Subtractive cloning of cDNA from Aspergillus oryzae differentially regulated between solid-state culture and liquid (submerged) culture

Current Genetics

Volumn 41, Issue 4, 2002, Pages 275-281

  Akao, Takeshi ^a   Gomi, Katsuya ^b   Goto, Kuniyasu ^a   Okazaki, Naoto ^a   Akita, Osamu ^a  

^a NATIONAL RESEARCH INSTITUTE OF BREWING   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

Author keywords

Aspergillus oryzae; cDNA subtraction; Differential gene expression; Solid state culture

Indexed keywords

CARRIER PROTEIN; CELL ENZYME; COMPLEMENTARY DNA; FUNGAL ENZYME; GENE PRODUCT;

ARTICLE; ASEXUAL REPRODUCTION; ASPERGILLUS ORYZAE; CELL DIFFERENTIATION; CONTROLLED STUDY; CULTURE MEDIUM; DNA LIBRARY; DOWN REGULATION; ENZYME RELEASE; ENZYME SYNTHESIS; FUNGUS HYPHAE; GENE CONTROL; GENE EXPRESSION; GENE FUNCTION; GENE IDENTIFICATION; GENE ISOLATION; GENE SEQUENCE; GENETIC CODE; LIQUID CULTURE; MOLECULAR CLONING; MOLECULAR HYBRIDIZATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PREDICTION; PRIORITY JOURNAL; REGULATORY MECHANISM; SEQUENCE ANALYSIS; SOLID STATE; UPREGULATION;

ASPERGILLUS ORYZAE; CLONING, MOLECULAR; CULTURE MEDIA; DNA, COMPLEMENTARY; EXPRESSED SEQUENCE TAGS; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL;

ASPERGILLUS; ASPERGILLUS ORYZAE; FUNGI; MYXOGASTRIA;

EID: 12244299847     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-002-0314-y     Document Type: Article

References (16)

1. Altschul SF, Madden TL, Alejandro A, Schaffer, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389-3402
2. Cathala G, Savouret J-F, Mendez B, West BL, Karin M, Martial JA, Baxter JD (1983) A method for isolation of intact, translationally active ribonucleic acid. DNA 2:329-335
3. Chomczynski P (1993) A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15:532-537
4. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159
5. Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991-1995
6. Diatchenko L, Lukyanov S, Lau Y-FC, Siebert PD (1999) Suppression subtractive hybridization: a versatile method for identifying differentially expressed genes. Methods Enzymol 303:349-380
7. Gervais P, Bensoussan M (1994) Solid-state fermentations of the genus Aspergillus. In: Smith JE (ed) Aspergillus. Plenum Press, New York, pp 101-140
8. Hata Y, Ishida H, Kojima Y, Ichikawa E, Kawato A, Suginami K, Imayasu S (1997) Comparison of two glucoamylases produced by Aspergillus oryzae in solid-state culture (koji) and submerged culture. J Ferment Bioeng 84:532-537
9. Hata Y, Ishida H, Ichikawa E, Kawato A, Suginami K, Imayasu S (1998) Nucleotide sequence of an alternative glucoamylase-encoding gene (glaB) expressed in solid-state culture of Aspergillus oryzae. Gene 207:127-134
10. Ishida H, Hata Y, Kojima Y, Ichikawa E, Kawato A, Suginami K, Imayasu S (1998) Regulation of the glucoamylase-encoding gene (glaB), expressed in solid-state culture (koji) of Aspergillus oryzae. J Ferment Bioeng 86:301-307
11. Ishida H, Hata Y, Kawato A, Abe Y, Suginami K, Imayasu S (2000) Identification of functional elements that regulate the glucoamylase-encoding gene (glaB) expressed in solid-state culture of Aspergillus oryzae. Curr Genet 37:373-379
12. Lee DW, Koh JS, Kim JH, Chae K-S (1999) Cloning and nucleotide sequence of one of the most highly expressed genes, a pdcA homologue of Aspergillus nidulans, in Aspergillus oryzae. Biotechnol Lett 21:139-142
13. Sagerstrom CG, Sun BI, Sive HL (1997) Subtractive cloning: past, present, and future. Annu Rev Biochem 66:751-783
14. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
15. Sato K, Sudo S (1999) Small-scale solid-state fermentations. In: Demain AL, Davis JE (eds) Manual of industrial microbiology and biotechnology, 2nd edn. AMS Press, Washington, D.C., pp 61-79
16. Yamane Y, Yoshi M, Mikami S, Fukuda H, Kizaki Y (2000) A solid-state culture system using a cellulose carrier containing defined medium as a useful tool for investigating characteristics of koji culture. J Biosci Bioeng 89:33-39