Multiplex PCR assays for simultaneous detection of six major serotypes and two virulence-associated phenotypes of Streptococcus suis in tonsillar specimens from pigs

Journal of Clinical Microbiology

Volumn 40, Issue 8, 2002, Pages 2922-2929

  Wisselink, Henk J ^a   Joosten, Jeroen J ^a   Smith, Hilde E ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL POLYSACCHARIDE; BACTERIAL PROTEIN; PROTEIN EF; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL VIRULENCE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; GENE AMPLIFICATION; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEROTYPE; STREPTOCOCCUS SUIS; SWINE; TONSIL;

ANIMALS; DNA, BACTERIAL; PHENOTYPE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPING; STREPTOCOCCAL INFECTIONS; STREPTOCOCCUS SUIS; SWINE DISEASES; TONSIL; VIRULENCE;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS SUIS; SUS SCROFA;

EID: 0036702046     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.40.8.2922-2929.2002     Document Type: Article

References (29)

1. Allgaier, A., R. Goethe, H. J. Wisselink, H. E. Smith, and P. Valentin Weigand. 2001. Relatedness of Streptococcus suis isolates of various sero types and clinical backgrounds as evaluated by macrorestriction analysis and expression of potential virulence traits. J. Clin. Microbiol 39:445-453.
2. Arends, J. P., N. Hartwig, M. Rudolphy, and H. C. Zanen. 1984. Carrier rate of Streptococcus suis capsular type 2 in palatine tonsils of slaughtered pigs. J. Clin. Microbiol. 20:945-947.
3. Chengappa, M. M., L. W. Pace, J. A. Williams, C. H. Herren, and S. E. Ascher. 1990, Efficacy of tiamulin against experimentally induced Streptococ cus suis type-2 infection in swine, J. Am. Vet. Med. Assoc. 197:1467-1470.
4. Devriese L. A., K. Ceyssens, J. Hommez, R. Kilpper Balz, and K. H. Schleifer 1991. Characteristics of different Streptococcus suis ecovars and description of a simplified identification method. Vet. Mirobiol. 26:141-150.
5. Gottschalk, M., R. Higgins, M. Jacques, M. Beaudoin, and J. Henrichsen. 1991. Characterization of six new capsular types (23 through 28) of Streptococcus suis. J. Clin. Microbiol. 29:2590-2594.
6. Gottschalk, M., R. Higgins, M. Jacques, K. R. Mittal, and J. Henrichsen. 1989. Description of 14 new capsular types of Streptococcus suis. J. Clin. Microbiol. 27:2633-2636.
7. Gottschalk, M., S. Lacouture, and L. Odierno. 1999. Immunomagnelic isolation of Streptococcus suis serotypes 2 and 1/2 from swine tonsils. J. Clin. Microbiol. 37:2877-2881.
8. S. Higgins, R., M. Gottschalk, M. Boudreau, A. Lebrun, and J. Henrichsen. 1995. Description of six new capsular types (29-34) of Streptococcus suis J. Vet. Diagn. Investig. 7:405-406.
9. Higgins, R., M. Gottschalk, K. R. Mittal, and. M. Beaudoin. 1990. Szreptococcus suis infection in swine. A sixteen month study. Can. J. Vet. Res. 54:170-173.
10. Kebede, M., M. M. Chengappa, and J. G. Stuart. 1990. Isolation and characterization of temperature-sensitive mutants of Streptococcus suis: Efficacy trial of the mutant vaccine in mice Vet. Microbiol. 22:249-257.
11. Lamont, M. H., P. T. Edwards, and R. S. Windsor. 1980. Streptococcal meningitis in pigs: Results of a five-year survey. Vet. Rec. 107:467-469.
12. Mwaniki, C. G., I D. Robertson, and D. J. Hampson 1994. The prevalence of Streptococcus suis type 2 in Western Australian piggeries. Aust. Vet. J. 71:385-386.
13. Perch, B., E. Kjems P. Slot, and K. B. Pedersen. 1981. Biochemical and serological properties of R, S, and RS streptococci. Acta Pathol. Microbiol. Scand. B 89:167-171.
14. Perch, B., K. B. Pedersen, and J. Henrichsen. 1983. Serology of capsulated streptococci pathogenic for pigs: Six new serotypes of Streptococcus suis. J. Clin. Microbiol. 17:993-996.
15. Reams, R. Y., D. D. Harrington, L. T. Glickman, H. L. Thacker, and T. L. Bowersock. 1996. Multiple serotypes and strains of Streptococcus suis in naturally infected swine herds. J. Vet. Diagn. Investig, 8:1[9-121.
16. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
17. Sihvonen, L., D. N. Kurl, and P. Salmela. 1986. Infection with Streptococcus suis serotypes 1 and 2 in the same diseased pig. Acta Vet. Scand, 27:626-628.
18. Smith, H. E., M. Damman, J. van der Velde, F. Wagenaar, H. J. Wisselink, N. Stockhofe-Zurwieden, and M. A. Smits. 1999. Identification and characterization of the cps locus of Streptococcus suis serotype 2: The capsule protects against phagocytosis and is an important virulence factor. Infect. Immun. 67:1750-1756.
19. Smith, H. E., R. de Vries, R. van't Slot, and M. A. Smits. 2000. The cps locus of Streptococcus suis serotype 2: Genetic determinant for the synthesis of sialic acid. Microb. Pathog. 29:127-134.
20. Smith, H. E., F. H. Reek, U. Vecht, A. L. Gielkens and M. A. Smits. 1993. Repeats in an extracellular protein of weakly pathogenic strains of Streptococcus suis type 2 are absent in pathogenic strains. Infect. Immun. 61:3318-3326.
21. Smith, H. E., L. van Bruijnsvoort, H. Buijs, H. J. Wisselink, and M. A. Smits. 1999. Rapid PCR test for Streptococcus suis serotype 7. FEMS Microbiol. Lett. 178:265-270.
22. Smith, H. E., V. Veenbergen, J. van der Velde, M Damman, H. J. Wisselink, and M. A. Smits. 1999. The cps genes of Streptococcus suis serotypes 1, 2, and 9: Development of rapid serotype-specific PCR assays. J. Clin, Microbiol. 37:3146-3152.
23. Stockhofe-Zurwieden N., U. Vecht, H. J. Wisselink, H. van Lieshout, and H. E. Smith. 1996. Comparative studies on the pathogenicity of different Streptococcus suis serotype 1 strains, p. 299. In P. G. Monetti and G. Vignola (ed.), Proceedings of the 14th International Pig Veterinary Society Congress. University of Bologna, Bologna, Italy.
24. , Swildens, B., H. J. Wisselink, H. E. Smith, and J. H. M. Verheijden. 2000. Tonsillar swabs can be used in a PCR assay for the detection of virulent Streptococcus suis serotype 2 strains in weaned piglets, p. 535. In C. Cargill and S. McOrist (ed.), Proceedings of the 16th International Pig Veterinary Society Congress, Melbourne, Australia.
25. Van Leengoed, L. A., U. Vecht, and E. R. M. Verheyen. 1987. Streptococcus suis type 2 infections in pigs in the Netherlands (part two). Vet. Q. 9:111-117.
26. Vecht, U., L. A. van Leengoed, and E. R. M. Verheijen. 1985. Streptococcus suis infections in pigs in the Netherlands (part one). Vet. Q. 7:315-321.
27. Vecht, U., H. J. Wisselink, J. E. van Dijk, and H. E. Smith. 1992. Virulence of Streptococcus suis type 2 strains in newborn germfree pigs depends on phenotype. Infect. Immun. 60:550-556.
28. Wisselink, H. J. F. H. Reek, U. Vecht, N. Zurwieden, M. A. Smits, and H. E. Smith. 1999. Detection of virulent strains of Streptococcus suis type 2 and highly virulent strains of Streptococcus suis type 1 in tonsillar specimens from pigs by PCR. Vet. Microbiol. 67:143-157.
29. Wisselink, H. J., H. E. Smith, N. Stockhofe-Zurwieden, K. Peperkamp, and U. Vecht. 2000. Distribution of capsular types and production of murami-dase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries. Vet. Microbiol. 74:237-248.