A new molecular biology approach in morphology: Basic method and application of laser microdissection

Medical Electron Microscopy

Volumn 37, Issue 2, 2004, Pages 82-88

  Tachikawa, Tetsuhiko ^a   Irié, Tarou ^a  

^a SHOWA UNIVERSITY   (Japan)

Author keywords

cDNA; Invasion; Metastasis; Microarray; Microdissection; RT PCR; Squamous cell carcinoma

Indexed keywords

COMPLEMENTARY DNA;

CANCER DIAGNOSIS; CELL STRUCTURE; DIAGNOSTIC ACCURACY; DNA EXTRACTION; GENE EXPRESSION; GENE FUNCTION; GENETIC ANALYSIS; HUMAN; LASER MICRODISSECTION; MOLECULAR BIOLOGY; PRIORITY JOURNAL; PROTEIN FUNCTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; RNA EXTRACTION; SQUAMOUS CELL CARCINOMA; DIAGNOSTIC PROCEDURE; DNA MICROARRAY; GENE EXPRESSION PROFILING; LASER; METHODOLOGY; MICROMANIPULATION;

GENE EXPRESSION PROFILING; HUMANS; LASERS; MICROMANIPULATION; MOLECULAR DIAGNOSTIC TECHNIQUES; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS;

EID: 3142704586     PISSN: 09184287     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00795-003-0238-8     Document Type: Review

References (12)

1. Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhung Z, Goldstein SR, Weiss RA, Liotta LA (1996) Laser capture microdissection. Science 274:998-1001
2. Kuska B (1996) New aim-and-shoot technique speeds up cell analysis. JNCI 88:1708-1709
3. Bonner RF, Emmert-Buck MR, Cole K, Pohida T, Chuaqui C Goldstein S, Liotta LA (1997) Laser capture microdissection: molecular analysis of tissue. Science 278:1481-1483
4. Schutze K, Lahr G (1998) Identification of expressed genes by laser-mediated manipulation of single cells. Nat Biotech 16:737-742
5. Maitra A, Wistuba II, Virman AK, Sakaguchi M, Park I, Stucky A, Milchgrub S, Gibbons D, Minna JD, Gazdar AF (1999) Enrichment of epithelial cells for molecular studies. Nat Med 5:459-463
6. Jin L, Thompson CA, Qian X, Kuercker SJ, Kulig E, Lloyd RV (1999) Analysis of anterior pituitary hormone mRNA expression in immunophenotypically characterized single cells after laser capture microdissection. Lab Invest 79:511-512
7. Luo L, Salunga RC, Guo H, Bittner A, Joy KC, Galinda HE, Xiao H, Roger KE, Wan JS, Jackson MR, Erlander MG (1999) Gene expression profiles of laser-captured adjacent neuronal subtypes. Nat Med 5:117-122
8. Sgroi DC, Teng S, Robinson G, LeVangie R, Hudson HR, Elkahlun AG (1999) In vitro gene expression profile analysis of human breast cancer progression. Cancer Res 59:5656-5661
9. Irie T, Tsuchiya R, Yamamoto G, Maeda Y, Tetsuhiko T (2001) Expression analysis of mRNA in oral squamous cell carcinoma using laser microdissection and semiquantiative RT-PCR. J Sowa Univ Dent Soc 21:75-81
10. Nishino Y, Irie T, Takashi M, Hosono T, Huh N, Tachikawa T, Kuroki T (2001) PKC θII, a new isoform of PKC specifically expressed in testis. J Biol Chem 276:36711-36717
11. Irie T, Aida T, Nagoshi Y, Tsuchiya R, Ymamamoto G, Maeda Y, Saito M, Tachikawa T (2003) Laser pressure cell transfer method, a new microdissection technique to frozen section. Oral Med Pathol 8: (in press)
12. Clement-Sengewald A, Buchholz T, Schutz K (2000) Laser microdissection as new approach to prefertilization genetic diagnosis. Pathobiology 68:232-236