Differentiation between porcine epidemic diarrhea virus and transmissible gastroenteritis virus in formalin-fixed paraffin-embedded tissues by multiplex RT-nested PCR and comparison with in situ hybridization

Journal of Virological Methods

Volumn 108, Issue 1, 2003, Pages 41-47

  Jung, Kwonil ^a   Kim, Junghyum ^a   Kim, Okjin ^a   Kim, Bongtae ^a   Chae, Chanhee ^a  

^a Seoul National University   (South Korea)

Author keywords

Formalin fixed tissue; In situ hybridization; Polymerase chain reaction; Porcine epidemic diarrhea virus; RNA extraction; Transmissible gastroenteritis virus

Indexed keywords

COMPLEMENTARY DNA; FORMALDEHYDE; PARAFFIN; PROTEINASE K; VIRUS DNA; VIRUS RNA; XYLENE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DNA DETERMINATION; EMBEDDING; ENTERIC VIRUS; IN SITU HYBRIDIZATION; INTERMETHOD COMPARISON; JEJUNUM; NONHUMAN; PORCINE EPIDEMIC DIARRHEA VIRUS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SWINE DISEASE; TISSUE FIXATION; TRANSMISSIBLE GASTROENTERITIS VIRUS; VIRUS IDENTIFICATION; VIRUS INFECTION;

ANIMALS; BASE SEQUENCE; CORONAVIRUS; DNA PRIMERS; DNA, VIRAL; EPIDEMIOLOGY, MOLECULAR; FORMALDEHYDE; GASTROENTERITIS, TRANSMISSIBLE, OF SWINE; IN SITU HYBRIDIZATION; JEJUNUM; PARAFFIN EMBEDDING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SUS SCROFA; TISSUE FIXATION; TRANSMISSIBLE GASTROENTERITIS VIRUS; VIRAL MATRIX PROTEINS; VIROLOGY;

PORCINE EPIDEMIC DIARRHEA VIRUS; SUIDAE; TRANSMISSIBLE GASTROENTERITIS VIRUS;

EID: 0037360464     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(02)00253-7     Document Type: Article

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