Development of an optimized protocol for the detection of classical swine fever virus in formalin-fixed, paraffin-embedded tissues by seminested reverse transcription-polymerase chain reaction and comparison with in situ hybridization

Research in Veterinary Science

Volumn 77, Issue 2, 2004, Pages 163-169

  Ha, S K ^a   Choi, C ^a   Chae, C ^a  

^a Seoul National University   (South Korea)

Author keywords

Classical swine fever virus; Diagnosis; Formalin fixed tissue; Polymerase chain reaction

Indexed keywords

NUCLEIC ACID; PROTEINASE K;

AMPLICON; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BASE PAIRING; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; ENZYME DEGRADATION; EXPERIMENTAL INFECTION; EXTRACTION; IN SITU HYBRIDIZATION; INTERMETHOD COMPARISON; LYMPH NODE; NONHUMAN; PESTIVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SWINE DISEASE; TISSUE SECTION; VIRUS DETECTION; VIRUS INFECTION;

ANIMALS; CLASSICAL SWINE FEVER; CLASSICAL SWINE FEVER VIRUS; DNA PRIMERS; ENDOPEPTIDASE K; FIXATIVES; FORMALDEHYDE; GUANIDINES; IN SITU HYBRIDIZATION; LYMPH NODES; PARAFFIN EMBEDDING; PHENOLS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SWINE; TISSUE FIXATION; TRANSCRIPTION, GENETIC;

CLASSICAL SWINE FEVER VIRUS; SUS SCROFA;

EID: 3242686398     PISSN: 00345288     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.rvsc.2004.03.006     Document Type: Article

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