Usage of nonporous polymeric particles for reversed-phase high-performance liquid chromatography of oxidized and deamidated forms of recombinant human growth hormone

Journal of Chromatographic Science

Volumn 42, Issue 6, 2004, Pages 285-287

  Karlsson, Göran ^a  

^a Octapharma AB   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

POLYMER; PROPANOL; RECOMBINANT GROWTH FACTOR; SILICON DIOXIDE;

ARTICLE; DEAMINATION; DRUG DETERMINATION; DRUG OXIDATION; HORMONE DETERMINATION; POROSITY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE;

EID: 3142672858     PISSN: 00219665     EISSN: None     Source Type: Journal    
DOI: 10.1093/chromsci/42.6.285     Document Type: Article

References (11)

1. T.A. Bewley and C.H. Li. The chemistry of human pituitary growth hormone. Adv. Enzymol. 42: 73-166 (1975).
2. G.W. Becker, P.M. Tackitt, W.W. Bromer, D.S. Lefeber, and R.M. Riggin. Isolation and characterization of a sulfoxide and a desamido derivative of biosynthetic human growth hormone. Biotech. Appl. Biochem. 10: 326-37 (1988).
3. G. Teshima and E. Canova-Davis. Separation of oxidized human growth hormone variants by reversed-phase high-performance liquid chromatography: Effect of mobile phase pH and organic modifier. J. Chromatogr. 625: 207-15 (1992).
4. P. Gellerfors, B. Pavlu, K. Axelsson, C. Nyhlen, and S. Johansson. Separation and identification of growth hormone variants with high performance liquid chromatography techniques. Acta Paediatr. Scand. 370(suppl.): 93-100 (1990).
5. G.W. Becker, R.R. Bowsher, W.C. Mackellar, M.L. Poor, P.M. Tackitt, and R.M. Riggin. Chemical, physical, and biological characterization of a dimeric form of biosynthetic human growth hormone. Biotech. Appl. Biochem. 9: 478-87 (1987).
6. C. Andersson, P.O. Edlund, P. Gellerfors, Y. Hansson, E. Holmberg, C. Hult, S. Johansson, J. Kördel, R. Lundin, J. Mendel-Hartvig, B. Norén, T. Wehler, G. Widmalm, and J. Öhman. Isolation and characterization of a trisulfide variant of recombinant human growth hormone formed during expression in Escherichia coli. Int. J. Peptide Protein Res. 47: 311-21 (1996).
7. R.M. Riggin, G.K. Dorulla, and D.J. Miner. A reversed-phase high-performance liquid chromatographic method for characterization of biosynthetic human growth hormone. Anal. Biochem. 167: 199-209 (1987).
8. G. Karlsson, P. Gellerfors, A. Persson, B. Norén, P.O. Edlund, C. Sandberg, and S. Birnbaum. Separation of oxidized and deamidated human growth hormone variants by isocratic reversed-phase high-performance liquid chromatography. J. Chromatogr. A 855: 147-55 (1999).
9. T. Tojo, C. Yomota, S. Okada, and J. Kawamura. Analysis of the impurities in human growth hormone by reversed-phase high-performance liquid chromatography. Iyakuhin Kenkyu. 27: 815-20 (1996).
10. D.R. Jenke. Practical examination of a nonporous silica stationary phase for reversed-phase fast LC applications. J. Chromatogr. Sci. 34: 362-67 (1996).
11. K. Kalghatgi and C. Horváth. Rapid analysis of proteins and peptides by reversed-phase chromatography. J. Chromatogr. 398: 335-39 (1987).