Human villous trophoblast and the lack of intron 4-retaining soluble HLA-G secretion: Beware of possible methodological biases

Molecular Human Reproduction

Volumn 11, Issue 10, 2005, Pages 711-713

  Le Bouteiller, Philippe ^a  

^a CHU PURPAN   (France)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; HLA G ANTIGEN; RECOMBINANT PROTEIN;

ANTIGEN EXPRESSION; CELL CULTURE; CHORION VILLUS; CONTROLLED STUDY; CROSS REACTION; CYTOTROPHOBLAST; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INTRON; NOTE; PLACENTA DEVELOPMENT; PRIORITY JOURNAL; PROTEIN SECRETION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SYSTEMATIC ERROR; TROPHOBLAST; WESTERN BLOTTING;

CELL LINE; CHORIONIC VILLI; FEMALE; HISTOCOMPATIBILITY ANTIGENS CLASS I; HLA ANTIGENS; HUMANS; PREGNANCY; PROTEIN ISOFORMS; RESEARCH DESIGN; TROPHOBLASTS;

EID: 32144460803     PISSN: 13609947     EISSN: 14602407     Source Type: Journal    
DOI: 10.1093/molehr/gah211     Document Type: Note

References (11)

1. Astrid Blaschitz, Herbert Juch, Armin Volz, Heinz Hutter, Christine Daxboeck, Gernot Desoye, Gottried Dohr (2005) The soluble pool of HLA-G produced by human human trophoblast does not contain the intron 4-derived peptide. Mol Hum Reprod 00,00-00.
2. Barel M, Ressing M, Pizzato N, van Leeuwen D, Le Bouteiller P, Lenfant F and Wiertz E (2003) Human cytomegalovirus-encoded US2 differentially affects surface expression of MHC class I locus products and targets membrane bound, but not soluble HLA-G1 for degradation. J Immunol 171,6757-6765.
3. Chu W, Fant ME, Geraghty DE and Hunt JS (1998) Soluble HLA-G in human placentas: Synthesis in trophoblasts and interferon-gamma-activated macrophages but not placental fibroblasts. Hum Immunol 59,435-442.
4. Ishitani A and Geraghty DE (1992) Alternative splicing of HLA-G transcripts yields proteins with primary structures resembling both class I and class II antigens. Proc Natl Acad Sci USA 89,3947-3951.
5. Ishitani A, Sageshima N, Lee N, Dorofeeva N, Hatake K, Marquardt H and Geraghty D (2003) Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition. J Immunol 171,1376-1384.
6. Kohler P and Bridson W (1971) Isolation of hormone-producing cloned lines of human choriocarcinoma. J Clin Endocrinol Metab 32,683-687.
7. Langat DK, Morales PJ, Fazleabas AT, Mwenda JM and Hunt JS (2002) Baboon placentas express soluble and membrane-bound Paan-AG proteins encoded by alternatively spliced transcripts of the class Ib major histocompatibility complex gene, Paan-AG. Immunogenetics 54,164-173.
8. Morales P, Pace J, Platt J, Phillips T, Morgan K, Fazleabas A and Hunt J (2003) Placental cell expression of HLA-G2 isoforms is limited to the invasive trophoblast phenotype. J Immunol 171,6215-6224.
9. Ryan AF, Grendell RL, Geraghty DE and Golos TG (2002) A soluble isoform of the rhesus monkey nonclassical MHC class I molecule Mamu-AG is expressed in the placenta and the testis. J Immunol 169,673-683.
10. Slukvin II, Boyson JE, Watkins DI and Golos TG (1998) The rhesus monkey analogue of human lymphocyte antigen-G is expressed primarily in villous syncytiotrophoblasts. Biol Reprod 58,728-738.
11. Solier C, Aguerre-Girr M, Lenfant F, Campan A, Berrebi A, Rebmann V, Grosse-Wilde H and Le Bouteiller P (2002) Secretion of pro-apoptotic intron 4-retaining soluble HLA-G1 by human villous trophoblasts. Eur Immunol 32,3576-3586