Sodium dodecyl sulfate agarose gel electropheresis and electroelution of high molecular weight human salivary mucin

Clinical Oral Investigations

Volumn 9, Issue 4, 2005, Pages 76-78

  Gabriel, Matthias ^a   Zentner, Andrej ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Agarose; Electroelution; Electrophoresis; Mucin; Saliva

Indexed keywords

GLYCOPROTEIN; MUCIN; SALIVA PROTEIN;

AGAR GEL ELECTROPHORESIS; ARTICLE; FILTRATION; HUMAN; ISOELECTRIC FOCUSING; ISOLATION AND PURIFICATION; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ELECTROPHORESIS, AGAR GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FILTRATION; GLYCOPROTEINS; HUMANS; ISOELECTRIC FOCUSING; MOLECULAR WEIGHT; MUCINS; SALIVARY PROTEINS;

EID: 31044454268     PISSN: 14326981     EISSN: 14363771     Source Type: Journal    
DOI: 10.1007/s00784-005-0007-2     Document Type: Article

References (14)

1. Cao X, Bansil R, Bhaskar KR, Turner BS, LaMont JT, Niu N, Afdhal NH (1995) pH-Dependent conformational change of gastric mucin leads to sol-gel transition. Biophys J 76:1250-1258
2. Carlstedt I, Lindgren H, Sheehan JK, Ulmsten U, Wingerup L (1983) Isolation and characterization of human cervical-mucus glycoproteins. Biochem J 211:13-22 6409086
3. Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W (1991) Current protocols in immunology, vol. II, suppl. 46, chapters 8.9.1 and 8.10.1 Wiley, New York
4. Fischer BE, Thomas KB, Schlokat U, Dorner F (1998) Triplet structure of human von Willebrand factor. Biochem J 331:483-488 9531488
5. Green DRJ, Embery G (1985) Structural studies on a sulphated glycoprotein preparation isolated from human saliva. Arch Oral Biol 30:859-861 10.1016/0003-9969(85)90144-X
6. Krizek DR, Rick ME (2000) A rapid method to visualize von Willebrand factor multimers by using agarose gel electrophoresis, immunolocalization and luminographic detection. Thromb Res 97:457-462 10.1016/S0049-3848(99)00196-6
7. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685 10.1038/227680a0
8. Loomis RE, Prakobphol A, Levine MJ, Reddy MS, Jones PC (1987) Biochemical and biophysical comparison of two mucins from human submandibular-sublingual saliva. Arch Biochem Biophys 258:452-464 10.1016/0003-9861(87)90366-3
9. Lottspeich F, Zorbas H (1998) Bioanalytik. Spektrum Verlag, Heidelberg, pp 225
10. Paszkiewicz-Gadek A, Gindzinski A, Porowska H (1995) The use of preparative polyacrylamide gel electrophoresis and electroelution for purification of mucus glycoproteins. Anal Biochem 226:263-267 10.1006/ abio.1995.1224
11. Preobrazhensky A (1993) Electrophoresis of neurochordins, a family of high molecular weight neural tissue glycoproteins, on horizontal submerged agarose gels in the presence of dodecyl sulfate. Anal Biochem 209:315-317 10.1006/abio.1993.1125
12. Tabak LA (1995) In defense of the oral cavity: Structure, biosynthesis, and function of salivary mucins. Annu Rev Physiol 57:547-564 10.1146/ annurev.ph.57.030195.002555
13. Thornton DJ, Howard M, Devine PL, Sheehan JK (1995) Methods for separation and deglycosylation of mucin subunits. Anal Biochem 227:162-167 10.1006/abio.1995.1266
14. Veerman ECI, Valentijn-Benz M, Bank RA, Nieuw Amerongen AV (1989) Isolation of high molecular weight mucins from human whole saliva by ultracentrifugation. J Biol Buccale 17:307-312 2613704