Enrichment and rapid detection of Bacillus anthracis spores by direct immunofluorescence assay

Indian Journal of Microbiology

Volumn 45, Issue 4, 2005, Pages 269-271

  Goel, A K ^a   Kamboj, D V ^a   Singh, L ^a  

^a DEFENCE RESEARCH AND DEVELOPMENT ESTABLISHMENT   (India)

Author keywords

Anthrax; Aqueous polymer bi phasic buffer; B. anthracis; Immunofluorescence; Polyclonal antibodies

Indexed keywords

BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS);

EID: 31144455535     PISSN: 00468991     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Dragon DC & Rennie RP (2001) Evaluation of spore extraction and purification methods for selective recovery of viable Bacillus anthracis spores. Lett Appl Microbiol 33:100-105.
2. Dragon DC, Rennie RP & Elkin BT (2001) Detection of anthrax spores in endemic regions of northern Canada. J Appl Microbiol 91:435-441.
3. Cheun HI, Makino SI, Watarai M, Erdenebaatar J, Kawamoto K & Uchida I (2003) Rapid and effective detection of anthrax spores in soil by PCR. J Appl Microbiol 95:728-733.
4. Stewart BT & Halvorson HO (1953) Studies on the spores of aerobic bacteria. J Bacteriol 65:160-166.
5. Harlow E & Lane D (1988) Antibodies: a laboratory manual. Cold Spring Harbour Press, New York, 355 p.
6. Hasan JAK, Huq A, Tamplin ML, Siebeling RJ & Colwell RR (1994) A novel kit for rapid detection of Vibrio cholerae O1. J Clin Microbiol 32:249-252.
7. Johnson GD, Davison RS, Mcnamee KC, Russed G, Goodwin D & Holborow EJ (1982) Fading of immunofluorescence during microscope: a study of the phenomenon and its remedy. J Immunol Methods 54:231-242.
8. Philips AP, Martin KL & Broster MG (1983) Differentiation between spores of Bacillus anthracis and Bacillus cereus by quantitative immunofluorescence technique. J Clin Microbiol 17:41-47.
9. Doyle RJ, Nedjat-Haiem F & Singh JS (1984) Hydrophobic characteristics of Bacillus spores. Curr Microbiol 10:329-332.
10. Wiencek KM, Klapes NA & Foegeding PM (1990) Hydrophobicity of Bacillus and Clostridium spores. Appl Environ Microbiol 56:2600-2605
11. Sacks LE & Alderson G (1961) Behavior of bacterial spores in aqueous polymer two-phase systems. J Bacteriol 82:331-341
12. Agarwal GS, Kamboj DV, Alam SI, Dixit M & Singh L (2002) Environmental detection of Bacillus anthracis spores. Curr Sci 83:697-69