993. Major outbreak of pertussis in northern Alberta, Canada: analysis of discrepant direct fluorescent-antibody and culture results by using polymerase chain reaction. J. Clin. Microbiol. 31:1715-1725.
5. Field, L. H., and C. D. Parker. 1977. Pertussis outbreak in Austin and Travis County. J. Clin. Microbiol. 6:154-160.
6. Gupta, S., and M. R. Suresh. 2002. Affinity chromatography and co-chromatography of bispecific monoclonal antibody immunoconjugates. J. Biochem. Biophys. 51:203-216.
7. Gustafsson, B., U. Lindquist, and M. Andersson. 1988. Production and characterization of monoclonal antibodies directed against Bordetella pertussis lipopolysaccharide. J. Clin. Microbiol. 26:188-193.
8. Hermanson, G. T. 1996. Bioconjugate techniques. Academic Press, Inc., San Diego, Calif.
9. Husereau, D. R., and M. R. Suresh. 2001. A general affinity method to purify peroxidase-tagged antibodies. J. Immunol. Methods 249:33-41.
10. Joseph, J. E. 2000. Pertussis in the adolescent and adult: a primary care concern. Clin. Excell. Nurse Pract. 4:361-365.
11. Karawajew, L., O. Behrsing, G. Kaiser, and B. Micheel. 1988. Production and ELISA application of bispecific monoclonal antibodies against fluorescein isothiocyanate (FITC) and horseradish peroxidase (HRP). J. Immunol. Methods 111:95-99.
12. Kreutz, F. T., D. Xu, and M. R. Suresh. 1998. A new method to generate quadromas by electrofusion and FACS sorting. Hybridoma 17:267-273.
13. Kricka, L. J. 1994. Selected strategies for improving sensitivity and reliability of immunoassays. Clin. Chem. 40:347-357.
14. McNicol, P., S. M. Glereke, M. Gray, D. Martin, B. Brodeur, M. S. Peppler, T. Williams, and G. Hammond. 1995. Evaluation and validation of a monoclonal immunofluorescence regent for direct detection of Bordetella pertussis. J. Clin. Microbiol. 33:2868-2871.
15. Milstein, C., and A. C. Cuello. 1983. Hybrid hybridomas and their use in immunohistochemistry. Nature 305:537-540.
16. Parker, C. D., and B. J. Payne. 1985. Bordetella pertussis, p. 394-399. In E. H. Lennette, A. Balows, W. J. Hausler, Jr., and H. J. Shadomy (ed.), Manual of clinical microbiology, 4th ed. American Society for Microbiology, Washington, D.C.
17. Peppler, M. S. 1984. Two physically and serologically distinct lipopolysaccharide profiles in strains of Bordetella pertussis and their phenotype variants. Infect. Immun. 43:224-232.
18. Stratieva-Taneeva, P. A., S. V. Khaidukov, V. A. Kovalenko, I. V. Nazimov, L. V. Samokhvalova, and V. A. Nesmeyanov. 1993. Bispecific monoclonal antibodies to human interleukin 2 and horseradish peroxidase. Hybridoma 12:271-284.
19. Suresh, M. R., A. C. Cuello, and C. Milstein. 1986. Advantages of bispecific hybridomas in one step immunocytochemistry and immunoassays. Proc. Natl. Acad. Sci. USA 83:7989-7993.
20. Tilley, P. A., M. V. Kanchana, I. Knight, J. Biondeau, N. Antonishyn, and H. Deneer. 2000. Detection of Bordetella pertussis in a clinical laboratory by culture, polymerase chain reaction and direct fluorescence antibody staining; accuracy, and cost. Diagn. Microbiol. Infect. Dis. 37:17-23.