Construction of a large single-chain phage antibody library

Chinese Journal of Microbiology and Immunology

Volumn 24, Issue 3, 2004, Pages 194-197

  Qiao, Yuan Yuan ^a   Wang, Yan ^a   Chen, Xiao Sui ^a   Wang, Yu Xiao ^a   Hua, Bing ^a  

^a NAVY GENERAL HOSPITAL   (China)

Author keywords

Loxp cre recombination system; Phage antibody library; Single chain antibody

Indexed keywords

ANTIGEN; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN;

ARTICLE; BACTERIUM; BLOOD; GENE AMPLIFICATION; GENE LIBRARY; GENE VECTOR; GENETIC RECOMBINATION; LYMPHOCYTE; MOLECULAR CLONING; NONHUMAN; PHAGE ANTIBODY LIBRARY; PHAGEMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; UMBILICAL CORD BLOOD;

EID: 3042723286     PISSN: 02545101     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (4)

1. Aujame L, Geoffroy F, Sodoyer R. High affinity human antibodies by phage display. Human Antibodies, 1997, 8: 155-168.
2. Griffiths AD, Williams SC, Hartley O, et al. Isolation of high affinity human antibodies directly from large synthetic repertoire. EMBO J, 1994, 13: 3245-3260.
3. Sblattero D, Bradbury A. Exploiting recombination in single bacteria to make large phage antibody libraries. Nat Biotechnol, 2000, 18: 74-80.
4. Sheets MD, Amersdorter P, Finnem R, et al. Efficient construction of a large nonimmune phage antibody library: the production of high-affinity human single-chain antibodies to protein antigens. Proc Natl Acad Sci USA, 1998, 95: 6157-6162.