A simple method for unbiased quantitation of adoptively transferred cells in solid tissues

Journal of Immunological Methods

Volumn 309, Issue 1-2, 2006, Pages 173-181

  Petersen, M S ^a   Petersen, C C ^a   Agger, R ^a   Hokland, M ^a   Gundersen, H J G ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

Cell quantitation; CFSE; Microscopy; Optical fractionator

Indexed keywords

CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER;

ADOPTIVE TRANSFER; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL LOSS; CELL TRANSFER; CONTROLLED STUDY; FEMALE; FLUORESCENCE MICROSCOPY; MOUSE; NONHUMAN; ORGAN DISTRIBUTION; PRIORITY JOURNAL; SPLEEN CELL; TISSUE DISTRIBUTION;

ADOPTIVE TRANSFER; ANIMALS; BIOMETRY; CELL COUNT; CELL SEPARATION; FEMALE; FLUORESCEINS; FLUORESCENT DYES; IMMUNOLOGIC TECHNIQUES; MICE; MICE, INBRED C57BL; MICROSCOPY, FLUORESCENCE; ORGAN SPECIFICITY; SPLEEN; SUCCINIMIDES; TIME FACTORS;

ANIMALIA;

EID: 31944443968     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2005.12.002     Document Type: Article

References (11)

1. P.H. Basse Tissue distribution and tumor localization of effector cells in adoptive immunotherapy of cancer APMIS Suppl. 55 1995 1
2. P. Basse, P. Hokland, and M. Hokland Comparison between 125IUdR and 51Cr as cell labels in investigations of tumor cell migration Int. J. Radiat. Appl. Instrum., B 17 1990 781
3. S. Gross, and D. Piwnica-Worms Spying on cancer: molecular imaging in vivo with genetically encoded reporters Cancer Cell 7 2005 5
4. H.J. Gundersen Notes on the estimation of the numerical density of arbitrary profiles, the edge effect J. Microsc. 111 1977 219
5. H.J. Gundersen Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson J. Microsc. 143 Pt. 1 1986 3
6. H.J. Gundersen, E.B. Jensen, K. Kieu, and J. Nielsen The efficiency of systematic sampling in stereology-reconsidered J. Microsc. 193 1999 199
7. J. Hasbold, and P.D. Hodgkin Flow cytometric cell division tracking using nuclei Cytometry 40 2000 230
8. M.F. Kircher, J.R. Allport, E.E. Graves, V. Love, L. Josephson, A.H. Lichtman, and R. Weissleder In vivo high resolution three-dimensional imaging of antigen-specific cytotoxic T-lymphocyte trafficking to tumors Cancer Res. 63 2003 6838
9. A.A. Maghazachi, and L. Fitzgibbon Fate of intravenously administered rat lymphokine-activated killer cells labeled with different markers Cancer Immunol. Immunother. 31 1990 139
10. C.R. Parish Fluorescent dyes for lymphocyte migration and proliferation studies Immunol. Cell Biol. 77 1999 499
11. M.J. West, L. Slomianka, and H.J. Gundersen Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator Anat. Rec. 231 1991 482