The Aspergillus nidulans amdS gene as a marker for the identification of multicopy T-DNA integration events in Agrobacterium-mediated transformation of Aspergillus awamori

Current Genetics

Volumn 45, Issue 6, 2004, Pages 399-403

  Michielse, C B ^a   Ram, A F J ^a,b   van den Hondel, C A M J J ^a,b  

^a LEIDEN UNIVERSITY   (Netherlands)

^b TNO   (Netherlands)

Author keywords

Agrobacterium tumefaciens; amdS selection marker; Aspergillus awamori; Filamentous fungi; Multicopy T DNA integration; Transformation

Indexed keywords

ACETAMIDASE; ACETAMIDE; AGAR; DNA; FUNGAL ENZYME; HYGROMYCIN; NITROGEN; UNCLASSIFIED DRUG;

AGAR MEDIUM; ANALYTIC METHOD; ANTIBIOTIC RESISTANCE; ARTICLE; ASPERGILLUS AWAMORI; ASPERGILLUS NIDULANS; CONTROLLED STUDY; CULTURE MEDIUM; DNA INTEGRATION; FREQUENCY ANALYSIS; FUNGUS IDENTIFICATION; GENE TRANSFER; GENETIC SELECTION; GENETIC TRANSFORMATION; GROWTH RATE; INTERMETHOD COMPARISON; MARKER GENE; NONHUMAN; PRIORITY JOURNAL; RHIZOBIUM;

AMIDOHYDROLASES; ASPERGILLUS; CINNAMATES; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; GENETIC MARKERS; HYGROMYCIN B; PLASMIDS; RHIZOBIUM; TRANSFORMATION, GENETIC;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; ASPERGILLUS; ASPERGILLUS AWAMORI; EMERICELLA NIDULANS; FUNGI;

EID: 3042774702     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-004-0500-1     Document Type: Article

References (35)

1. Abuodeh RO, Orbach MJ, Mandel MA, Das A, Galgiani JN (2000) Genetic transformation of Coccidioides immitis facilitated by Agrobacterium tumefaciens. J Infect Dis 181:2106-2110
2. Amey RC, Athey-Pollard A, Burns C, Mills PR, Bailey A, Foster GD (2002) PEG-mediated and Agrobacterium-mediated transformation in the mycopathogen Verticillium fungicola. Mycol Res 106:4-11
3. Beijersbergen AG, Den Dulk A, Schilperoort RA, Hooykaas PJ (1992) Conjugative transfer by the virulence system of Agrobacterium tumefaciens. Science 256:1324-1327
4. Bundock P, Dulk-Ras A, Beijersbergen A, Hooykaas PJ (1995) Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J 14:3206-3214
5. Bundock P, Attikum H van, Dulk-Ras A, Hooykaas PJ (2002) Insertional mutagenesis in yeasts using T-DNA from Agrobacterium tumefaciens. Yeast 19:529-536
6. Combier JP, Melayah D, Raffier C, Gay G, Marmeisse R (2003) Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the symbiotic ectomycorrhizal fungus Hebeloma cylindrosporum. FEMS Microbiol Lett 220:141-148
7. Corrick CM, Twomey AP, Hynes MJ (1987) The nucleotide sequence of the amdS gene of Aspergillus nidulans and the molecular characterization of 5′ mutations. Gene 53:63-71
8. Covert SF, Kapoor P, Lee M, Briley A, Nairn CJ (2001) Agrobacterium-mediated transformation of Fusarium circinatum. Mycol Res 105:259-264
9. Degefu Y, Hanif M (2003) Agrobacterium tumefaciens-mediated transformation of Helminthosporium turcicum, the maize leaf-blight fungus. Arch Microbiol 108:279-284
10. Fitzgerald AM, Mudge AM, Gleave AP, Plummer KM (2003) Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis. Mycol Res 107:803-810
11. Gelvin SB (2000) Agrobacterium and plant genes involved in T-DNA transfer and integration. Annu Rev Plant Physiol Plant Mol Biol 51:223-256
12. Gouka RJ, Gerk C, Hooykaas PJ, Bundock P, Musters W, Verrips CT, Groot MJ de (1999) Transformation of Aspergillus awamoriby Agrobacterium tumefaciens-mediated homologous recombination. Nat Biotechnol 17:598-601
13. Groot MJ de, Bundock P, Hooykaas PJ, Beijersbergen AG (1998) Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol 16:839-842
14. Hanegraaf PP, Punt PJ, Hondel CA van den, Dekker J, Yap W, Verseveld HW van, Stouthamer AH (1991) Construction and physiological characterization of glyceraldehyde-3-phosphate dehydrogenase overproducing transformants of Aspergillus nidulans. Appl Microbiol Biotechnol 34:765-771
15. Hynes MJ, Pateman JA (1970) The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. I. Mutants able to utilize acrylamide. Mol Gen Genet 108:97-106
16. Kelly JM, Hynes MJ (1985) Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans. EMBO J 4:475-479
17. Kolar M, Punt PJ, Hondel CA van den, Schwab H (1988) Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene. Gene 62:127-134
18. Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (2001) Genetic transformation of HeLa cells by Agrobacterium. Proc Natl Acad Sci USA 98:1871-1876
19. Leclerque A, Wan H, Abschutz A, Chen S, Mitina GV, Zimmermann G, Schairer HU (2003) Agrobacterium-mediated insertional mutagenesis (AIM) of the entomopathogenic fungus Beauveria bassiana. Curr Genet (in press)
20. Malonek S, Meinhardt F (2001) Agrobacterium tumefaciens-mediated genetic transformation of the phytopathogenic ascomycete Calonectria morganii. Curr Genet 40:152-155
21. Mattanovich D, Ruker F, Machado AC, Laimer M, Regner F, Steinkellner H, Himmler G, Katinger H (1989) Efficient transformation of Agrobacterium spp. by electroporation. Nucleic Acids Res 17:6747
22. Meyer V, Mueller D, Strowig T, Stahl U (2003) Comparison of different transformation methods for Aspergillus giganteus. Curr Genet 43:371-377
23. Michielse CB, Ram AFJ, Hooykaas PJJ, Hondel CAMJJ van den (2004a) Agrobacterium-mediated transformation of Aspergillus awamori in the absence of full length VirD2, VirC2 and VirE2 leads to the insertion of aberrant T-DNA structures. J Bacteriol (in press)
24. Michielse CB, Ram AFJ, Hooykaas PJJ, Hondel CAMJJ van den (2004b) Role of bacterial virulence proteins in Agrobacterium-mediated transformation of Aspergillus awamori. Fungal Genet Biol (in press)
25. Mullins ED, Chen X, Romaine P, Raina R, Geiser DM, Kang S (2001) Agrobacterium-mediated transformation of Fusarium oxysporum: an efficient tool for insertional mutagenesis and gene transfer. Phytopathology 91:173-180
26. Pardo AG, Hanif M, Raudaskoski M, Gorfer M (2002) Genetic transformation of ectomycorrhizal fungi mediated by Agrobacterium tumefaciens. Mycol Res 106:132-137
27. Penttila M, Nevalainen H, Ratto M, Salminen E, Knowles J (1987) A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. Gene 61:155-164
28. Punt PJ, Hondel CA van den (1992) Transformation of filamentous fungi based on hygromycin B and phleomycin resistance markers. Methods Enzymol 216:447-457
29. Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, Hondel CA van den (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56:117-124
30. Rho HS, Kang S, Lee YH (2001) Agrobacterium tumefaciens-mediated transformation of the plant pathogenic fungus, Magnaporthe grisea. Mol Cells 12:407-411
31. Rodriguez RJ, Yoder OC (1987) Selectable genes for transformation of the fungal plant pathogen Glomerella cingulata f.sp. phaseoli (Colletotrichum lindemuthianum). Gene 54:73-81
32. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
33. Verdoes JC, Punt PJ, Hondel CAM van den (1995) Molecular genetic strain improvement for the overproduction of fungal proteins by filamentous fungi. Appl Microbiol Biotechnol 43:195-205
34. Vijn I, Govers F (2003) Agrobacterium tumefaciens mediated transformation of the oomycete plant pathogen Phytophthora infestans. Mol Plant Pathol 4:459-467
35. Zupan JR, Zambryski P (1995) Transfer of T-DNA from Agrobacterium to the plant cell. Plant Physiol 107:1041-1047