A general affinity method to purify peroxidase-tagged antibodies

Journal of Immunological Methods

Volumn 249, Issue 1-2, 2001, Pages 33-41

  Husereau, Donald R ^a   Suresh, Mavanur R ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Affinity chromatography; Antibodies; Bispecific antibodies; HRPO

Indexed keywords

AGAROSE; AMMONIUM SULFATE; BENZOHYDROXAMIC ACID; BISPECIFIC ANTIBODY; BORIC ACID; BUFFER; HORSERADISH PEROXIDASE; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; PROTEIN ANTIBODY; PROTEIN P57; STREPTAVIDIN;

ANTIBODY AFFINITY; ANTIBODY LABELING; ANTIGEN ANTIBODY COMPLEX; ARTICLE; DIALYSIS; HYBRID CELL; PH; PRECIPITATION; PRIORITY JOURNAL; PROTEIN PURIFICATION; SUPERNATANT;

ANIMALS; ANTIBODIES; CHROMATOGRAPHY, AFFINITY; HUMANS; IMMUNOENZYME TECHNIQUES; PEROXIDASE;

ARMORACIA RUSTICANA;

EID: 0035282667     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00277-5     Document Type: Article

References (24)

1. Purification of peroxidase-conjugated antibody for enzyme immunoassay by affinity chromatography on concanavalin A
2. The interaction of benzhydroxamic acid with horseradish peroxidase and its fluorescent analogs
3. Development of a bispecific monoclonal antibody as a universal immunoprobe for detecting biotinylated macromolecules
4. Bispecific antibodies as novel bioconjugates
5. Targeted killing of yeast expressing a HIV-1 peptide by antibody-conjugated glucose oxidase and horseradish peroxidase
6. 1H NMR investigation of the heme cavity and substrate binding site in cyanide-inhibited horseradish peroxidase
7. Immunoassay and other ligand assays: Present status and future trends
8. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G
9. Structural interactions between horseradish peroxidase C and the substrate benzhydroxamic acid determined by X-ray crystallography
10. Epitope mapping of prostate-specific antigen with monoclonal antibodies
11. Novel bispecific immunoprobe for rapid and sensitive detection of prostate-specific antigen
12. Efficient bispecific monoclonal antibody purification using gradient thiophilic affinity chromatography
13. Selected strategies for improving sensitivity and reliability of immunoassays
14. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
15. Single-step purification of bispecific monoclonal antibodies for immunotherapeutic use by hydrophobic interaction chromatography
16. Overview of antibody use in immunocytochemistry
17. Hybrid hybridomas and their use in immunohistochemistry
18. Enzyme-immunoassay: A review
19. Purification of plant peroxidases by affinity chromatography
20. Concomitant purification of prostatic carcinoma tumor markers from human seminal fluid under nondenaturing conditions
21. Bispecific monoclonal antibodies from hybrid hybridomas
22. The use of methyl-substituted benzhydroxamic acids as structural probes of peroxidase substrate binding
23. Mimetic ligand-based affinity purification of immune complexes and immunoconjugates