Preparation of recombinant α-thrombin: High-level expression of recombinant human prethrombin-2 and its activation by recombinant ecarin

Journal of Biochemistry

Volumn 135, Issue 5, 2004, Pages 577-582

  Yonemura, Hiroshi ^a   Imamura, Takayuki ^a   Soejima, Kenji ^a   Nakahara, Yo ^a   Morikawa, Wataru ^a   Ushio, Yoshitaka ^a   Kamachi, Yasuharu ^a   Nakatake, Hiroshi ^a   Sugawara, Keishin ^a   Nakagaki, Tomohiro ^a   Nozaki, Chikateru ^a  

^a Chemo Sero Therapeutic Research Institute   (Japan)

Author keywords

thrombin; Ecarin; Prethrombin 2; Recombinant

Indexed keywords

BENZAMIDINE; BETA ACTIN; CHROMOGENIC SUBSTRATE; DIHYDROFOLATE REDUCTASE; ECARIN; FIBRINOPEPTIDE A; PEPTONE; PROTEIN C; PROTEIN PRECURSOR; RECOMBINANT ECARIN; RECOMBINANT HUMAN PRETHROMBIN 2; RECOMBINANT PROTEIN; RECOMBINANT THROMBIN; SEPHAROSE; THROMBIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CULTURE MEDIUM; ENZYME ACTIVATION; FED BATCH CULTURE; GENE AMPLIFICATION; GENE EXPRESSION SYSTEM; GENE MUTATION; HYDROLYSIS; MYELOMA CELL; NONHUMAN; PLASMID; PROMOTER REGION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SCALE UP;

ACTINS; ANIMALS; BIOTECHNOLOGY; BLOTTING, WESTERN; CATTLE; CELL LINE; CHICKENS; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CRICETINAE; DNA, COMPLEMENTARY; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ENZYME ACTIVATION; ENZYME PRECURSORS; GENETIC VECTORS; HUMANS; KINETICS; METHOTREXATE; MICE; MULTIPLE MYELOMA; MUTATION; PLASMIDS; PLATELET AGGREGATION; PROMOTER REGIONS (GENETICS); PROTHROMBIN; RECOMBINANT PROTEINS; SEPHAROSE; TETRAHYDROFOLATE DEHYDROGENASE; THROMBIN; TIME FACTORS; TRANSFECTION;

ANIMALIA; BOVINAE; GALLUS GALLUS;

EID: 3042709224     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvh070     Document Type: Article

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