Multiplex PCR assay for detection of Streptococcus suis species and serotypes 2 and 1/2 in tonsils of live and dead pigs

Journal of Clinical Microbiology

Volumn 42, Issue 7, 2004, Pages 3169-3175

  Marois, C ^a   Bougeard, S ^a   Gottschalk, M ^b   Kobisch, M ^a  

^a Healthy Pigs Production and Experimentation Section   (France)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ACCURACY; ADOLESCENT; ANIMAL TISSUE; ARTICLE; BACTERIAL MEMBRANE; BACTERIUM CARRIER; BACTERIUM DETECTION; COLONY FORMING UNIT; CONTROL; CPS2J GENE; EXPERIMENTAL INFECTION; GENE AMPLIFICATION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA 16S GENE; RNA GENE; SAMPLE; SEROTYPE; STREPTOCOCCUS SUIS; STREPTOCOCCUS SUIS SEROTYPE 1; STREPTOCOCCUS SUIS SEROTYPE 1 2; STREPTOCOCCUS SUIS SEROTYPE 2; STREPTOCOCCUS SUIS SEROTYPE 3; SWINE; TONSIL;

ANIMALS; ENZYME-LINKED IMMUNOSORBENT ASSAY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPING; STREPTOCOCCUS SUIS; SWINE; TONSIL;

ANIMALIA; BACTERIA (MICROORGANISMS); POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS SUIS; SUS SCROFA;

EID: 3142707225     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.7.3169-3175.2004     Document Type: Article

References (31)

1. Baele, M., V. Storms, F. Haesebrouck, L. A. Devriese, M. Gillis, G. Verschraegen, T. de Baere, and M. Vaneechoutte. 2001. Application and evaluation of the interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) for identification of Streptococcus species. J. Clin. Microbiol. 39:1436-1442.
2. Bensaid, T., B. Bonnefoi-Kyrlacou, C. Dupel-Pottier, O. Bellon, E. Lagier, and H. Chardon. 2003. Streptococcus suis meningitis following wild boar hunting. Presse Med. 32:1077-1078.
3. Berthelot-Hérault, F., R. Cariolet, A. Labbe, M. Gottschalk, J. Y. Cardinal, and M. Kobisch. 2001. Experimental infection of specific pathogen free piglets with French strains of Streptococcus suis capsular type 2. Can. J. Vet. Res. 65:196-200.
4. Berthelot-Hérault, F., M. Gottschalk, A. Labbe, R. Cariolet, and M. Kobisch. 2001. Experimental airborne transmission of Streptococcus suis capsular type 2 in pigs. Vet. Microbiol. 82:69-80.
5. Berthelot-Hérault, F., H. Morvan, A. M. Kéribin, M. Gottschalk, and M. Kobisch. 2000. Production of muramidase released protein (MRP), extracellular factor (EF) and haemolysin by field isolates of Streptococcus suis capsular type 2, 1/2, 9, 7 and 3 isolated from swine in France. Vet. Res. 31:473-479.
6. Boye, M., A. A. Feenstra, C. Tegtmeier, L. O. Andresen, S. R. Rasmussen, and V. Bille-Hansen. 2000. Detection of Streptococcus suis by in situ hybridization, indirect immunofluorescence, and peroxidase-antiperoxidase assays in formalin-fixed, paraffin-embedded tissue sections from pigs. J. Vet. Diagn. Investig. 12:224-232.
7. Cariolet, R., P. Marie, G. Moreau, and H. Robert. 1994. Rappel des différentes méthodes d'obtention de porcelets assainis: conditions de maintien du statut sanitaire et valorisation de ces animaux. Journées Rech, Porcine France 26:1-12.
8. Chatellier, S., J. Harel, Y. Zhang, M. Gottschalk, R. Higgins, L. A. Devriese, and R. Brousseau. 1998. Phylogenetic diversity of Streptococcus suis strains of various serotypes as revealed by 16S rRNA gene sequence comparison. Int. J. Syst. Bacteriol. 48:581-589.
9. Cloutier, G., S. D'Allaire, G. Martinez, C. Surprenant, S. Lacouture, and M. Gottschalk. 2003. Epidemiology of Streptococcus suis serotype 5 infection in a pig herd with and without clinical disease. Vet. Microbiol. 97:135-151.
10. Durand, F., C. L. Périno, C. Recule, J. P. Brion, M. Kobisch, F. Guerber, and J. Croizé. 2001. Bacteriological diagnosis of Streptococcus suis meningitis. Eur. J. Clin. Microbiol. 20:519-521.
11. Fittipaldi, N., A. Broes, J. Harel, M. Kobisch, and M. Gottschalk. 2003. Evaluation and field validation of PCR tests for detection of Actinobacillus pleuropneumoniae in subclinically infected pigs. J. Clin. Microbiol. 41:5085-5093.
12. François, B., V. Gissot, M. C. Ploy, and P. Vignon. 1998. Recurrent septic shock due to Streptococcus suis. J. Clin. Microbiol. 36:2395.
13. Friis, N. F. 1975. Some recommendations concerning primary isolation of Mycoplasma suispneumoniae and Mycoplasma flocculare: a survey. Nord. Vet. Med. 27:337-339.
14. Gottschalk, M., R. Higgins, M. Jacques, K. R. Mittal, and J. Henrichsen. 1989. Description of 14 new capsular types of Streptococcus suis. J. Clin. Microbiol. 27:2633-2636.
15. Higgins, R., and M. Gottschalk. 1999. Streptococcal diseases, p. 563-578. In B. E. Straw, S. D'Allaire, W. L. Mengeling, and D. J. Taylor (ed.), Diseases of swine, 8th ed. Iowa University Press, Ames.
16. Kellog, D. E., and S. Kwok. 1990. Detection of human immunodeficiency virus, p. 339-343. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (ed.), PCR protocols: a guide to methods and applications. Academic Press, San Diego, Calif.
17. Le Minor, L., and M. Véron. 1990. Bactériologie médicale. Médecine-Sciences, Flammarion, Paris, France.
18. Matsuo, H., and S. Sakamoto. 2003. Purulent meningitis caused by Streptococcus suis in a pig breeder. Kansenshogaku Zasshi 77:340-342.
19. Okwumabua, O., O. Abdelmagid, and M. M. Chengappa. 1999. Hybridization analysis of the gene encoding a hemolysin (suilysin) of Streptococcus suis type 2: evidence for the absence of the gene in some isolates. FEMS Microbiol. Lett. 181:113-121.
20. Okwumabua, O., M. O'Connor, and E. Shull. 2003. A polymerase chain reaction (PCR) assay specific for Streptococcus suis based on the gene encoding the glutamate dehydrogenase. FEMS Microbiol. Lett. 218:79-84.
21. Pedroll, S., M. Kobisch, O. Beauchet, J. P. Chaussinand, and F. Lucht. 2003. Streptococcus suis bacteriemia. Presse Med. 32:599-601.
22. Rosenkranz, M, H. A. Elsner, H. J. Sturenburg, C. Weiller, J. Rother, and I. Sobottka. 2003. Streptococcus suis meningitis and septicemia contracted from a wild boar in Germany. J. Neurol. 250:869-870.
23. Smith, H. E., M. Damman, J. van der Velde, F. Wagenaar, H. J. Wisselink, N. Stockhofe-Zurwieden, and M. A. Smits. 1999. Identification and characterization of the cps locus of Streptococcus suis serotype 2: the capsule protects against phagocytosis and is an important virulence factor. Infect. Immun. 67:1750-1756.
24. Smith, H. E., R. de Vries, R. van't Slot, and M. A. Smits. 2000. The cps locus of Streptococcus suis serotype 2: genetic determinant for the synthesis of sialic acid. Microb. Pathog. 29:127-134.
25. Smith, H. E., L. van Bruijnsvoort, H. Buijs, H. J. Wisselink, and M. A. Smits. 1999. Rapid PCR test for Streptococcus suis serotype 7. FEMS Microbiol. Lett. 178:265-270.
26. Smith, H. E., V. Veenbergen, J. van der Velde, M. Damman, H. J. Wisselink, and M. A. Smits. 1999. The cps genes of Streptococcus suis serotypes 1, 2, and 9: development of rapid serotype-specific PCR assays. J. Clin. Microbiol. 37:3146-3151
27. Staats, J. J., I. Feder, O. Okwumabua, and M. M. Chengappa. 1997. Streptococcus suis: past and present. Vet. Res. Commun. 21:381-387.
28. Strangmann, E., H. Froleke, and K. P. Kohse. 2002. Septic shock caused by Streptococcus suis: case report and investigation of a risk group. Int. J. Hyg. Environ. Health. 205:385-392.
29. Trottier, S., R. Higgins, G. Brochu, and M. Gottschalk. 1991. A case of human endocarditis due to Streptococcus suis in North America. Rev. Infect. Dis. 13:1251-1252.
30. Wisselink, H. J., F. H. Reek, U. Vecht, N. Stockhofe-Zurwieden, M. A. Smits, and H. E. Smith. 1999. Detection of virulent strains of Streptococcus suis type 2 and highly virulent strains of Streptococcus suis type 1 in tonsillar specimens of pigs by PCR. Vet. Microbiol. 67:143-157.
31. Wisselink, H. J., J. J. Joosten, and H. E. Smith. 2002. Multiplex PCR assays for simultaneous detection of six major serotypes and two virulence-associated phenotypes of Streptococcus suis in tonsillar specimens from pigs. J. Clin. Microbiol. 40:2922-2929.