Flow cytometric cell division tracking using nuclei

Cytometry

Volumn 40, Issue 3, 2000, Pages 230-237

  Hasbold, Jhagvaral ^a,b   Hodgkin, Philip D ^b  

^a CENTENARY INSTITUTE   (Australia)

^b UNIVERSITY OF SYDNEY   (Australia)

Author keywords

BrdU; CFSE; Flow cytometry; Nuclei

Indexed keywords

6 CARBOXYFLUORESCEIN; BROXURIDINE; CELL DNA; CYCLINE; DACTINOMYCIN; PARAFORMALDEHYDE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL CYCLE; CELL DIVISION; CELL LABELING; CELL NUCLEUS; CONTROLLED STUDY; CYTOLYSIS; FLOW CYTOMETRY; IMMUNOFLUORESCENCE; MOUSE; NONHUMAN; PRIORITY JOURNAL; STAINING;

ANIMALS; BROMODEOXYURIDINE; CELL DIVISION; CELL FRACTIONATION; CELL NUCLEUS; DACTINOMYCIN; FEMALE; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; MALE; MICE; MICE, INBRED CBA; PROLIFERATING CELL NUCLEAR ANTIGEN; STAINING AND LABELING; SUCCINIMIDES;

ANIMALIA;

EID: 0033937116     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20000701)40:3<230::AID-CYTO8>3.0.CO;2-E     Document Type: Article

References (21)

1. Lebman DA, Coffman RL. Interleukin 4 causes isotype switching to IgE in T cell-stimulated clonal B cell cultures. J Exp Med 1988;168: 853-862.
2. Noelle RJ, Ledbetter JA, Aruffo A. CD40 and its ligand, an essential ligand-receptor pair for thymus-dependent B-cell activation, Immunol Today 1992;13:431-433.
3. Kehry MR, Castle BE. Regulation of CD40 ligand expression and use of recomhinant CD40 ligand for studying B cell growth and differentiation. Semin Immunol 1994;6:287-294.
4. Lyons AB, Parish CR. Determination of lymphocyte division by flow cytometry. J Immunol Methods 1994;171:131-137.
5. Lyons AB, Hashold J, Hodgkin PD. Flow cytometric analysis of cell division history using dilution of CFSE, a stably integrated fluorescent probe. Methods Cell Biol (in press).
6. Hodgkin PD, Lee JH, Lyons AB. B cell differentiation and isotype switching is related to division cycle number. J Exp Med 1996;184: 277-281.
7. Hasbold J, Lyons AB, Kehry MR, Hodgkin PD. Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL-4. Eur J Immunol 1998;28:1040-1051.
8. Deenick EK, Hasbold J, Hodgkin PD. Switching to IgG3, IgG2b, and IgA is division linked and independent, revealing a stochastic framework for describing differentiation. J Immunol 1999;163:4707-4714.
9. Hasbold J, Hong JS-Y, Kehry MR, Hodgkin PD. Integrating signals from IFN-g and IL-4 by B cells: positive and negative effects on CD40 ligand-induced proliferation, survival, and division-linked isotype switching to IgGI1, IgE, and IgG2a. J Immunol 1999;163:4175-4181.
10. Hodgkin PD, Deenick E, Gett A, Avery D, Hong J, Rush J, McCall M, Hasbold J. Differentiation of lymphocytes is regulated by division number. In: Talwar GP, Nath I, Ganguly NK, Rao KVS, editors. Proceedings of the 10th International Congress of Immunology, New Delhi, India. Bologna, Italy: Monduzzi Editore; 1998. p 203-209.
11. Gett AV, Hodgkin PD. Cell division regulates the T cell cytokine repertoire, revealing a mechanism underlying immune class regulation. Proc Nail Acad Sci USA 1998;95:9488-9493.
12. Hodgkin PD, Kehry MR. Methods for polydonal B lymphocyte activation to proliferation and Ig secretion in vitro. In: Herzenberg LA, Weir DM, Blackwell C, editors. Weir's handbook of experimental immunology. Oxford: Blackwell Science; 1996 VIII, chapt 89.
13. Lanier LL, Warner NL. Paraformaldehyde fixation of hemalopoetic cells for quantitative flow cytometry (FACS) analysis. J Immunol Methods 1981;47;25-30.
14. Zarbo RJ, Visscher DW, Crissman JD. Two color multiparametric method for flow cytometric DNA analysis of carcinomas using staining for cytokeratin and leukocyte-common antigen. Anal Quant Cytol Histol 1989;11:391-402.
15. Darzynkiewicz Z., Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P. Traganos F. Features of apoptotic cells measured by flow cytometry. Cytometry 1992;13:795-808.
16. Carayon P, Bord A. Identification of DNA-replicating lymphocyte subsets using a new method to label the bromo-deoxyuridine incorporated into the DNA. J Immunol Methods 1992;147:225-230.
17. Mathews MB, Hernstein RM, Franza BR Jr, Garrells JI. Identity of the proliferating cell nuclear antigen and cyclin. Nature 1984;309:374-376.
18. Leonard WJ, O'Shea JJ, JAKs and STATs: biological implications. Annu Rev Immunol 1998;16:293-322.
19. Haugland RP. Handbook of fluorescent probes and research chemicals. Eugene, OR: Molecular Probes, Inc.; 1996. 680 p.
20. Blobel G, Potter VR. Nuclei from rat liver: isolation method that combines purity with high yield. Science 1966;154:1662-1665.
21. Johnston IR, Mathias AP. Pennington F, Ridge D. The fractionation of nuclei from mammalian cells by zonal centrifugation. Biochem J 1968;109:127-135.