Isolation and characterization of a trisulfide variant of recombinant human growth hormone formed during expression in Escherichia coli

International Journal of Peptide and Protein Research

Volumn 47, Issue 4, 1996, Pages 311-321

  Andersson, Claes ^a   Edlund, Per Olof ^a   Gellerfors, Pär ^a   Hansson, Yngve ^a   Holmberg, Erland ^a   Hult, Carina ^a   Johansson, Stig ^a   Kordel, Johan ^a   Lundin, Ronny ^a   Mendel Hartvig, I B ^a   Norén, Bengt ^a   Wehler, Thomas ^a   Widmalm, Göran ^a   Öhman, Johan ^a  

^a PFIZER INC   (United States)

Author keywords

Biosensor; Circular dichroism; hGH; Mass spectrometry; Nuclear magnetic resonance spectroscopy; Recombinant protein; Synthetic peptide; Thiocystine; Trisulfide; Tryptic peptides

Indexed keywords

HUMAN GROWTH HORMONE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHROMATOGRAPHY; CIRCULAR DICHROISM; DRUG MANUFACTURE; DRUG RECEPTOR BINDING; ESCHERICHIA COLI; GENE EXPRESSION; HUMAN; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN VARIANT; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ESCHERICHIA COLI;

EID: 0029990937     PISSN: 03678377     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1399-3011.1996.tb01360.x     Document Type: Article

References (31)

1. Bewely, T. & Li, C.H. (1975) Adv. Enzymol. 42,73-166
2. Abdel-Meguid, S.S., Shieh, H.S., Smith, W.W., Dayringer, H.H., Viol &, B.N. & Bentle, L.A. (1987) Froc. Natl. Acad. Sei. 84,6434-6437
3. deVos, A.M., Ultsch, M. & KossiakofT, A.A. (1992) Science 255,306-312
4. Cunningham, B.C. & Wells, J.A. (1993) J. Mol. Biol. 234,554-563
5. Moore, D.D., Walker, M.D., Diamond, D.J., Conkling, A. & Goodman, H.M. (1982) Recent Prog. Horm. Res. 38,197-225
6. Wallis, M. (1980) Nature (London) 284,512
7. De Netto, P.M., Moore, D.D., Goodman, P.L., Seeburg, P. & Hamer, D.H. (1981) Proc. Natl. Acad. Sei. 78,7398-7402
8. Gellerfors, P., Pavlu, B., Axelsson, K., Nyhlén, C. & Johansson, S. (1990) Ada Paediatr. Scand. 370,93-100
9. Houghten, R.A., Glaser, C.B. & Li, C.H. (1977) Arch. Biochem. Biophys. 178,350-355
10. Pavlu, B. & Gellerfors, P. (1993) Bioseparation 3,257-265
11. Jespersen, A. M., Christensen, T. Klausen, N. K., Nielsen, P.P. & Sorensen, H. H. (1994) Eur. J. Biochem. 219,365-373
12. Lundin, R.H.L., Norén, B.E. & Edlund, P.O. ( 1994) Tetrahcdr. Lett. 35,6339-6342
13. Hunkapiller M.W. (1988) in Macromolecular Sequencing & Synthesis. Selected Methods & Applications (Schlesinger, D.H., ed), pp. 45-58, Alan R. Liss Inc., New York
14. Piantini, U., Sorensen, O.W. & Ernst, R. R. (1982) J. Am. Cliem. Soc. 104,6800-6801
15. Braunschweiler, L. & Ernst, R.R. (1983) J. Magn. Reson. 53,521-528
16. Jeneer, J., Meier, B.H., Bachmann, P. & Ernst, R.R. (1979) J. Chem. Phys. 71,4546-4553
17. Bax, A. & Davies, D.G. (1985) J. Magn. Reson. 63,207-213
18. Marion, D., Ikura, M. & Bax, A. (1989) J. Magn. Reson. 84,425-430
19. BIAcore System Manual, Pharmacia Biosensor AB
20. Stenberg, E., Roos, H. & Urbaniczky, C. (1991) J. Colloid Interface Sei. 143,513-526
21. Briggs, R.G. & Fee, J.A. (1978) Bloc/lint. Biophys. Acta 537,100-109
22. Wuthrich, K. (1986) NMR of Proteins and Nucleic Acids, Wiley Interscience, New York
23. Mott, A.W., Slomczynska, U. & Barany, G. (1984) Forum Peptides Le Cap d'Agde,pp. 321-324
24. Abdolrasulnia, R. & Wood, J.L. (1980) Bioorg. Chem. 9,253-260
25. Jonsson, S. & Raa, J. (1980) J. Food Sei. 45,1641-1644
26. Rivett, D.E., Roxburgh, CM. & Savage, W.E. (1965) Proceedings of the International Wool Textiles Research Conference, CSIRO, Melbourn,pp. 419-428
27. Ghadimi, M. & Hill, R.R. (1991) J. Chem. Soc., Chem. Commun.,903-904
28. Szczepkowski, T.W. & Wood, J.L. (1967) Biochim. Biophys. Acta 139,469-478
29. Fuh, G., Colosi, P., Wood, W.I. & Wells, J.A. (1993) J. Biol. Chem. 268,5376-5381
30. Wells J.A. & deVos A. (1993) Anna. Rer. Biophys. Biomol. Struct. 22,329-351
31. Thomsen, M.K., Hansen, B.S., Nilsson, P., Nowak, J., Johansen, P.B., Thomsen, P.D. & Christiansen, J. (1994) Pharmacol. Toxicol. 74,351-358