Assay of Rab4-dependent trafficking on microtubules

Methods in Enzymology

Volumn 403, Issue , 2005, Pages 92-107

  Murray, John W ^a   Wolkoff, Allan W ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RABAPTIN 4; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; CELL ORGANELLE; CELL TYPE; CELL VACUOLE; CONFOCAL MICROSCOPY; ENDOCYTOSIS; ENDOSOME; EPIFLUORESCENCE MICROSCOPE; FISSION NEUTRON; FIXATION FAILURE; FLUORESCENCE; IMMUNOFLUORESCENCE; LIVER; MEMBRANE TRANSPORT; MICROSCOPY; MICROSOME; MICROTUBULE; NONHUMAN; PARAMETER; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; QUANTITATIVE ANALYSIS; REVIEW; TECHNIQUE; TRANSPORT KINETICS; VELOCITY;

ANIMALS; ENDOSOMES; FLUORESCENT DYES; LIVER; MICROTUBULES; PROTEIN TRANSPORT; RAB4 GTP-BINDING PROTEINS; RATS; RATS, SPRAGUE-DAWLEY;

EID: 32344446788     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(05)03009-0     Document Type: Review

References (19)

1. Bananis E., Murray J.W., Stockert R.J., Satir P., and Wolkoff A.W. Microtubule and motor-dependent endocytic vesicle sorting in vitro J. Cell Biol. 151 2000 179 186
2. Bananis E., Murray J.W., Stockert R.J., Satir P., and Wolkoff A.W. Regulation of early endocytic vesicle motility and fission in a reconstituted system J. Cell Sci. 116 2003 2749 2761
3. Bananis E., Nath S., Gordon K., Satir P., Stockert R.J., Murray J.W., and Wolkoff A.W. Microtubule-dependent movement of late endocytic vesicles in vitro: Requirements for dynein and kinesin Mol. Biol. Cell 15 2004 3688 3697
4. Blocker A., Severin F.F., Burkhardt J.K., Bingham J.B., Yu H., Olivo J.C., Schroer T.A., Hyman A.A., and Griffiths G. Molecular requirements for bi-directional movement of phagosomes along microtubules J. Cell Biol. 137 1997 113 129
5. Higuchi H., and Endow S.A. Directionality and processivity of molecular motors Curr. Opin. Cell. Biol. 14 2002 50 57
6. Howard J., and Hyman A.A. Preparation of marked microtubules for the assay of the polarity of microtubule-based motors by fluorescence microscopy Methods Cell Biol. 39 1993 105 113
7. Inoue S. Video Microscopy 1986 Plenum New York
8. Kishino A., and Yanagida T. Force measurements by micromanipulation of a single action filament by glass needles Nature 334 1988 74 76
9. McCaffrey M.W., Bielli A., Cantalupo G., Mora S., Roberti V., Santillo M., Drummond F., and Bucci C. Rab4 affects both recycling and degradative endosomal trafficking FEBS Lett. 495 2001 21 30
10. Murray J.W., Bananis E., and Wolkoff A.W. Reconstitution of ATP-dependent movement of endocytic vesicles along microtubules in vitro: An oscillatory bidirectional process Mol. Biol. Cell 11 2000 419 433
11. Murray J.W., Bananis E., and Wolkoff A.W. Immunofluorescence microchamber technique for characterizing isolated organelles Anal. Biochem. 305 2002 55 67
12. Muto E., Sakai H., and Kaseda K. Long-range cooperative binding of kinesin to a microtubule in the presence of ATP J. Cell Biol. 168 2005 691 696
13. Pollock N., de Hostos E.L., Turck C.W., and Vale R.D. Reconstitution of membrane transport powered by a novel dimeric kinesin motor of the Unc104/KIF1A family purified from Dictyostelium J. Cell Biol. 147 1999 493 506
14. Sheff D.R., Daro E.A., Hull M., and Mellman I. The receptor recycling pathway contains two distinct populations of early endosomes with different sorting functions J. Cell Biol. 145 1999 123 139
15. Stockert R.J. The asialoglycoprotein receptor: Relationships between structure, function, and expression Physiol Rev. 75 1995 591 609
16. van der Sluijs P., Mohrmann K., Deneka M., and Jongeneelen M. Expression and properties of Rab4 and its effector rabaptin-4 in endocytic recycling Methods Enzymol. 329 2001 111 119
17. Wang Z., and Sheetz M.P. The C-terminus of tubulin increases cytoplasmic dynein and kinesin processivity Biophys. J. 78 2000 1955 1964
18. Waterman-Storer C.M. Microtubule/organelle motility assays J.S. Bonifacino M. Dasso J.B. Harford J. Lippincott-Schwartz K.M. Yamada "Current Protocols in Cell Biology" Vol. Unit 13.1 1998 John Wiley New York
19. Weigel P.H. Characterization of the asialoglycoprotein receptor on isolated rat hepatocytes J. Biol. Chem. 255 1980 6111 6120