Improved clonality analysis of multifocal bladder tumors by combination of histopathologic organ mapping, loss of heterozygosity, fluorescence in situ hybridization, and p53 analyses

Human Pathology

Volumn 37, Issue 2, 2006, Pages 143-151

  Denzinger, Stefan ^a   Mohren, Kristin ^b   Knuechel, Ruth ^c   Wild, Peter J ^d   Burger, Maximilian ^a   Wieland, Wolf F ^a   Hartmann, Arndt ^a   Stoehr, Robert ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b Institute of Pathology and Cytology   (Germany)

^c UNIVERSITY HOSPITAL RWTH AACHEN   (Germany)

^d UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

Author keywords

Clonality; Fluorescence in situ hybridization; LOH analysis; Multifocal bladder cancer; Organ mapping

Indexed keywords

PROTEIN P53;

ADULT; ARTICLE; BLADDER TUMOR; CHROMOSOME 17P; CHROMOSOME 9; CHROMOSOME ABERRATION; CHROMOSOME MICRODISSECTION; CLONAL VARIATION; CONTROLLED STUDY; CYSTECTOMY; EXON; FEMALE; FLUORESCENCE IN SITU HYBRIDIZATION; GENE MUTATION; GENE SEQUENCE; GENOME ANALYSIS; HETEROZYGOSITY LOSS; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; LEIOMYOSARCOMA; MALE; MULTIPLE CANCER; VAGINA CANCER;

CHROMOSOMES, HUMAN, PAIR 17; CHROMOSOMES, HUMAN, PAIR 9; CLONE CELLS; DNA MUTATIONAL ANALYSIS; FEMALE; GENES, P53; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; LOSS OF HETEROZYGOSITY; MALE; MICROSATELLITE REPEATS; MIDDLE AGED; URINARY BLADDER NEOPLASMS;

EID: 30744477700     PISSN: 00468177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.humpath.2005.10.014     Document Type: Article

References (33)

1. R.J. Black, F. Bray, J. Ferloy, and D.M. Parkin Cancer incidence and mortality in the European Union Cancer registry data and estimates of national incidence for 1990 Eur J Cancer 33 1997 1075 1107
2. M. Knowles What we could do now: molecular pathology of bladder cancer J Clin Pathol: Mol Pathol 54 2001 215 221
3. B.J. Duggan, S.B. Gray, J.J. McKnight, C.J. Watson, S.R. Johnston, and K.E. Williamson Oligoclonality in bladder cancer: the implication for molecular therapies J Urol 171 2004 419 424
4. R. Dahse, D. Gartner, W. Werner, J. Schubert, and K. Junker p53 mutations as an identification marker for the clonal origin of bladder tumors and its recurrences Oncol Rep 10 2003 2033 2037
5. C. Hafner, R. Knuechel, R. Stoehr, and A. Hartmann Clonality of multifocal urothelial carcinomas: 10 years of molecular genetic studies Int J Cancer 101 2002 1 6
6. R. Stoehr, A. Hartmann, E. Hiendlymeyer, K. Muerle, W. Wieland, and R. Knuechel Oligoclonality of early lesions of the urothelium as determined by microdissection-supported genetic analysis Pathobiology 68 2000 165 172
7. I.P.M. Tomlinson, M.B.K. Lambros, and R.R. Roylance Loss of heterozygosity analysis: practically and conceptually flawed? Genes Chromosomes Cancer 34 2002 349 353
8. C.W. Kan, C.P. Fredlake, E.A. Doherty, and A.E. Barron DNA sequencing and genotyping in miniaturized electrophoresis systems Electrophoresis 25 2004 3564 3588
9. K.K. Jain Current status of fluorescent in-situ hybridisation Med Device Technol 15 2004 14 17
10. R. Stoehr, R. Knuechel, and J. Boecker Histologic-genetic mapping by allele-specific PCR reveals intraurothelial spread of p53 mutant tumor clones Lab Invest 82 2002 1553 1561
11. L.H. Sobin, and C. Wittekind TNM classification of malignant tumors 6th ed. 2002 Wiley-Liss Weinheim
12. J.N. Eble, G. Sauter, I.J. Epstein, and I.A. Sesterhenn WHO classification of tumors: tumors of the urinary system and male genital organs 2004 Springer Berlin
13. R. Stoehr, P. Wild, and A. Hartmann Lasermicrodissection-an important prerequisite for the molecular-genetic analysis of bladder cancer Pathol Res Pract 199 2003 355 362
14. A. Hartmann, G. Schlake, and D. Zaak Occurrence of chromosome 9 and p53 alterations in multifocal dysplasia and carcinoma in situ of human urinary bladder Cancer Res 62 2002 809 818
15. A. Hartmann, U. Rosner, and G. Schlake Clonality and genetic divergence in multifocal low-grade superficial urothelial carcinomas as determined by chromosome 9 and p53 deletion analysis Lab Invest 80 2000 709 718
16. J. Schlegel, T. Bocker, H. Zirngibl, F. Hofstadter, and J. Ruschoff Detection of microsatellite instability in human colorectal carcinomas using a non-radioactive PCR-based screening technique Virchows Arch 426 1995 223 227
17. R. Stoehr, C. Wissmann, and H. Suzuki Deletions of chromosome 8p and loss of sFRP1 expression are progression markers of papillary bladder cancer Lab Invest 84 2004 465 478
18. J.L. Petzoldt, I.M. Leigh, P.G. Duffy, C. Sexton, and J.R. Masters Immortalisation of human urothelial cells Urol Res 23 1995 377 380
19. N.S. Sheperd, B.D. Pfrogner, and J.N. Coulby Preparation and screening of an arrayed human genomic library generated with the P1 cloning system Proc Natl Acad Sci U S A 91 1994 2629 2633
20. S. Lee, and S. Rasheed A simple procedure for maximum yield of high-quality plasmid DNA Biotechniques 9 1990 676 679
21. A. Hartmann, H. Blaszyk, and R.M. McGovern p53 gene mutations inside and outside exons 5-8: the patterns differ in breast and other cancers Oncogene 10 1995 681 688
22. A.J. Levine p53, the cellular gatekeeper for growth and division Cell 88 1997 323 331
23. C. Hafner, R. Knuechel, and L. Zanardo Evidence for oligoclonality and tumor spread by intraluminal seeding in multifocal urothelial carcinomas Oncogene 20 2001 4910 4915
24. E. Baud, P. Catilina, J.P. Boiteux, and Y.J. Bignon Human bladder cancers and normal bladder mucosa present the same hot spot of heterozygous chromosome-9 deletion Int J Cancer 77 1998 821 824
25. R. Stoehr, S. Zietz, and M. Burger Deletions of chromosomes 9 and 8p in histologically normal urothelium of patients with bladder cancer Eur Urol 47 2005 58 63
26. L.B. Czerniak, V. Chatruvedi, J.Y. Ro, D.A. Johnston, S. Hodges, and W.F. Benedict Genetic modeling of human urinary bladder carcinogenesis Genes Chromosomes Cancer 27 2000 392 402
27. A.M. Cianciully, C. Leonardo, and F. Guadagni Genetic instability in superficial bladder cancer and adjacent mucosa: an interphase cytogenetics study Hum Pathol 34 2003 212 214
28. K. Junker, D. Boerner, W. Schulze, M. Utting, J. Schubert, and W. Werner Analysis of genetic alterations in normal bladder urothelium Urology 62 2003 1134 1138
29. E.C. Obermann, K. Junker, and R. Stoehr Frequent genetic alterations in flat urothelial hyperplasias and concomitant papillary bladder cancer as detected by CGH, LOH, and FISH analyses J Pathol 199 2003 50 57
30. P. Hainaut, and M. Hollstein p53 and human cancer: the first ten thousand mutations Adv Cancer Res 77 2000 81 127
31. M. Olivier, R. Eeles, M. Hollstein, M.A. Khan, C.C. Harris, and P. Hainaut The IARC TP53 database: new online mutation analysis and recommendations to users (version R9) Hum Mutat 19 2002 607 614
32. N.D. Smith, J.N. Rubenstein, S.E. Eggener, and J.M. Kozlowski The p53 tumor suppressor gene and nuclear protein: basic science review and relevance in the management of bladder cancer J Urol 169 2003 1219 1228
33. A.G. Knudson Hereditary cancer: two hits revisited J Cancer Res Clin Oncol 1223 1996 135 140