Cytotoxicity and proliferation studies with arsenic in established human cell lines: Keratinocytes, melanocytes, dendritic cells, dermal fibroblasts, microvascular endothelial cells, monocytes and T-cells

International Journal of Molecular Sciences

Volumn 4, Issue 1, 2003, Pages 13-21

  Graham Evans, Barbara ^a   Tchounwou, Paul B ^a   Cohly, Hari H P ^b  

^a Jackson State University ^*  (United States)

^b UNIVERSITY OF MISSISSIPPI SCHOOL OF MEDICINE   (United States)

Author keywords

Arsenic; Cytotoxicity; Mitogenicity; Skin cells

Indexed keywords

ARSENIC TRIOXIDE; CARBON DIOXIDE; FISH OIL; FLUORESCEIN DIACETATE; PENICILLIN G; STREPTOMYCIN;

ARTICLE; CELL CULTURE; CELL PROLIFERATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DENDRITIC CELL; DRUG CYTOTOXICITY; ENDOTHELIUM CELL; FIBROBLAST; HUMAN; HUMAN CELL; INCUBATION TIME; INCUBATOR; KERATINOCYTE; LD 50; MELANOCYTE; MICROTITER PLATE ASSAY; MONOCYTE; SKIN CELL; T LYMPHOCYTE;

BOVINAE;

EID: 3042724849     PISSN: 14220067     EISSN: None     Source Type: Journal    
DOI: 10.3390/i4010013     Document Type: Article

References (18)

1. Agency for Toxic Substances and Disease Registry (ATSDR). Toxfacts for Arsenic (Update). Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service. 2000, online at http://www.atsdr.cdc.gov/tfacts2.html
2. U.S. Environmental Protection Agency. Integrated Risk Information System (IRIS) on Arsine. Environmental Criteria and Assessment Office, Office of Health and Environmental Assessment, Office of Research and Development, Cincinnati, OH. 1993.
3. World Health Organization (WHO). Arsenic in Drinking Water. Fact sheet No 210. Geneva, Switzerland, 2001
4. Gandolfi, A. J. Molecular Effects of Low Level Exposure to Arsenic, 2000. superfund-info@pharmacy.arizona.edu.
5. Quinby, G. Melanocytes and Melanogenesis. Case Western University, 2000. On line at: http://dermed.cwru.edu/residents/melanocyte.htm.
6. Waclavicek, M.; Berer, A.; Oehler, L.; Stockl, J.; Schloegl, E.; Majdic, O.; Knapp, W. Calcium ionophore: a single reagent for the differentiation of primary human acute myelogenous leukaemia cells towards dendritic cells. Br. J. Haematol. 2001, 114(2), 466-73.
7. Ali, A.; Lock, J. Physiological and toxicological changes in the skin resulting from the action and interaction of metal ions. Crit. Rev. Toxicol. 1995, 25, 397-462.
8. Chiang, M. C.; Yih, L. H.; Haung, R. N.; Peck, K.; Lee, T. C. Tumor formation of immortalized HaCaT cells in nude mice by long term exposure to sodium arsenite at non toxic doses. Toxicology 2001, 164, 95.
9. Chen, C-S. J.; Siegel, D. M. Arsenical keratosis. eMedicine J. 2001, 2(6).
10. Bae, D. S.; Gennings, C.; Carter, W. H. Jr.; Yang, R. S.; Campain, J. A. Toxicological interactions among arsenic, cadmium, chromium, and lead in human keratinocytes. Toxicol. Sci. 2001, 63, 132-142.
11. Germolec, D.R.; Yoshida, T.; Gaido, K.; Wilmer, J. L.; Simeonova, P. P.; Kayama, F.; Burleson, F.; Dong, W.; Lange, R. W.; Luster, M. I. Arsenic induces overexpression of growth factors in human keratinocytes. Toxicol. Appl. Pharmacol. 1996, 141, 308-318.
12. Vega, L.; Styblo, M.; Patterson, R.; Cullen, W.; Wang, C.; Germolec, D. Differential effects of trivalent and pentavalent arsenicals on cell proliferation and cytokine secretion in normal human epidermal keratinocytes. Toxicol. Appl. Pharmacol. 2001, 172(3), 225-232.
13. 3) on non-acute promyelocytic leukaemia myeloid cell lines: induction of apoptosis and inhibition of proliferation. Br. J. Haematol. 2002, 116, 555-563.
14. Trouba, K. J.; Glanzer, J. G.; Vorce, R. L. Wild-type and Ras-transformed fibroblasts display differential mitogenic responses to transient sodium arsenite exposure. Toxicol. Sci. 1999, 50, 72-81.
15. Chen, G. Q.; Zhu, J.; Shi, X. G.; Ni, J. H.; Zhong, H. J.; Si, G. Y.; Jin, X. L.; Tang, W.; Li, X. S.; Xong, S. M.; Shen, Z. X.; Sun, G. L.; Ma, J.; Zhang, P.; Zhang, T. D.; Gazin, C.; Naoe, T.; Chen, S. J.; Wang, Z. Y.; Chen, Z. In vitro studies on cellular and molecular mechanisms of arsenic trioxide (As2O3) in the treatment of acute promyelocytic leukemia: As2O3 induces NB4 cell apoptosis with downregulation of Bcl-2 expression and modulation of PML-RAR alpha/PML proteins. Blood 1996, 88, 1052-1061.
16. Jing, Y.; Dai, J.; Chalmers-Redman, R. M.; Tatton, W. G.; Waxman, S. Arsenic trioxide selectively induces acute promyelocytic leukemia cell apoptosis via a hydrogen peroxide-dependent pathway. Blood 1999, 94, 2102-2111.
17. Jia, P.; Chen, G.; Huang, X.; Cai, X.; Yang, J., Wang, L.; Zhou, Y.; Shen, Y.; Zhou, L.; Yu, Y.; Chen, S.; Zhang, X.; Wang, Z. Arsenic trioxide induces multiple myeloma cell apoptosis via disruption of mitochondrial transmembrane potentials and activation of caspase-3. Chin. Med. J. (Engl) 2001, 114, 19-24.
18. Vogt, B.; Rossman, T. Effects of arsenite on p53, p21 and cyclin D expression in normal human fibroblasts a possible mechanism for arsenite's comutagenicity. Mutat. Res. 2001, 478, 159-168.