A rapid method to visualize von Willebrand factor multimers by using agarose gel electrophoresis, immunolocalization and luminographic detection

Thrombosis Research

Volumn 97, Issue 6, 2000, Pages 457-462

  Krizek, Dennis R ^a   Rick, Margaret E ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Method; Multimers; von Willebrand disease classification; von Willebrand factor

Indexed keywords

PROTEIN SUBUNIT; VON WILLEBRAND FACTOR;

AGAR GEL ELECTROPHORESIS; ARTICLE; CONTROLLED STUDY; HUMAN; MOLECULAR WEIGHT; PLASMA; PRIORITY JOURNAL; PROTEIN DETERMINATION; THROMBOCYTE;

BLOOD PLATELETS; DENSITOMETRY; ELECTROPHORESIS, AGAR GEL; HUMANS; IMMUNOBLOTTING; LUMINESCENT MEASUREMENTS; VON WILLEBRAND FACTOR;

EID: 0033994186     PISSN: 00493848     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0049-3848(99)00196-6     Document Type: Article

References (20)

1. Titani K., Kumart S., Takio K., Ericsson L.H., Wade R.D., Ashida K., Walsh K.A., Chopek M.W., Sadler J.E., Fujikawa K. Amino acid sequence of human von willebrand factor. Biochem. 25:1986;3171-3184.
2. Hoyer L., Shainoff J. Factor VIII-related protein circulates in normal human plasma as high molecular weight multimers. Blood. 55:1980;1056-1059.
3. Wagner D. Cell biology of von Willebrand factor. Annu Rev Cell Biol. 6:1990;217-246.
4. Jaffe E., Hoyer L., Nachman R. Synthesis of antihemophilic factor antigen by cultured endothelial cells. J Clin Invest. 52:1975;2757-2764.
5. Sporn L., Chavin S., Marder V. Biosynthesis of von Willebrand protein by human megakaryocytes. J Clin Invest. 76:1985;1102-1106.
6. Weiss H. von Willebrand factor and platelet function. Ann NY Acad Sci. 614:1991;125-137.
7. Brinkhous K., Sandberg H., Garris J., Mattsson C., Palm M., Griggs T., Read M.S. Purified human factor VIII procoagulant protein. Comparative hemostatic response after infusion into hemophilic and von Willebrand disease dogs Proc Natl Acad Sci USA. 82:1985;8752-8755.
8. Sadler J. A revised classification of von Willebrand disease. Thromb Haemost. 71:1994;520-525.
9. Nichols W., Ginsburg D. von Willebrand disease. Medicine. 76:1997;1-20.
10. Furlong B., Peake I. An electroblotting technique for the detection of factor VIII/von Willebrand factor multimers in plasma. Br J Haematol. 53:1983;641-653.
11. Bukh A., Ingerslev J., Stenbjerg S., Hundahl Møller N.P. The multimeric structure of plasma f VIII:Rag studied by electroelution and immunoperoxidase detection. Thromb Res. 43:1986;579-584.
12. Brosstad F., Kjonniksen I., Ronning B., Stormorken H. Visualization of von Willebrand factor multimers by enzyme-conjugated secondary antibodies. Thromb Haemost. 55:1986;276-278.
13. Dalton R.G., Lasham A., Savidge G.F. A new rapid semi-dry blotting technique for multimeric sizing of von Willebrand factor. Thromb Res. 50:1988;345-349.
14. Farias C., Kempfer A., Blanco A., Woods A., Lazzari M.A. Visualization of the multimeric structure of von Willebrand factor by immunoenzymatic stain using avidin-peroxidase complex instead of avidin-biotin peroxidase complex. Thromb Res. 53:1989;513-518.
15. Budde U., Schneppenheim R., Plendl H., Dent J., Ruggeri Z.M., Zimmerman T.S. Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes. Thromb Haemostas. 63:1990;312-315.
16. Raines G., Aumann H., Sykes S., Street A. Multimeric analysis of von Willebrand factor by molecular sieving electrophoresis in sodium dodecyl sulphate agarose gel. Thromb Res. 60:1990;201-212.
17. Schneppenheim R., Budde U., Dahlmann N., Rautenberg P. Luminography - A new, highly sensitive visualization method for electrophoresis. Electrophoresis. 12:1991;367-372.
18. Baillod P., Affolter G., Kurt G.H., Pflugshaupt R. Multimeric analysis of von Willebrand factor by vertical sodium dodecyl sulphate agarose gel electrophoresis, vacuum blotting technology and sensitive visualization by alkaline phosphatase anti-alkaline phosphatase complex. Thromb Res. 66:1992;745-755.
19. Metzner H., Hermentin P., Cuesta-Linker T., Langner S., Muller H.G., Friedebold J. Characterization of factor VIII/von Willebrand factor concentrates using a modified method of von Willebrand factor multimer analysis. Haemophilia. 4(Suppl):1998;25-32.
20. Gralnick H.R., Williams S.B., McKeown L.P., Krizek D.M., Shafer B.C., Rick M.E. Platelet von Willebrand factor. Comparison with plasma von Willebrand factor Thromb Res. 38:1985;623-633.